中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8531-8534.doi: 10.3969/j.issn.1673-8225.2010.45.040

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

儿童骨髓增生异常综合征造血前体细胞增殖与白细胞介素15的影响

程涵蓉1，叶中绿2，陈铭珍2   

  1. 1暨南大学第二临床医学院，深圳市人民医院儿科, 广东省深圳市 518020；2广东医学院附属医院儿科，广东省湛江市 524023　
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 陈铭珍，教授，主任医师，广东医学院附属医院儿科，广东省湛江市 524023
  • 作者简介:程涵蓉★，女，1977年生，广东省深圳市人，汉族，2004年广东医学院毕业，硕士，主治医师，主要从事小儿血液方面的研究。 chr621@163.com
  • 基金资助:

    湛江市科技攻关项目（020113），IL-15对儿童骨髓增生异常综合征造血干细胞增殖分化及凋亡的影响研究。

Effects of interleukin-15 on proliferation of children’s myelodysplastic syndrome hematopoietic stem/progenitor cells

Cheng Han-rong1, Ye Zhong-lü2, Chen Ming-zhen2   

  1. 1  Department of Pediatrics, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen  518020, Guangdong Province, China; 2 Department of Pediatrics,  Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Chen Ming-zhen, Proferssor, Chief physician, Department of Pediatrics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China.
  • About author:Cheng Han-rong★, Master, Attending physician, Department of Pediatrics, Shenzhen People’s Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, China chr621@163.com
  • Supported by:

     the Science and Technology Key Program of Zhanjiang City, No. 020113* 

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摘要：

背景：白细胞介素15是一种重要的骨髓生长调节因子，可能具有刺激骨髓增生异常综征 CD34+细胞增殖的作用。
目的：观察白细胞介素15对儿童骨髓增生异常综合征造血细胞增殖的影响。
方法：采用细胞计数、MTT抑制实验检测0，10，20，50，100，200 µg/L白细胞介素15对18例1~14岁儿童骨髓增生异常综合征患者 CD34+细胞增殖的影响，找出白细胞介素15作用的峰浓度。以白细胞介素15达到峰值的浓度为实验组，不加白细胞介素15的为对照组，培养12 d以了解细胞最佳增殖时间。
结果与结论：体外培养的骨髓CD34+细胞在培养第8天生长状况最好，并且随着白细胞介素15 质量浓度的增加，其活细胞数也随着增加，当白细胞介素15为100 µg/L时，其增殖状况最好；当白细胞介素15为200 µg/L时，其增殖状况反而欠佳。在培养的第8天，白细胞介素15对骨髓增生异常综合征患者骨髓CD34+细胞抑制率最低，当其浓度在0~100 µg/L范围内，随质量浓度增加，其抑制作用递减，而当其质量浓度>100 µg/L其抑制作用有所增强。说明白细胞介素15呈时间和剂量依赖性地促进骨髓增生异常综合征患者骨髓CD34+造血干/祖细胞增殖。

关键词: 白细胞介素15, 骨髓增生异常综合征, 造血干细胞, 增殖, CD34+细胞

Abstract:

BACKGROUND: Interleukin (IL)-15 is a member of hematopoietic cytokine superfamily. It may stimulate the proliferation of the myelodysplastic syndrome (MDS) hematopoietic stem/progenitor cells.
OBJECTIVE: To investigate the effects of IL-15 on proliferation of hematopoietic cells in MDS child.
METHODS: Cell-counting, MTT-inhibitory test were used to examine the inhibitory effect of IL-15 (0, 10, 20, 50, 100, 200 µg/L) on the proliferation of MDS CD34+ cells in 18 children aged 1-14 years to find out the peak concentration of IL-15. IL-15 at peak concentration served as experimental group, whereas those without IL-15 as control group. 12-day culture was done to understand optimal proliferation time of cells.
RESULTS AND CONCLUSION: At day 8, CD34+cells cultured in vitro grew well. With increased IL-15 concentration, living cells number increased as well. When IL-15 was 100 µg/L, its proliferation status was the best, but when IL-15 was 200 µg/L, its proliferation status was below the mark. At day 8, the inhibitory rate of IL-15 on CD34+ cells was the lowest. When IL-15’s concentration was between 0 and 100 µg/L. With increased IL-15’s concentration, the inhibition decreased progressively. When IL-15 was above 100 µg/L, the inhibition reinforced on the contrary. These indicate that IL-15 can accelerate MDS bone marrow CD34+ hematopoietic stem/progenitor cell proliferation in a dose-and time-dependent manner

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