中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5196-5199.doi: 10.3969/j.issn.2095-4344.2012.28.014

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

Y透明剂替代二甲苯在病理制片中的应用

孙 帅1，赖 洵2   

  1. 云南省第一人民医院，1心内科， 2血液内科，云南省昆明市 650032
  • 收稿日期:2012-01-29 修回日期:2012-06-08 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 赖洵，硕士生导师，主任医师，云南省第一人民医院血液内科，云南省昆明市 650032
  • 作者简介:孙帅☆，1975年生，山东省青岛市人，2010年昆明医科大学毕业，博士，副主任医师，主要从事心血管病基础研究。 alice1191@sina.com

Application of Y clearing agent, a substitute of xlylene, in pathological section preparation

Sun Shuai1, Lai Xun2   

  1. 1Department of Cardiology,
    2Department of Hematology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Received:2012-01-29 Revised:2012-06-08 Online:2012-07-08 Published:2012-07-08
  • Contact: Lai Xun, Master’s supervisor, Chief physician, Department of Hematology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • About author:Sun Shuai☆, Doctor, Associate chief physician, Department of Cardiology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China alice1191@sina.com

PDF

499

被引次数

3

摘要：

背景：寻找替代二甲苯的透明剂，是避免二甲苯中毒的惟一方法。既往使用松节油、TO透明剂、 Hisboelear和Solvent CNP30等用做透明剂，终因发挥性不好，溶蜡能力差，刺激性气味强烈，资源有限，价格昂贵，酸价太高或抗氧化能力等指标不理想，未能在制片上完全取代二甲苯。
目的：探讨Y透明剂用作病理组织石蜡切片透明剂的可行性。
方法：取大鼠肠、睾丸、肺、肾脏不同类型组织及临床肝、网膜、脾、肺、肾、皮肤、子宫刮宫物、胎盘、脾或血栓物多种组织做标本，经过组织固定、脱水、透明、浸蜡、包埋、切片、脱蜡、贴片及烤片等步骤，分别用Y透明剂和二甲苯制作组织切片。
结果与结论：用Y透明剂制作的组织切片，透明效果优于二甲苯。使用常规苏木精-伊红染色法及特殊染色法的染色效果与常规制片无明显差异。结果可见无毒新型Y透明剂可以完全替代二甲苯用于病理制片。

关键词: 二甲苯, Y透明剂, 病理制片, 组织切片, 透明效果, 透明剂, 生物材料

Abstract:

BACKGROUND: Finding clearing agents replacing xylene is the only way to avoid xylene poisoning. Turpentine, TO clearing, Hisboelear and Solvent CNP30 are used as clearing agents, but they can not completely replace xylene for their poor volatility and melting wax capacity, strong pungent smell, limited resource, expensive price, high acid value and poor antioxidant capacity.
OBJECTIVE: To explore the feasibility of Y clearing as the clearing agent for paraffin section of pathological tissues.
METHODS: The tissues of the intestine, testicle, lung and kidney from rats as well as the liver, omentum, spleen, lung, kidney, skin, tissues from uterine curettage, placenta, or thrombus from human being were sampled. These samples were treated with many procedures, including fixation, dehydration, clearing, infiltration, embedding, section, dewaxing and patching. And then, tissue sections were prepared by Y clearing agent and xylene, respectively.
RESULTS AND CONLUSION: The tissue sections prepared by Y clearing agent were superior to those prepared by xylene. There was no significant difference in staining effects between hematoxylin-eosin staining and special staining. These findings suggest that the new-type non-toxic Y clearing agent can completely replace xylene for pathological section.

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