中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (17): 2770-2775.doi: 10.3969/j.issn.2095-4344.1695

• 干细胞综述 stem cell review • 上一篇    下一篇

脑脊液促进间充质干细胞神经元样分化的特点

任 超1,冀永强2,管丽娜3   

  1. 青岛大学附属烟台毓璜顶医院,1神经内科,2肾内科,3神经科重症监护室,山东省烟台市 264000
  • 修回日期:2019-01-16 出版日期:2019-06-18 发布日期:2019-06-18
  • 通讯作者: 管丽娜,硕士,主治医师,青岛大学附属烟台毓璜顶医院神经科重症监护室,山东省烟台市 264000
  • 作者简介:任超,男,1986年生,江苏省徐州市人,汉族,2017年徐州医学院硕士毕业,主治医师,主要从事神经变性疾病研究。
  • 基金资助:

    国家自然科学基金青年科学基金项目(81501185),项目负责人:任超;山东省重点研发计划(公益类专项)项目(2017GSF218043),项目负责人:任超;烟台市重点研发计划(2016WS017),项目负责人:管丽娜

Cerebrospinal fluid promotes neuron-like differentiation of mesenchymal stem cells

Ren Chao1, Ji Yongqiang2, Guan Lina3   

  1. 1Department of Neurology, 2Department of Nephrology, 3Department of Neurology Intensive Care Unit, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yanta 264000, Shandong Province, China
  • Revised:2019-01-16 Online:2019-06-18 Published:2019-06-18
  • Contact: Guan Lina, Master, Attending physician, Department of Neurology Intensive Care Unit, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yanta 264000, Shandong Province, China
  • About author:Ren Chao, Master, Attending physician, Department of Neurology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yanta 264000, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China (Youth Science Fund Project), No. 81501185 (to RC); Shandong Key Research and Development Program (Public Welfare Special Project), No. 2017GSF218043 (to RC); Yantai City Key Research and Development Plan, No. 2016WS017 (to GLN)

摘要:

文章快速阅读:

文题释义:
骨髓间充质干细胞的分化诱导:
骨髓间充质干细胞具有多向分化和增殖能力,且免疫原性低,易于获得、分离和培养,研究者认为神经细胞生长过程中可分泌一些诱导骨髓间充质干细胞分化的物质,其提供的微环境对骨髓间充质干细胞分化为神经元具有促进作用。
脑脊液的干细胞诱导功能:脑脊液既可营养神经细胞,又可成为一种有效、适宜的诱导剂为神经干细胞的获得增加新的途径。脑脊液中的因子通过细胞表面相应的受体可促进间充质干细胞向神经细胞增殖和分化,提示间充质干细胞的分化具有组织特异性。

 

摘要
背景:
目前研究表明对内源神经干细胞进行调控,或进行外源神经干细胞移植,是治疗痴呆等神经系统疾病最新和最有希望的方法。其中外源其他组织来源干细胞向神经干细胞定向分化的成功,为神经干细胞的来源增加了新途径。近年研究表明脑脊液既可营养神经细胞,又可成为一种有效、适宜的诱导剂,为神经干细胞的获得增加新的途径。
目的:文章将对国内外脑脊液对干细胞影响的相关研究进行综述,以期为将来神经干细胞临床应用提供帮助。
方法:以“Cerebrospinal fluid,Stem cells,Induced differentiation,Neural stem cells,Stem cell transplantation therapy”为英文关键词,通过计算机检索PubMed数据库与Embase数据库;以“脑脊液、干细胞、诱导分化、增殖、分化、神经干细胞、间充质干细胞、胚胎干细胞、银杏叶提取物、干细胞移植治疗、神经变性疾病”为中文关键词,计算机检索CNKI数据库,万方数据库,维普数据库等科技期刊数据库;检索时间为1998-12-10至2018-12-10。对脑脊液对间充质干细胞、胚胎干细胞及神经干细胞等的影响进行总结分析,为将来神经干细胞临床应用提供帮助。
结果与结论:①“脑脊液-干细胞”相互作用是治疗神经系统疾病的希望;②脑脊液也能促进间充质干细胞分化成为神经元样细胞;③脑脊液能诱导人胚胎源干细胞分化为神经样细胞,但所得不同类型细胞的比例不同;④脑脊液能诱导内源性神经干细胞的增殖分化。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:
0000-0002-5418-3354(任超)

关键词: 脑脊液, 干细胞, 诱导分化, 增殖, 分化, 神经干细胞, 间充质干细胞, 胚胎干细胞, 银杏叶提取物, 干细胞移植治疗, 神经变性疾病

Abstract:

BACKGROUND: Current research indicates that regulation of endogenous neural stem cells or transplantation of exogenous neural stem cells is the latest and most promising method for the treatment of neurological diseases such as dementia. The success of the differentiation of stem cells from other sources into neural stem cells has provided a new way for the source of neural stem cells. Recent studies have shown that cerebrospinal fluid cannot only nourish nerve cells, but also become an effective and suitable inducer to add new ways for the acquisition of neural stem cells.
OBJECTIVE: To review the effects of cerebrospinal fluid on stem cells at home and abroad, in order to provide assistance for the clinical application of neural stem cells in the future.
METHODS: “Cerebrospinal fluid, stem cells, induced differentiation, neural stem cells, stem cell transplantation therapy” were used as English keywords to search the PubMed database and the Embase database by computer. “Cerebrospinal fluid, stem cells, induced differentiation, proliferation, differentiation, neural stem cells, mesenchymal stem cells, embryonic stem cells, ginkgo biloba extract, stem cell transplantation therapy, neurodegenerative diseases” were used as Chinese keywords to search CNKI database, WanFang database, VIP database and other scientific journal databases. The search time was from December 10th, 1998 to December 10th, 2018. The effects of cerebrospinal fluid on mesenchymal stem cells, embryonic stem cells, and neural stem cells were summarized and analyzed, providing assistance for the clinical use of neural stem cells in the future.
RESULTS AND CONCLUSION: (1) Interaction of cerebrospinal fluid and stem cells is the hope of treating nervous system diseases. (2) Cerebrospinal fluid can promote the differentiation of mesenchymal stem cells into neuron-like cells. (3) Cerebrospinal fluid can induce human embryonic stem cells to differentiate into neuron-like cells, but the proportion of different types of cells obtained is different. (4) Cerebrospinal fluid can induce the proliferation and differentiation of endogenous neural stem cells.

Key words: cerebrospinal fluid, stem cells, induced differentiation, proliferation, differentiation, neural stem cells, mesenchymal stem cells, embryonic stem cells, ginkgo biloba extract, stem cell transplantation therapy, neurodegenerative diseases

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