中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 261-264.doi: 10.3969/j.issn.1673-8225.2011.02.017

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

单核细胞趋化蛋白1和骨形成蛋白7在病理性瘢痕中的表达

李永涛1，2 ，王喜梅1 ，刘林嶓1， 张建文1， 袁德品1，2   

  1. 1郑州大学第一附属医院整形外科，河南省郑州市450052
    2河南省高等学校临床医学重点学科开放实验室病理学实验室，河南省郑州市  450052
  • 收稿日期:2010-09-08 修回日期:2010-12-09 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 王喜梅，博士，主任医师，硕士生导师，郑州大学第一附属医院整形外科，河南省郑州市450052 wangximei228@gmail.com
  • 作者简介:李永涛★，男，1983年生，山东省菏泽市人，郑州大学第一附属医院整形外科在读硕士，主要从事病理性瘢痕的基础与临床研究。 liyongtao830123@163.com

Expression of monocyte ehemoattraetant protein-1 and bone morphogenic protein-7 in pathologic scars  

Li Yong-tao1, 2, Wang Xi-mei1, Liu Lin-bo1, Zhang Jian-wen1, Yuan De-pin1, 2   

  1. 1Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou  450052, Henan Province, China
    2Open Laboratory of Phytopathology, Key Discipline of Clinical Medical Sciences, Institution of Henan Province High Learning, Zhengzhou  450052, Henan Province, China
  • Received:2010-09-08 Revised:2010-12-09 Online:2011-01-08 Published:2011-01-08
  • Contact: Wang Xi-mei, Doctor, Chief physician, Master’s supervisor, Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China wangximei228@ gmail.com
  • About author:Li Yong-tao★, Studying for Master’s degree, Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Phytopathology, Key Discipline of Clinical Medical Sciences, Institution of Henan Province High Learning, Zhengzhou 450052, Henan Province, China liyongtao830123@ 163.com

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382

被引次数

9

摘要：

背景：单核细胞趋化蛋白1是新近明确的对单核/巨噬细胞有趋化和激活双重作用的趋化因子，骨形成蛋白7作为一种新发现的纤维化负性调节因子逐渐成为抗组织纤维化治疗的研究热点，但两者对病理性瘢痕形成中组织纤维化作用的研究至今鲜有报道。
目的：研究单核细胞趋化蛋白1，骨形成蛋白7在病理性瘢痕中的表达水平。
方法：采用免疫组织化学方法检测单核细胞趋化蛋白1、骨形成蛋白7在25例瘢痕疙瘩、30例增生性瘢痕、24例非病理瘢痕和20例正常皮肤组织中的表达水平。所有标本均来自2008-07/2010-01郑州大学第一附属医院整形外科住院患者，且均无皮肤疾病、结缔组织病、传染病、恶性肿瘤和其他重要脏器疾病，术前无射线治疗、激光治疗及免疫治疗史，其中所取瘢痕组织来自于临床诊断明确的瘢痕患者。
结果与结论：单核细胞趋化蛋白1在瘢痕疙瘩、增生性瘢痕中的阳性表达率均高于非病理性瘢痕与正常皮肤组织(P < 0.05)，骨形成蛋白7阳性表达率均降低(P < 0.05)，两者阳性表达率在病理性瘢痕(瘢痕疙瘩和增生性瘢痕)中呈明显负相关(r = -0.639，P < 0.01)。结果显示，在病理性瘢痕的形成过程中单核细胞趋化蛋白1表达上调，而骨形成蛋白7表达下调。 

关键词: 单核细胞趋化蛋白1, 骨形成蛋白7, 病理性瘢痕, 免疫组织化学, 组织构建

Abstract:

BACKGROUND: Monocyte ehemoattraetant protein-1 (MCP-1) has been shown chemotaxis and activation effect on mononuclear/macrophage. As a newly found negative-regulatory factor, bone morphogenic protein-7 (BMP-7) has aroused increasing attention in the treatment of tissue fibrosis. However, the effects of MCP-1 and BMP-7 on tissue fibrosis during pathologic scars remain poorly understood. 
OBJECTIVE: To investigate the expression of MCP-1 and BMP-7 in pathologic scars.
METHODS: SP immunohistochemical method was used to detect the expressions of MCP-1 and BMP-7 in 25 cases of keloid, 30 cases of hypertrophic scars, 24 cases of non-pathologic scar and 20 cases of normal skin tissues. All specimens obtained from plastic surgery patients that had no skin disease, connective disease, infectious disease, malignant tumor and other important viscera diseases, no radiation therapy, laser therapy and immunotherapy before surgery in the First Affiliated Hospital of Zhengzhou University. Meanwhile, the scar tissues were taken from the patients who were diagnosed clearly by the clinic, and they were confirmed by pathology.
RESULTS AND CONCLUSION: The positive rates of MCP-1 in keloid group was higher than that of the hypertrophic scar and normal skin groups (P < 0.05), but the positive rates of BMP-7 was decreased (P < 0.05). There was a negative correlation between MCP-1 and BMP-7 expression in pathologic scars (keloid and hypertrophic scars) (r=-0.639, P < 0.01). Results demonstrated that MCP-1 expression up regulated and BMP-7 expression down regulated during development of pathologic scars.

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