中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (14): 2162-2168.doi: 10.3969/j.issn.2095-4344.1673

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

3D打印β-磷酸三钙支架复合淫羊藿苷微粒修复兔股骨头坏死

彭晨健，杜  斌，孙光权，刘  锌，薛  鹏，曹良权   

  1. 南京中医药大学附属医院，江苏省南京市 210029
  • 收稿日期:2018-12-26 出版日期:2019-05-18 发布日期:2019-05-18
  • 通讯作者: 杜斌，教授，主任医师，博士生导师，南京中医药大学附属医院骨伤科，江苏省南京市 210029
  • 作者简介:彭晨健，男，1993年生，湖北省襄阳市人，汉族，硕士，主要从事骨科关节与运动医学方向研究。
  • 基金资助:

    江苏省卫计委中医药管理局项目(YB2015025)--控释型淫羊藿苷-壳聚糖/羟基磷灰石复合材料对兔股骨头坏死模型骨修复影响的实验研究，项目负责人：杜斌

Three-dimensional printing beta-tricalcium phosphate scaffold loaded with icariin particles for repairing osteonecrosis of the femoral head in rabbits

Peng Chenjian, Du Bin, Sun Guangquan, Liu Xin, Xue Peng, Cao Liangquan   

  1. Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Received:2018-12-26 Online:2019-05-18 Published:2019-05-18
  • Contact: Du Bin, Professor, Chief physician, Doctoral supervisor, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • About author:Peng Chenjian, Master, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Supported by:

    the Administration of Chinese Medicine of Health and Family Planning Commission of Jiangsu Province, No. YB2015025 (to DB)

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摘要：

文章快速阅读：

 

文题释义：
β-磷酸三钙：具有良好的生物相容性，植入人体后能在体内降解，并在降解过程中使周围内环境富含Ca、P，其弹性模量与松质骨相近，力学强度良好，目前已被证明其是一种良好的骨修复材料。
淫羊藿苷：淫羊藿为中医传统补益扶正药，具有补肾、强筋骨、壮腰膝、益心力的作用。淫羊藿苷是在淫羊藿的茎叶中提取的，具有良好的促进骨修复及血管形成的能力，促进成骨的同时可抑制破骨细胞分化，减少骨吸收。
 
 
背景：前期研究制备了3D打印β-磷酸三钙复合淫羊藿苷支架。
目的：观察3D打印β-磷酸三钙复合淫羊藿苷支架在兔股骨头坏死模型中的修复作用。
方法：取新西兰大白兔(南京青龙山实验动物中心提供)，制作激素型股骨头坏死模型，将造模成功的27只兔进行髓芯减压及清理死骨，随机分3组，分别植入自体骨(自体骨组)、β-磷酸三钙支架(磷酸三钙组)及3D打印β-磷酸三钙复合淫羊藿苷支架(复合支架组)。植入后第4，8，12周，进行股骨头micro-CT扫描及病理组织观察。

结果与结论：①micro-CT显示，植入第4周，3组植入物周围及内部均有骨小梁生成；自体骨组植入后第8周植骨区有大量骨小梁，植入后第12周骨小梁结构致密；磷酸三钙组、复合支架组支架与骨界面整合良好，复合支架组植入第4周时支架附近有一定量的骨小梁生成，至第8周时支架上有骨小梁长入，磷酸三钙组植入第4周时仅可见微量薄弱骨小梁，第8周时才可见支架周围广泛骨小梁生成，第12周时骨小梁仍较其他两组稀疏；②植入第12周苏木精-伊红染色显示，自体骨、复合支架组成骨细胞分化成熟，软骨基质较少，新生骨组织与植入物整合良好，磷酸三钙组仍存在较多的软骨基质，新生骨与植入物尚未完成整合；③植入第12周Masson染色显示，复合支架组成骨细胞增殖低于自体骨组(P < 0.05)，但高于磷酸三钙组(P < 0.05)；④植入第12周TRAP染色显示，复合支架组破骨细胞数少于磷酸三钙组(P < 0.05)，与自体骨组比较无差异(P > 0.05)；⑤植入第12周免疫组织化学染色显示，复合支架组血管内皮生长因子阳性率高于磷酸三钙组(P < 0.05)，但低于自体骨组(P < 0.05)；⑥结果表明，3D打印β-磷酸三钙复合淫羊藿苷支架植入股骨头坏死模型兔体内，可促进成骨细胞增殖分化、抑制破骨细胞活性，同时促进新生血管生成，具有促进兔股骨头坏死修复的作用。

