中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5200-5205.doi: 10.3969/j.issn.2095-4344.2012.28.015

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

相关系数分析法在滚动细胞数据筛选中的应用

安丽丽，李趣欢，方 颖   

  1. 华南理工大学，生物科学与工程学院，广东省广州市 510006
  • 收稿日期:2012-02-29 修回日期:2012-05-05 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 方颖，副教授，硕士生导师，华南理工大学生物科学与工程学院，广东省广州市 510006 yfang@scut.edu.cn
  • 作者简介:安丽丽★，女，1986年生，山东省泰安市人，汉族，华南理工大学在读硕士，主要从事生物力学方面的研究。 anlili12@163.com 并列第一作者：李趣欢☆，女，1979年生，广东省佛山市人，汉族，2009年中山大学毕业，博士，讲师，主要从事细胞与分子生物力学领域的研究。 liqu@scut.edu.cn

Application of correlation coefficient analysis in rolling cell data screening

An Li-li, Li Qu-huan, Fang Ying   

  1. Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China
  • Received:2012-02-29 Revised:2012-05-05 Online:2012-07-08 Published:2012-07-08
  • Contact: Fang Ying, Associate professor, Master’s supervisor, Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China yfang@scut.edu.cn
  • About author:An Li-li★, Studying for master’s degree, Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China anlili12@163.com Li Qu-huan, Doctor, Lecturer, Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China liqu@sart. edu. cn An Li-li and Li Qu-huan contributed equally to this study

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摘要：

背景：常用的传统人工筛选流动腔实验数据的方法繁琐费时并有一定的主观性。
目的：建立一种基于相关系数的高效数据筛选方法，应用于流动腔实验的细胞滚动黏附数据的筛选中。
方法：以E-选择素/配体介导的人早幼粒白血病细胞的滚动为模型，运用相关系数分析法筛选稳定滚动细胞，分析平行平板流动腔实验中稳定滚动的细胞的瞬时速度和位移数据，统计细胞滚动的平均速度，并与人工筛选的结果进行比较。
结果与结论：相关系数法筛选目标与人工筛选目标得到的细胞滚动速度结果相吻合。在剪切力0.01~0.07 Pa范围内，平均速度对力的依赖关系表现为两阶段曲线，即随剪切力的增大，平均速度呈现先减小后增大趋势，符合实验观察事实，表明该方法具有可行性。提示相关系数法可以作为筛选流动腔实验数据的重要手段，并代替传统人工筛选法。

关键词: 相关系数, 平行平板流动腔, 数据筛选, 瞬时速度, 位移, 人早幼粒白血病细胞

Abstract:

BACKGROUND: The traditional method of screening the flow chamber experimental data is to pick up each objective cell manually, which is cumbersome, time-consuming and subjective.
OBJECTIVE: To develop an efficient data screening method based on correlation coefficient analysis, which is applied to screen the cell rolling adhesion data from flow chamber experiments.
METHODS: E-selectin/ligand-mediated HL-60 cells rolling was chosen to be a model and correlation coefficient analysis was used to screen the stable rolling cells from parallel-plate flow chamber experiments. Then the instantaneous velocities and displacements of stable rolling cells were measured by software to calculate the average rolling velocities of these cells, which are compared with the data screened by the traditional manual method.
RESULTS AND CONCLUSION: The rolling velocities of stable rolling cells screened by correlation coefficient method were consistent with those screened by the traditional manual method. When shear stress was in the range of 0.01-0.07 Pa, the relationship between the average velocities of stable rolling cells and the shear stress displayed a two-stage curve, in which the average velocities first decreased and then increased with the monotonically increasing of shear stress. Therefore, the comparisons showed the biophysical phenomenon observed in experiments and indicated that the correlation coefficient method was feasible to screen the stable rolling cells of parallel plate flow chamber experiments. Correlation coefficient screen method can be applied to parallel flow chamber experimental data and take the place of the traditional manual screen method.

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