中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3649-3653.doi: 10.3969/j.issn.1673-8225.2012.20.009

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

张应力刺激大鼠髁突软骨细胞Ⅱ型胶原和聚集蛋白聚糖的表达*★

郑如松1，杨竹丽2，杜衍晓3，尹崇英1，贾萍萍1，袁  晓2   

  1. 1青岛大学医学院附属青岛市立医疗集团口腔科，山东省青岛市  266000；2 青岛市市立医疗集团口腔科，山东省青岛市  266071；3青岛市中心医院，山东省青岛市  266042
  • 收稿日期:2011-12-24 修回日期:2012-02-10 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 袁晓，博士，副主任医师，青岛市市立医疗集团口腔科，山东省青岛市 266071
  • 作者简介:郑如松★，男，1986年生，山东省滨州市人，汉族，青岛大学医学院在读硕士，主要从事口腔正畸学研究。
  • 基金资助:

    国家自然科学基金资助项目(31170891)。

Effect of tensile stress on type Ⅱ collagen and aggrecan expression in rat condylar chondrocytes*★

Zheng Ru-song1, Yang Zhu-li2, Du Yan-xiao3, Yin Chong-ying1, Jia Ping-ping1, Yuan Xiao2   

  1. 1Department of Stomatology, Qingdao Municipal Hospital (Group), Qingdao University Medical School, Qingdao  266000, Shandong Province, China; 2Department of Stomatology, Qingdao Municipal Hospital (Group), Qingdao  266071, Shandong Province, China; 3Qingdao Central Hospital, Qingdao  266042, Shandong Province, China
  • Received:2011-12-24 Revised:2012-02-10 Online:2012-05-13 Published:2012-05-13
  • Contact: Yuan Xiao, Doctor, Associate chief physician, Department of Stomatology, Qingdao Municipal Hospital (Group), Qingdao 266071, Shandong Province, China
  • About author: Yuan Xiao, Doctor, Associate chief physician, Department of Stomatology, Qingdao Municipal Hospital (Group), Qingdao 266071, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31170891*

PDF

532

被引次数

3

摘要：

背景：研究表明软骨细胞外基质合成的变化反映了外力对于颞下颌关节的影响和机体对于外力的适应性。
目的：观察周期性张应力对髁突软骨细胞主要细胞外基质合成的影响。
方法：采用FX-5000T应力加载系统对第3代大鼠髁突软骨细胞分别施加0，1，6，12和24 h的周期性张应力，应力刺激强度为10% 1 Hz。加力完成后收集加力细胞，提取总RNA反转录成cDNA，应用RT-PCR技术检测软骨细胞主要细胞外基质Ⅱ型胶原和聚集蛋白聚糖的表达变化情况。
结果与结论：与对照组(0 h组)相比，加力   6 h时Ⅱ型胶原和聚集蛋白聚糖表达均显著增加(P < 0.05)；加力12 h时Ⅱ型胶原和聚集蛋白聚糖表达均开始下降；当加力至24 h时二者表达量均显著降低(P < 0.05)。结果表明：周期性张应力可以影响髁突软骨细胞主要细胞外基质的合成,随加力时间的延长基质合成逐渐增强；进一步延长加力时间,基质的合成受到明显抑制。
 

关键词: 张应力, 功能矫形, 髁状突软骨, Ⅱ型胶原, 聚集蛋白聚糖

Abstract:

BACKGROUND: Changes in extracellular of chondrocyte can reflect influence of external force on temporomandibular joint and adaptability of body to external force.
OBJECTIVE: To study the effect of cyclic tensile stress on main extracellular matrix of condylar chondrocyte.
METHODS: The cyclic tensile stress was exposed to the third passage condylar chondrocyte for 0, 1, 6, 12 and 24 hours, respectively, using a Flexcell Strain Unit-5000T system (10% surface elongation, 6 cycles/min). After mechanical loading, total RNA was extracted from the cells harvested from Six-well BioFlex flexible cell Petri Dish, reverse transcribed, and reverse trabscription-PCR was performed to quantify mRNA levels for type Ⅱ collagen and aggrecan.
RESULTS AND CONCLUSION: Compared with the control group (0 hour group), both type Ⅱ collagen and aggrecan mRNA expression was significantly increased after loading for 6 hours (P < 0.05), but began to decrease since 12 hours, and significantly decreased at 24 hours (P < 0.05). Results showed that cyclical tensile stress stimuli can affect the synthesis of main extracellular matrix of condylar cartilage, i.e. the synthesis was gradually enhanced with prolonged stimulation duration, but significantly inhibited in response to further stress stimuli.

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