中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7429-7432.doi: 10.3969/j.issn.1673-8225.2011.40.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

乙烯雌酚可诱导骨髓基质干细胞向成骨细胞分化

张长海1，张宪成1，胡  萌1，薛政民1，周晓鹏1，李翠云2   

  1. 1济宁市第二人民医院脊柱外科，山东省济宁市   272000
    2济宁市肿瘤医院放疗科，山东省济宁市   272000
  • 收稿日期:2011-01-25 修回日期:2011-03-23 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 胡萌，硕士，医师，济宁市第二人民医院脊柱外科，山东省济宁市272000
  • 作者简介:张长海，男，1968年生，山东省鱼台县人，汉族，1993年济宁医学院毕业，副主任医师，主要从事脊柱及创伤骨科疾病的临床治疗与基础研究。

Diethylstilbestrol can induce bone marrow stromal cells to differentiate into osteoblasts

Zhang Chang-hai1, Zhang Xian-cheng1, Hu Meng1, Xue Zheng-min1, Zhou Xiao-peng1, Li Cui-yun2   

  1. 1Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining  272000, Shandong Province, China
    2Department of Radiation Oncology, Jining Tumor Hospital, Jining  272000, Shandong Province, China
  • Received:2011-01-25 Revised:2011-03-23 Online:2011-10-01 Published:2011-10-01
  • Contact: Hu Meng, Master, Physician, Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining 272000, Shandong Province, China
  • About author:Zhang Chang-hai, Associate chief physician, Department of Spinal Surgery, Second People’s Hospital of Jining City, Jining 272000, Shandong Province, China Humeng810423@163.com

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被引次数

1

摘要：

背景：关于雌激素对骨髓基质干细胞作用的报道尚不多。
目的：观察乙烯雌酚对兔骨髓基质干细胞成骨分化的影响。
方法：体外培养骨髓基质干细胞，用0，10-7，10-6，10-5 mol/L浓度乙烯雌酚干预，并设地塞米松10-8 mol/L、β-甘油磷酸钠10 mmol/L、维生素C 50 mg/L为阳性对照。
结果与结论：乙烯雌酚干预培养24，48，72 h，10-6 mol/L组显著促进了骨髓基质干细胞增殖(P < 0.01)。干预48 h 10-5 mol/L组显著抑制细胞增殖，干预72 h 10-7 mol/L组显著抑制细胞增殖(P < 0.01)。10-7 mol/L组乙烯雌酚干预25 d后开始出现钙化结节；10-7，10-6 mol/L组干预14，21 d碱性磷酸酶活性显著增加。证实乙烯雌酚能促进兔骨髓基质干细胞的成骨分化。

关键词: 乙烯雌酚, 骨髓基质干细胞, 成骨分化, 增殖, 碱性磷酸酶

Abstract:

BACKGROUND: There are fewer reports about estrogen effects on bone marrow stromal cells (BMSCs).
OBJECTIVE: To study the effect of diethylstilbestrol on the osteogenic differentiation of rabbit BMSCs.
METHODS: Rabbit BMSCs cultured in vitro were intervened with 0, 10-7, 10-6, 10-5 mol/L diethylstilbestrol, and BMSCs cultured with dexamethasone 10-8 mol/L, β-sodium glycerophosphate 10 mmol/L, and vitamin C 50 mg/L were used as positive controls.
RESULTS AND CONCLUSION: 10-6 mol/L diethylstilbestrol significantly improved the proliferative ability of BMSCs at 24, 48, and 72 hours after intervention (P < 0.01). 10-5 mol/L diethylstilbestrol significantly inhibited the proliferation of BMSCs at 48 hours after intervention as well as 10-7 mol/L diethylstilbestrol at 72 hours (P < 0.01). Mineralized nodular structures formed at 25 days after intervention with 10-7 mol/L diethylstilbestrol. Alkaline phosphatase activities were remarkably increased at 14 and 21 days after intervention with 10-7, 10-6 mol/L diethylstilbestrol. It has been proved that diethylstilbestrol has an enhancing effect on the osteogenic differentiation of rabbit BMSCs.

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