中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (36): 6767-6773.doi: 10.3969/j.issn.2095-4344.2012.36.021

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

羊膜间充质干细胞向运动神经元前体细胞的分化

胡 炜1，2，杨 枫1，唐尤佳1，杨 波2，关方霞3   

  1. 1九江市第一人民医院神经外科，江西省九江市332000；
    2郑州大学第一附属医院神经外科，河南省郑州市 450052；
    3郑州大学生物工程系，河南省郑州市 450001
  • 收稿日期:2011-10-02 修回日期:2011-11-16 出版日期:2012-09-02 发布日期:2012-09-02
  • 通讯作者: 杨波，博士，主任医师，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市450052 yangbo96@126.com
  • 作者简介:胡炜☆，男，1971年生，河南省开封县人，汉族，2009年郑州大学毕业，博士，副主任医师，主要从事干细胞培养、移植和神经系统损伤修复方面的研究。 huwei18120@sina.com

Amniotic mesenchymal stem cells differentiate into motor neuron precursor cell

Hu Wei1, 2, Yang Feng1, Tang You-jia1, Yang Bo2, Guan Fang-xia3   

  1. 1Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang 332000, Jiangxi Province, China;
    2Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China;
    3Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China
  • Received:2011-10-02 Revised:2011-11-16 Online:2012-09-02 Published:2012-09-02
  • Contact: 杨波，博士，主任医师，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市450052 yangbo96@126.com
  • About author:Hu Wei☆, Doctor, Associate chief physician, Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang 332000, Jiangxi Province, China huwei18120@sina.com

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375

被引次数

2

摘要：

背景：成体干细胞在细胞外基质、细胞因子分步诱导下的神经分化，尚少见报道。
目的：探索天然膜样细胞外基质加多种细胞因子诱导作用下，人羊膜间充质干细胞体外的神经分化情况。
方法：健康人羊膜分离培养羊膜间充质干细胞，酶、化学方法制作膜样细胞外基质并检测其生物相容性。将细胞分为2组，实验组细胞接种在包被膜样基质玻片的24孔板中，更换不同培养基分步诱导分化；对照组去除膜样基质，其余方法同实验组。
结果与结论：实验组细胞神经元特异性烯醇化酶、兔抗人突触蛋白表达升高，神经胶质纤维酸性蛋白表达降低，兔抗人突触蛋白表达明显高于对照组，对照组神经元特异性烯醇化酶表达升高，其余无明显变化。第1代羊膜间充质干细胞不同程度表达胚胎干细胞和神经祖细胞标志，诱导后实验组各转录因子均有变化。说明实验所用方法提取的细胞外基质具有良好的生物相容性，可以促进羊膜间充质干细胞神经分化过程中的突触成熟，分步诱导的方法可以使羊膜间充质干细胞向运动神经元前体细胞分化。

关键词: 动物模型, 脊髓损伤, 羊膜间充质干细胞, 神经分化, 干细胞膜

Abstract:

BACKGROUND: The reports about neural differentiation of adult stem cells induced by extracellular matrix and cytokines are rarely reported.
OBJECTIVE: To explore the neural differentiation of human amniotic mesenchymal stem cells in vitro induced by natural membrane-like extracelluler martrix.
METHODS: Amniotic mesenchymal stem cells were isolated from healthy human amnion, and membrane-like extracellular matrix was made by enzyme digestion and chemical method and biocompatibility was detected. The cells were divided into two groups. In the experimental group, the cells were seeded in 24-well plates collated with capsule-like matrix slides and culture medium was changed. In the control group, membrane-like matrix was removed and the other procedures were the same as the experimental group.
RESULTS AND CONCLUSION: After step-by-step induction in the experimental group, the expressions of neuron-specific enolase and rabbit anti-human synaptic proteins were increased and the expression of neuroglial fibrillary acidic protein was decreased, the expression of rabbit anti-human synaptic protein in the experimental group was significantly higher than that in the control group. In the control group, the expression of neuron-specific enolase was increased significantly and the expression of rabbit anti-human synaptic protein and neuroglial fibrillary acidic protein were not changed. Passage 1 amniotic mesenchymal stem cells could express the marker of embryonic stem cells and neural progenitor cells, the transcription factors in the experimental group were changed after induction. It indicates that the extracellular matrix extracted by step-by-step method had a good biocompatibility, which could promote the mature of synapses during the differentiation of amniotic mesenchymal stem cells. Induction by step-by-step method could enrich neural progenitor cells in amniotic mesenchymal stem cells and promote them to differentiate into motor neuron-like precursor cells.

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