中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8481-8485.doi: 10.3969/j.issn.2095-4344.2012.45.022

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

5 μg/L碱性成纤维细胞生长因子促脂肪来源干细胞的增殖

陈 阳，高建华，察鹏飞，鲁 峰   

  1. 福州市皮肤病防治院，福建省福州市 350025
  • 收稿日期:2012-02-07 修回日期:2012-02-29 出版日期:2012-11-04 发布日期:2013-03-16
  • 作者简介:陈阳☆，男，1966年生，福建省莆田市人，汉族，南方医科大学毕业，博士，主任医师，主要从事汉族瘢痕疙瘩家系易感基因的定位研究、成人脂肪来源干细胞构建脂肪组织工程研究和国内医疗纠纷解决机制的法律问题研究。 cyfuzhou2008@126.com

5 μg/L basic fibroblast growth factors promote the proliferation of adipose-derived stem cells

Chen Yang, Gao Jian-hua, Cha Peng-fei, Lu Feng   

  1. Fuzhou Hospital for Dermatosis, Fuzhou 350025, Fujian Province, China
  • Received:2012-02-07 Revised:2012-02-29 Online:2012-11-04 Published:2013-03-16
  • About author:Chen Yang☆, M.D., Chief physician, Fuzhou Hospital for Dermatosis, Fuzhou 350025, Fujian Province, china cyfuzhou2008@126.com

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摘要：

背景：碱性成纤维细胞生长因子作为一种具有强烈促进新生血管生成和脂肪细胞增殖分化作用的细胞因子，在临床上广泛应用于自体脂肪移植术。
目的：观察不同质量浓度的碱性成纤维细胞生长因子对脂肪来源干细胞促增殖的影响，分析促进脂肪来源干细胞增殖的适合浓度。
方法：从抽脂术得到的脂肪组织当中分离培养脂肪来源干细胞，观察细胞形态及生长情况。分别配制含有0，5，10，20，40，80，160 μg/L 碱性成纤维细胞生长因子的培养液培养脂肪干细胞。连续7 d应用MTT法检测脂肪来源干细胞的增殖。
结果与结论：碱性成纤维细胞生长因子有明显促进脂肪来源干细胞增殖的作用，第3 天开始出现明显的促增殖作用，第5，6 天达到高峰。实验组各质量浓度之间对脂肪来源干细胞的促增殖作用无明显差异，周围环境中碱性成纤维细胞生长因子的质量浓度达到5 μg/L即可对脂肪来源干细胞起到明显的促进作用，增加剂量促增殖作用无明显改变。

关键词: 碱性成纤维细胞生长因子, 脂肪来源干细胞, MTT, 细胞增殖, 细胞培养

Abstract:

BACKGROUND: Basic fibroblast growth factors have been widely used for clinical autologous fat grafting because they exhibit great potential to promote angiogenesis and adipocyte proliferation and differentiation.
OBJECTIVE: To investigate the effects of different concentrations of basic fibroblast growth factors on proliferation of adipose-derived stem cells and analyze the proper concentration of basic fibroblast growth factors for promoting proliferation of adipose-derived stem cells.
METHODS: Adipose-derived stem cells were isolated from adipose tissue by liposuction. Cell morphology and growth characteristics were observed under the microscope. Culture medium containing 0, 5, 10, 20, 40, 80, 60 μg/L basic fibroblast growth factors was prepared. Adipose-derived stem cells were cultured in a 96-well plate at 103 cells/well using culture medium containing basic fibroblast growth factors. The proliferation of adipose-derived stem cells was determined by MTT for successive 7 days.
RESULTS AND CONCLUSION: Basic fibroblast growth factors could promote the proliferation of adipose-derived stem cells significantly. The proliferation-promoting effect was significant since the 3rd day and peaked on the 5th and 6th days. There was no obvious difference in proliferation-promoting effects between different basic fibroblast growth factor concentrations. 5 μg/L was the most appropriate concentration for basic fibroblast growth factors in promoting the proliferation of adipose-derived stem cells.

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