中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1463-1466.doi: 10.3969/j.issn.1673-8225.2012.08.030

• 生物材料综述 biomaterial review • 上一篇    下一篇

脂质体在基因治疗中的应用研究及进展***★

江献芳，周  诺   

  1. 广西医科大学附属口腔医院口腔颌面外科，广西壮族自治区南宁市 530021
  • 收稿日期:2011-09-20 修回日期:2011-10-15 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 周诺，博士，博士生导师，主任医师，教授，广西医科大学附属口腔医院，广西壮族自治区南宁市 530021
  • 作者简介:江献芳★，女，1986年生，广西壮族自治区南宁市人，汉族，在读硕士，医师，主要从事颅颌面畸形外科治疗、颌面部肿瘤的基础与临床研究。jiangmubo@ 163.com
  • 基金资助:

    国家自然科学基金资助项目(30760273、30960421)；广西壮族自治区自然科学基金资助项目(桂科自0991013Z)。

Research and application of liposomes in gene therapy 

Jiang Xian-fang, Zhou Nuo   

  1. Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-20 Revised:2011-10-15 Online:2012-02-19 Published:2012-02-19
  • Contact: Zhou Nuo, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Jiang Xian-fang★, Studying for master’s degree, Physician, Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China jiangmubo@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30760273*, 30960421*;the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0991013Z*

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摘要：

背景：脂质体是常用的安全的基因载体，然而由于其本身组成及结构的原因，应用和发展受到了限制，其转染效率和治疗效果成为基因治疗研究中最为重要的参考因素。
目的：回顾脂质体在基因治疗中的应用以及研究进展。
方法：由第一作者计算机检索1995/2011中国期刊全文数据库、CALIS全国医学文献/服务系统以及康健循证医学知识仓库，中英文检索词分别为“脂质体、基因、转染”和“liposome、gene、transfection”。
结果与结论：脂质体作为基因载体较病毒载体具有安全性高，免疫原性小，毒性小，容易制备等优点已成功应用于很多体外及动物体内实验，但由于其转染效率低，靶向性低等缺点使其发展受到了很大限制，所以目前脂质体在基因治疗中的研究热点在于提高脂质体的转染效率，在靶细胞和靶器官达到治疗浓度才能有更好的治疗效果。

关键词: 脂质体, 基因, 转染, 靶细胞, 靶器官, 治疗浓度

Abstract:

BACKGROUND: Liposome is a safe and frequently-used genonic vector. But the composition and structure of liposome limit its application and development. Transfection efficiency and treatment effect of the liposome become the most important reference factors in the research of gene therapy.
OBJECTIVE: To review the application and research progress of liposome in gene therapy.
METHODS: CNKI database, CALIS database and KANGJIAN database were searched for articles from 1995 to 2011 with the key words of “liposome, gene, transfection” in Chinese and English.
RESULTS AND CONCLUSION: Liposome as the genonic vector has many advantages in higher security, lower immunogenicity, less toxicity and easy preparation as compared with viral vector. Therefore, it has been successfully applied in many animal experiments in vivo and in vitro. The application of liposome is restricted by lower transfection efficiency and target activity. So the hot spot of study in gene therapy involves in liposome is to improve the transfection efficiency. The better therapeutic effect is obtained by reaching the treatment concentration in target cells or organ.

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