中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8301-8304.doi: 10.3969/j.issn.1673-8225.2011.44.033

• 移植与基因 transplantation and gene • 上一篇    下一篇

造血干细胞移植56例人类白细胞抗原基因和单倍型分析

刘  川1，2，万  华3，邹叶青1，李  剑1，李国良4，傅颖媛2   

  1. 1南昌大学第二附属医院医学分子实验室，江西省南昌市  330006
    2南昌大学医学院免疫教研室，江西省南昌市  330006
    3南昌市第一医院检验科，江西省南昌市  330008
    4江西省血液中心组织配型实验室，江西省南昌市  330077
  • 收稿日期:2011-04-08 修回日期:2011-06-23 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 傅颖媛，硕士生导师，教授。南昌大学医学院免疫教研室，江西省南昌市 330006 hqfyy@126.com
  • 作者简介:刘川★，男，1979年生，江西省丰城市人，汉族，南昌大学医学院在读硕士，主要从事分子生物学和组织配型的研究。 lc61116@163. com

Human leukocyte antigen allele and haplotype in 56 cases of hematopoietic stem cell transplantation

Liu Chuan1,2, Wan Hua3, Zou Ye-qing1, Li Jian1, Li Guo-liang4, Fu Ying-yuan2   

  1. 1Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang   330006, Jiangxi Province, China
    2Immunization Department, Medical College of Nanchang University, Nanchang   330006, Jiangxi Province, China
    3Laboratory of the First Hospital of Nanchang, Nanchang  330008, Jiangxi Province, China
    4tissue Matching Laboratory of Jiangxi Province Blood Center, Nanchang 330077, Jiangxi Province, China
  • Received:2011-04-08 Revised:2011-06-23 Online:2011-10-29 Published:2011-10-29
  • Contact: Fu Ying-yuan,Professor, Master’s supervisor, Immunization Department, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China hqfyy@126.com
  • About author:Liu Chuan★, Studying for master’s degree, Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; Immunization Department, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China lc61116@163.com

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摘要：

背景：收集56例欲行造血干细胞移植的供受者，均为无血缘关系的江西省汉族人群。了解个体的人类白细胞抗原基因型和单倍型。
目的：分析56例造血干细胞移植供受者的人类白细胞抗原基因频率，单倍型频率。
方法：收集56例欲行造血干细胞移植的供受者，均无血缘关系的江西省汉族人群。应用PCR-SSP的方法进行人类白细胞抗原(HLA)-A、B、DRB1基因分型，计算出HLA-A、B、DRB1各位点的基因频率和单倍型频率。
结果与结论：56例供受者测出HLA-A位点等位基因8种，HLA-B位点等位基因19种，HLA-DRB1位点等位基因13种，呈现出丰富的基因多态性。56例供受者两位点共224条等位基因中，A﹡02-B﹡46、A﹡11-B﹡40、B﹡46-DRB1﹡09单倍型的频率高于0.10。有10种A-B单倍型，4种B-DRB1单倍型呈现出显著的连锁不平衡。提示江西省汉族人群人类白细胞抗原基因具有较丰富的基因多态性。

关键词: 造血干细胞移植, 人类白细胞抗原, 单倍型, 基因, 遗传

Abstract:

BACKGROUND: It has great significance to understand individual human leucocyte antigen allele (HLA) haplotype and genotype.
OBJECTIVE: To analyze HLA allele frequency, haplotype frequency and characteristics in 56 cases of hematopoietic stem cell transplantation.
METHODS: HLA-A, B, DRB1 antigen was subjected to genotyping using PCR-SSP technique. The allele and haplotype frequencies of HLA-A, B, DRB1 were calculated.
RESULTS AND CONCLUSION: A total of 8 HLA-A, 19 HLA-B and 13 HLA-DRB1 alleles were detected among 56 cases, showing a rich polymorphism. Among 224 haplotypes, A﹡02-B﹡46, A﹡11-B﹡40, B﹡46-DRB1﹡09 were informative with frequency higher than 0.10. A total of 10 HLA-A-B haplotypes and 4 HLA-B-DRB1 haplotypes were in strongest linkage disequilibrium. Results showed that HLA alleles have richer polymorphisms with obvious geographic genetic characteristics.

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