中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (50): 9333-.doi: 10.3969/j.issn.1673-8225.2010.50.006

• 软骨组织构建 • 上一篇    下一篇

病理性瘢痕中哺乳动物雷帕霉素靶蛋白/核糖体40S小亚基S6蛋白激酶的表达

袁德品1，2，牛扶幼1，陈旻静1，王喜梅1，李永涛1，2

 
  

  1. 1郑州大学第一附属医院整形外科，河南省郑州市450052；2河南省高等学校临床医学重点学科开放实验室，河南省郑州市 450052
  • 出版日期:2010-12-10 发布日期:2010-12-10
  • 通讯作者: 牛扶幼，女，教授，主任医师，硕士生导师，主要从事瘢痕形成机制及临床治疗。郑州大学第一附属医院整形外科，河南省郑州市 450052 niufuyou@zzu.edu.cn
  • 作者简介:袁德品★，男，1984年生，河南省商丘市人，汉族，郑州大学医学院在读硕士，主要从事瘢痕形成机制及临床治疗方面的研究。 yuandepin@126.com 并列第一作者：陈旻静，女，1967年生，河南省洛阳市人，教授，主任医师，硕士生导师，1990年河南医科大学毕业，主要从事瘢痕形成机制及临床治疗。chenmj67@yahoo.com.cn

Expression of mammalian target of rapamycin/P70S6K in pathologic scars
 

Yuan De-pin 1,2, Niu Fu-you1, Chen Min-jing1, Wang Xi-mei 1, Li Yong-tao 1,2

 
  

  1. 1 Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2 Open Laboratory of Key Discipline of Clinical Medical Sciences, Institutions of Henan Province High Learning, Zhengzhou 450052, Henan Province, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Niu Fu-you, Professor, Chief physician, Master’s supervisor, Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China niufuyou@zzu.deu.cn
  • About author:Yuan De-pin★, Studying for master’s degree, Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Key Discipline of Clinical Medical Sciences, Institutions of Henan Province High Learning, Zhengzhou 450052, Henan Province, China yuandepin@126.com Chen Min-jing, Professor, Chief physician, Master’s supervisor, Department of Plastic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China chenmj67@yahoo.com.cn Yuan De-pin and Chen Min-jing contributed equally to this paper

PDF

348

被引次数

9

摘要：

背景：近年研究表明哺乳动物雷帕霉素靶蛋白/核糖体40S小亚基S6蛋白激酶(mammalian target of rapamycin/P70S6K，mTOR/P70S6K)信号通路在某些肿瘤形成过程中具有一定作用。病理性瘢痕特别是瘢痕疙瘩具有某些肿瘤的性质，因此该信号通路在病理性瘢痕形成中可能具有重要作用。
目的：观察mTOR/P70S6K信号通路在病理性瘢痕中的的作用。
方法：采用免疫组织化学SP法检测磷酸化mTOR/P70S6K(p-mTOR和p-P70S6K)在瘢痕疙瘩、增生性瘢痕、非病理性瘢痕及正常皮肤组织中的表达水平。实验所取瘢痕组织来自临床上诊断明确的瘢痕患者。
结果：瘢痕疙瘩和增生性瘢痕组织中p-mTOR和p-P70S6K阳性表达率高于非病理性瘢痕及正常皮肤组(P < 0.05)。p-mTOR和p-P70S6K表达呈正相关(r = 0.482，P < 0.05)。结果提示mTOR/P70S6K信号通路的激活参与了病理性瘢痕的形成过程，且mTOR和P70S6K之间具有协同作用。

关键词: 哺乳动物雷帕霉素靶蛋白, 核糖体40S小亚基S6蛋白激酶, 瘢痕疙瘩, 增生性瘢痕, 组织构建

Abstract:

BACKGROUND: In recent years, related research has shown that mammalian target of rapamycin/P70S6K (mTOR/P70S6K) signaling pathway has a role in tumor formation. Pathologic scar, especially the keloid scar, with the nature of some tumors, therefore mTOR/P70S6K signal pathway may be of great significance in the formation of pathological scars.
OBJECTIVE: To investigate the role of mTOR/P70S6K signalling pathway in pathologic scars.
METHODS: SP immunohistochemical method was used to detect the expressions of phosphated mTOR (p-mTOR) and phosphated PTOS6K (p-P70S6K) p-mTOR and p-P70S6K in keloid, hypertrophic scar, non-pathologic scar and normal skin tissues. All the scar tissues were taken from the patients who were diagnosed clearly by the clinic, and they were confirmed by pathology.
RESULTS AND CONCLUSION: The positive rates of p-mTOR and p-P70S6K in keloid and hypertrophic scars were higher compared with the two control groups (P < 0.05). There was a highly positive correlation between p-mTOR and p-P70S6K expression in pathologic scars (r=0.482, P < 0.05). The activation of mTOR/P70S6K signalling pathway might be involved in the formation of pathologic scar, and the factors of it may cooperate to promote the progress of pathologic scars.

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