中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (3): 372-377.doi: 10.3969/j.issn.2095-4344.0588

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

大鼠高加速度离心加载装置：高加速度环境下动物的力学生物学响应

高子剑1，于  露2，3，高丽兰1，张春秋1，张西正2   

  1.  (1天津市先进机电系统设计与智能控制重点实验室，机电工程国家级实验教学示范中心，天津理工大学机械工程学院，天津市  300384；2军事医学科学院卫生装备研究所，天津市  300000；3太原理工大学应用力学与生物医学工程研究所，山西省太原市  030000)
  • 收稿日期:2018-07-27 出版日期:2019-01-28 发布日期:2019-01-28
  • 通讯作者: 张春秋，博士，教授，天津市先进机电系统设计与智能控制重点实验室，天津理工大学机械工程学院，天津市 300384
  • 作者简介:高子剑，男，1992年生，山东省淄博市人，汉族，天津理工大学在读硕士，主要从事生物力学研究。
  • 基金资助:

    国家自然科学重点基金(11432016)，项目负责人：张西正；国家自然科学基金面上项目(81741141)，
    项目负责人：张春秋

A centrifuge device for high acceleration loading in rats: mechanobiological responses of animals in a high-acceleration environment

Gao Zijian1, Yu Lu2,3, Gao Lilan1, Zhang Chunqiu1, Zhang Xizheng2   

  1.  (1Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China; 2Institute of Medical Equipment of Academy of Military Medical Sciences, Tianjin 300000, China; 3Institute for Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030000, Shanxi Province, China)
  • Received:2018-07-27 Online:2019-01-28 Published:2019-01-28
  • Contact: Zhang Chunqiu, PhD, Professor, Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • About author:Gao Zijian, Master candidate, Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, National Experimental Teaching Demonstration Center of Mechatronics Engineering, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • Supported by:

    the National Natural Science Foundation of China (Key Program), No. 11432016 (to ZXZ); the National Natural Science Foundation of China (General Program), No. 81741141 (to ZCQ) 

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摘要：

文章快速阅读：

文题释义：
相对离心力(高加速度值)：相对离心力指在离心力场的作用下，物体所受离心力相当于地球重力的倍数，单位是重力加速度(9.8 m/s2)，由于不同离心机转子的半径或者离心管至旋转轴中心的距离不同，所以离心力也不同，因此文献中常用“相对离心力”或“数字×g”表示离心力。
高G条件引起意识丧失(G-LOC)：航空航天生理学中，持续的高G环境会使人的大脑缺血导致缺氧引起意识丧失。正高G环境，会使人的血液从头部流向脚部，人处在更高强度或更长时间的正高G环境中，会出现灰视、管状视、短暂意识丧失、意识丧失等症状。
摘要
背景：载人航天器发射与返回、舰载机的起飞与降落，会使航天员、飞行员处在高加速度(超重)环境中，高加速度环境会对细胞、器官、动物体等产生重要影响。高加速度离心加载机是一种可以为动物个体及细胞提供高加速度力学环境的装置，在航空航天的力学生物学、组织工程研究中起基础平台作用，目前通常生物学实验室需要高加速度加载装置。
目的：结合现有离心机，设计制作大鼠高加速度离心加载机，研究大鼠在高加速度力学环境下的力学生物学响应。
方法：离心机设计制作流程：确定设计参数、选择动力源→设计离心机结构并验证→选择制作控制器→加工制作机器并调试。对大鼠进行水平和竖直放置方向的加载，每个方向设置了4 G组、8 G组、10 G组、20 G组4个小组和一个对照组，观察不同高加速度环境对大鼠身体和行走能力的影响。
结果与结论：①经过结构设计加工、配件选型和安装调试研制出高加速度加载机，设备包括机械结构与控制系统两部分；该机运行平稳，噪声低，平稳实现0-40 G的高加速度加载，并且还可实现变加速度加载；②采用该机对大鼠进行不同高加速度环境加载后，表现出身体向一侧倾斜，不能直线行走的现象，并且伴随战栗、后肢充血等症状，高加速度值越大，大鼠所受的影响越大，经过一段时间休息，大鼠身体基本可以恢复；③实验结果验证了该离心加载机可以为动物实验提供相应的高加速度环境，可以用于研究高加速度环境下动物的力学生物学响应。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-8702-9582(高子剑)

关键词: 高加速度, 离心加载, 高加速度加载机, 有限元模型, 模态分析, 共振, 振型, 大鼠, 组织构建

Abstract:

BACKGROUND: Manned spacecraft launches and returns, and shipboard aircraft takes off and landing, will expose astronauts and pilots to high acceleration environment. High acceleration environment has an important impact on cells, organs, and bodies. The high acceleration centrifuge is a device that can provide high acceleration environment for cells and animals. It plays a role of basic platform in the study on mechanics biology and tissue engineering, especially biological laboratory.
OBJECTIVE: To design a centrifuge for high acceleration loading in rats based on a centrifuge and to study the mechanobiological responses of rats in a high-acceleration environment.
METHODS: Design process of centrifuge was as follows: determine parameters and select motor → design and validate of the centrifuge → select controller → manufacture and commission machine. Rats were loaded horizontally and vertically. Four groups including 4 G group, 8 G group, 10 G group and 20 G group and one control group were set up. The effects of different high acceleration environments on the physical condition and walking ability of rats were detected.
RESULTS AND CONCLUSION: We designed and manufactured the centrifuge, and it included two parts: mechanical structure and control system. The machine run smoothly and had no noise, and could provide 0-40 G high acceleration environment and variable acceleration environment. After rats were subjected to different high acceleration loads, their bodies were inclined to one side and could not walk in a straight line, and accompanied by trembling, hindlimb congestion and other symptoms. The higher the high acceleration value, the greater the influence on rats. After a period of rest, the body could basically recover. Our test suggests that the centrifuge can provide a high acceleration environment for animal experiments, which can be used to study the mechanobiological responses of animals in high acceleration environment.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Acceleration, Biomechanics, Centrifugation, Tissue Engineering

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