中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (5): 766-773.doi: 10.3969/j.issn.2095-4344.0449

• 干细胞综述 stem cell review • 上一篇    下一篇

干细胞模型研究进展及商业化应用的现状

柯敏霞1,纪 猛2,王 皓2,洪丹萍1,吴月红1,齐念民2   

  1. 1浙江理工大学,浙江省杭州市 310018;2杭州标模生物科技有限公司,浙江省杭州市 310018
  • 修回日期:2017-09-15 出版日期:2018-02-18 发布日期:2018-02-18
  • 通讯作者: 齐念民,博士,教授,杭州标模生物科技有限公司,浙江省杭州市 310018。 并列通讯作者:吴月红,博士,副教授,浙江理工大学生命科学学院,浙江省杭州市 310018
  • 作者简介:柯敏霞,女,1992年生,浙江省台州市人,汉族,浙江理工大学生命科学学院在读硕士,主要从事干细胞方面的研究。
  • 基金资助:

    国家自然科学基金项目(31201867);浙江省自然科学基金项目(LY17C120001);企业委托研发项目(16040135-J)

Stem cell models for commercialization

Ke Min-xia1, Ji Meng2, Wang Hao2, Hong Dan-ping1, Wu Yue-hong1, Qi Nian-min2   

  1. 1Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang Province, China; 2Hangzhou Stem Cell Models Biological Technology Co., Ltd., Hangzhou 310018, Zhejiang Province, China
  • Revised:2017-09-15 Online:2018-02-18 Published:2018-02-18
  • Contact: Qi Nian-min, Ph.D., Professor, Hangzhou Stem Cell Models Biological Technology Co., Ltd., Hangzhou 310018, Zhejiang Province, China; Wu Yue-hong, Ph.D., Associate professor, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang Province, China
  • About author:Ke Min-xia, Studying for master’s degree, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31201867; the Zhejiang Provincial Natural Science Foundation of China, No. LY17C120001; the Enterprise Commissioned R & D Project, No. 16040135-J

摘要:

文章快速阅读:

文题释义:
诱导多能干细胞:
是一种体细胞直接重编程产生的多能干细胞。2006年,日本Takahashi和Yamanaka等科学家以反转录病毒为载体,在小鼠的成纤维细胞中导入4种转录因子—Oct4,Sox2,klf4,c-Myc,所获得的细胞形态和分化潜能与胚胎干细胞十分相似,称作诱导多能干细胞。诱导多能干细胞具有自我更新能力和分化成所有类型体细胞的潜能,并且不受细胞来源、道德伦理等诸多限制,为干细胞应用带来了新的机遇。Shinya Yamanaka与John Gurdon由于“发现成熟细胞可以被重编程而具有多能性”一起被授予了2012年诺贝尔生理学或医学奖。
胚胎干细胞实验:是通过欧洲替代动物实验中心(ECVAM)正式验证的胚胎和发育毒性的动物实验替代方法。经典的胚胎干细胞实验方法采用了2种细胞:分化成熟的3T3成纤维细胞和未分化的D3胚胎干细胞。基本流程是通过MTT试验检测受试物的3T3细胞毒性和D3胚胎干细胞毒性,同时检测抑制胚胎干细胞分化为心肌细胞的能力。

 

摘要
背景:
干细胞是具有自我更新能力的潜在的“永生化”细胞,是挖掘人类发育与衰老奥秘的关键,也是实现再生医学的核心。
目的:总结胚胎干细胞、成体干细胞和诱导多能干细胞的生物学特性,评述其在细胞治疗和药物筛选中的临床及商业化应用现状,并浅析干细胞产业所面临的问题和前景。
方法:应用计算机检索1995至2017年PubMed数据库、CNKI数据库中有关干细胞模型的相关文献,检索词为“干细胞,胚胎干细胞,成体干细胞,诱导多能干细胞,干细胞研究进展,干细胞治疗,药物筛选,stem cells,embryonic stem cells,adult stem cells,induced pluripotent stem cells,stem cell therapy,drug screening”。
结果与结论:①干细胞模型根据其来源分为胚胎干细胞、成体干细胞和诱导多能干细胞。不同来源的干细胞具有其独特的生物学优势;②胚胎干细胞具有发育全能性,但存在伦理道德争议;成体干细胞研究最为广泛和深入,应用最为普遍和成熟;诱导多能干细胞具有类似于胚胎干细胞的全能性,并且不受细胞来源、道德伦理等诸多限制,为干细胞应用带来了新的机遇;③干细胞在细胞治疗领域的应用技术最为成熟。目前已有多款成体干细胞商业化产品上市,用于细胞治疗;同时国际上也开展了多个利用胚胎干细胞和诱导多能干细胞进行细胞治疗的临床研究;此外,利用多能干细胞建立的细胞模型或疾病模型进行特异性药物筛选具有广泛的应用前景。

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0002-6360-152X(柯敏霞)

关键词: 干细胞, 胚胎干细胞, 成体干细胞, 诱导多能干细胞, 干细胞治疗, 药物筛选, 国家自然科学基金

Abstract:

BACKGROUND: Stem cells are the potentially immortal cells capable of self-renewal, which are essential to the mystery of human development and aging, and are also the core of research for regenerative medicine.
OBJECTIVE: To summarize the biological characteristics of embryonic stem cells, adult stem cells and induced pluripotent stem cells, to review the clinical and commercial applications in stem cell therapy and drug screening, and to analyze the problems and prospects in stem cell industry.
METHODS: We searched relevant articles about stem cell models in PubMed and CNKI databases during 1995 to 2017 on internet, and took “stem cells, embryonic stem cells, adult stem cells, induced pluripotent stem cells, stem cell therapy, drug screening” as the keywords in English and Chinese, respectively.
RESULTS AND CONCLUSION: According to the origin, stem cell models are divided into three types: embryonic stem cells, adult stem cells and induced pluripotent stem cells. Different types of stem cells have their unique biological advantages. Embryonic stem cells can generate all somatic cell types, but the application is limited by ethical disputes. As for adult stem cells, there are the most extensive and in-depth, studies as the well as the most prevalent and mature applications. Induced pluripotent stem cells have similar characteristics as embryonic stem cells, and furthermore their use avoids source restriction, moral and ethical controversies, bringing new opportunities for stem cell application. Stem cell-based cell therapy has shown successful achievement. There have been a few commercial products about adult stem cells-based cell therapy; in the meanwhile both embryonic stem cells and induced pluripotent stem cells are making their way into clinical trials. In addition, pluripotent stem cells hold great promise for the specific drug screening because they enable scientists to establish a variety of cell and disease models in vitro.

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Embryonic Stem Cells, Adult Stem Cells, Induced Pluripotent Stem Cells, Tissue Engineering

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