ORCID: 0000-0002-1314-3997(彭晨健)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 股骨头坏死, 骨替代材料, 骨修复, 3D打印骨组织工程支架, 淫羊藿苷, 淫羊藿苷-β-磷酸三钙支架

Abstract:

BACKGROUND: Preliminary study has prepared three-dimensional printing β-tricalcium phosphate scaffold loaded with icariin.

OBJECTIVE: To investigate the role of three-dimensional printing β-tricalcium phosphate scaffold loaded with icariin in the repair of rabbit models of osteonecrosis of the femoral head.
METHODS: New Zealand white rabbits (provided by Qinglongshan Laboratory Animal Center of Nanjing) were selected to establish the steroid-induced osteonecrosis of the femoral head. The 27 model rabbits underwent core decompression and debridement, were randomly divided into three groups, and then implanted with autologous bone, β-tricalcium phosphate scaffold, three-dimensional printing β-tricalcium phosphate scaffold loaded with icariin (composite scaffold group), respectively. The micro-CT scanning and pathological observation were performed at 4, 8, and 12 weeks after implantation.
RESULTS AND CONCLUSION: (1) Micro-CT showed that at 4 weeks after implantation, trabecular bone was observed around and in implants in each group. In the autologous bone group, there were a large number of trabecular bones in the grafting area at 8 weeks, and the trabecular bone structure was dense at 12 weeks after implantation. In the tricalcium phosphate and composite scaffold groups, the scaffolds were well integrated with the bone interface. At 4 weeks after implantation, there was a certain amount of trabecular bone surrounding the scaffold, and trabecular grew into the scaffold until 8 weeks in the composite scaffold group. At 4 weeks after implantation, few thin trabecular bone was visible, and extensive trabecular bone formation was observed around the scaffold at 8 weeks in the tricalcium phosphate group. (2) Hematoxylin-eosin staining results showed that there were many mature osteoblasts, and few cartilage matrix, newly born bones integrated well to the implants at 12 weeks in the autologous bone and tricalcium phosphate groups. In the composite scaffold group, there were many cartilage matrixes, and newly born bones integrated poorly to the implants. (3) Masson staining showed that at 12 weeks after implantation, the osteogenic capacity in the composite scaffold group was lower than that in the autologous bone group (P < 0.05), but higher than that in the tricalcium phosphate group (P < 0.05). (4) TRAP staining results at 12 weeks after implantation revealed that the amount of osteoclast in composite scaffold group was less than that in the tricalcium phosphate group (P < 0.05), and was not significantly different from the autologous bone group (P > 0.05). (5) Immunohistochemical staining at 12 weeks after implantation revealed that the positive rate of vascular endothelial growth factor in the composite scaffold group was higher than that in the tricalcium phosphate group (P < 0.05), and lower than that in the autologous bone group (P < 0.05). (6) In summary, three-dimensional printing β-tricalcium phosphate scaffold loaded with icariin implanted into the rabbit model of osteonecrosis of the femoral head can promote the proliferation and differentiation of osteoblasts, inhibit the viability of osteoclasts, promote the angiogenesis, and contribute to the repair of osteonecrosis of the femoral head in rabbits.

Key words: Femur Head Necrosis;, Drugs, Chinese Herbal;, Calcium Phosphates, Tissue Engineering

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