中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8476-8480.doi: 10.3969/j.issn.2095-4344.2012.45.021

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脂肪源性干细胞移植急性心肌梗死后心脏缝隙连接蛋白43的表达

揭领军1，王挹青1，2，马群超2，陈晓敏2，罗 宏2，张成炜1   

  1. 1福建医科大学协和临床医学院，福建省福州市 350001
    2厦门市心脏中心心内科，福建省厦门市 361004
  • 收稿日期:2012-04-05 修回日期:2012-04-24 出版日期:2012-11-04 发布日期:2012-11-04
  • 通讯作者: 王挹青，博士，主任医师，教授，福建医科大学协和临床医学院，福建省福州市 350001；厦门市心脏中心心内科，福建省厦门市 361004 wang_gina@163.com
  • 作者简介:揭领军★，男，1984年生，江西省南昌市人，汉族，福建医科大学在读硕士，主要从事心肌梗塞干细胞治疗研究。 jielingjun11@163.com

Connexin-43 expression in cardiac tissue after adipose-derived stem cells transplantation for treatment of acute myocardial infarction

Jie Ling-jun1, Wang Yi-qing1,2, Ma Qun-cao2, Chen Xiao-min2, Luo Hong2, Zhang Cheng-Wei1   

  1. 1Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China
    2Department of Cardiology, Xiamen Heart Centre, Xiamen 361004, Fujian Province, China
  • Received:2012-04-05 Revised:2012-04-24 Online:2012-11-04 Published:2012-11-04
  • Contact: Wang Yi-qing, M.D., Chief physician, Professor, Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China; Department of Cardiology, Xiamen Heart Centre, Xiamen 361004, Fujian Province, China wang_gina@163.com
  • About author:Jie Ling-jun★, Studying for master’s degree, Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China jielingjun11@163.com

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691

被引次数

1

摘要：

背景：脂肪源性干细胞作为一种新型的种子细胞，移植后对大鼠心肌梗死治疗作用的研究报道较少。
目的：观察脂肪源性干细胞移植后对急性心肌梗死大鼠心功能和缝隙连接蛋白43表达的影响。
方法：分离培养SD大鼠脂肪源性干细胞。将SD大鼠分为3组，心肌梗死组、脂肪源性干细胞移植组均建立急性心肌梗死模型，假手术组开胸不结扎。脂肪源性干细胞移植组于心肌梗死后30 min心肌内分4点注射脂肪源性干细胞，每点25 μL；其他2组分别注射等量的PBS。移植2周后行相应指标观察。
结果与结论：心脏彩超结果显示，与心肌梗死组相比，脂肪源性干细胞移植组的左室射血分数、短轴缩短率均上升(P < 0.05)。马森三色染色结果显示，脂肪源性干细胞移植组纤维渗出率明显低于心肌梗死组(P < 0.05)。免疫荧光结果显示，与心肌梗死组比较，脂肪源性干细胞组缝隙连接蛋白43表达和血管密度均显著上升(P < 0.05-0.01)。实时聚合酶链反应结果表明，脂肪源性干细胞移植组缝隙连接蛋白43 mRNA的表达高于心肌梗死组(P < 0.01)。提示脂肪源性干细胞移植不仅可以显著改善心肌梗死后心功能，还可以减少纤维渗出，上调缝隙连接蛋白43的表达。

关键词: 心肌梗死, 脂肪源性干细胞, 细胞移植, 心功能修复, 微血管密度, 缝隙连接蛋白, 干细胞

Abstract:

BACKGROUND: The therapeutic effect of adipose-derived stem cells (ADSC) as a novel kind of seed cells on myocardial infarction in rats has been rarely reported.
OBJECTIVE: To investigate the effects of ADSCs transplantation on cardiac function and connexin-43 expression in rats with acute myocardial infarction.
METHODS: Rat ADSCs were isolated and cultured in vitro. Twenty-four Sprague-Dawley rats were divided into sham-operated, myocardial infarction and ADSCs groups. Rats in the former two groups were prepared into acute myocardial infarction models by ligation of the left anterior descending branch. In the sham-operated group, only chest was cut open without ligation. In the ADSCs group, at 30 minutes after myocardial infarction, ADSCs were administered via four points, 25 μL per point. Rats in the sham-operated and myocardial infarction groups received identical amounts of phosphate buffered saline. At 2 weeks after ADSCs transplantation, index examination was performed.
RESULTS AND CONCLUSION: Echocardiogram showed that left ventricular ejection fraction, and left ventricular fractional shortening were significantly greater in the myocardial infarction group than in the ADSCs group (P < 0.05). Masson’s trichrome staining demonstrated that fibrinous exudate level was significantly lower in the ADSCs group than in the myocardial infarction group (P < 0.05). Immunofluorescent results showed that connexin-43 expression and vascular density in the ADSCs group were significantly increased than in the myocardial infarction group (P < 0.05-0.01). Real-time PCR showed that connexin-43 mRNA expression in the ADSCs group was significantly greater than in the myocardial infarction group (P < 0.01). ADSCs transplantation can greatly improve the cardiac function, reduce fibrinous exudates and up-regulate connexin-43 expression after myocardial infarction.

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