中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8503-8506.doi: 10.3969/j.issn.1673-8225.2010.45.034

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

中药诱导干细胞分化为心肌细胞的效应　

王东进1，施广飞2，姜  宏3，余洪松1   

  1. 1南京中医药大学第一临床医学院中西医结合鼓楼医院，江苏省南京市  210008；2南京大学医学院附属鼓楼医院心脏科，江苏省南京市  210008；3南京中医药大学第一临床医学院，江苏省南京市  210046
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 施广飞，博士，主任医师，南京大学医学院附属鼓楼医院心脏科，江苏省南京市 210008 njglxzsgf@yahoo.com.cn
  • 作者简介:王东进☆，男，1963年生，吉林省长春市人，汉族，南京中医药大学毕业，博士，主任医师，主要从事心血管疾病方面的研究。 gldjw@163.com
  • 基金资助:

    江苏省卫生厅科技项目(H200220)；南京市医学重点科技发展项目(ZKX0206)。

Effects of traditional Chinese medicine in the induction of stem cell differentiation into cardiomyocytes

Wang Dong-jin1, Shi Guang-fei2, Jiang Hong3, Yu Hong-song1   

  1. 1 Drum Tower Hospital, Department of Integrated Traditional and Western Medicine, First Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing  210008, Jiangsu Province, China; 2 Department of Cardiology, Drum Tower Hospital of Nanjing University Medical School, Nanjing  210008, Jiangsu Province, China; 3 First Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, Jiangsu Province, China　 
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Shi Guang-fei, Doctor, Chief physician, Department of Cardiology, Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China njglxzsgf@yahoo.com.cn
  • About author:Wang Dong-jin☆, Doctor, Chief physician, Drum Tower Hospital, Department of Integrated Traditional and Western Medicine, First Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210008, Jiangsu Province, China gldjw@163.com
  • Supported by:

    the Science and Technology Projects of Health Department of Jiangsu Province, No. H200220*; the Key Medical Science and Technology Development Projects of Nanjing City, No. ZKX0206*

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摘要：

背景：经典干细胞诱导剂由于具有细胞毒性而仅仅应用在干细胞体外实验中，这对进一步提高干细胞移植疗效提出了挑战。越来越多的课题组证实，中药作为全新的干细胞诱导剂可以诱导胚胎干细胞和骨髓间充质干细胞分化为心肌细胞。课题组长期从事心肌干细胞研究，利用从中药川芎中提取的生物碱川芎嗪，已成功在体外诱导心肌干细胞分化为心肌细胞。
目的：总结中药干细胞诱导剂的整体研究进展情况，为进一步提高心血管疾病干细胞移植治疗过程中干细胞诱导分化为心肌细胞的效率奠定基础。
方法：以”traditional Chinese medicine，stem cells，cardiomyocytes”为英文关键词，以“中药，中草药，干细胞，心肌细胞”为中文关键词，检索2005/2009西文生物医学期刊文献数据库(www.kjmed.com.cn)、中国全文期刊数据库、中国博士学位论文全文数据库及中国优秀硕士学位论文全文数据库(www.cnki.net)有关中药诱导干细胞分化为心肌细胞的文献，排除重复性研究。检索文献类型包括研究论著、学位论文及综述。
结果与结论：共保留30篇文献进一步分析，中文14篇，英文16篇。结果显示中药作为一种全新的干细胞诱导剂，不仅能够有效诱导干细胞分化为心肌细胞，而且具有无毒副作用的特点，因此很有希望应用于临床干细胞治疗中。中药在诱导干细胞分化为心肌细胞方面的研究较新，随着研究深入，将会有越来越多的中药诱导剂被发现。

关键词: 中药, 干细胞移植, 心肌细胞, 干细胞诱导剂, 综述

Abstract:

BACKGROUND: Classical stem cell inducer due to its cytotoxicity only has been utilized in stem cell vitro experiment, which provides a challenge for elevating the therapeutic effect of stem cell transplantation. More and more studies have verified that traditional Chinese medicine as a new stem cell inducer can induce the differentiation of embryonic stem cells and bone marrow mesenchymal stem cells (BMSCs) into cardiomyocytes. This study group engaged in myocardial stem cell research for a long term. The alkaloid ligustrazine extracted from Szechwan Lovage Rhizome has successfully induced the differentiation of cardiac stem cells into cardiomyocytes in vitro.
OBJECTIVE: To summarize the advance in traditional Chinese medicine stem cell inducer and to provide a basis for the efficiency of stem cell differentiation into cardiomyocytes during cardiovascular diseases stem cell transplantation.
METHODS: Key words were “traditional Chinese medicine, stem cells, cardiomyocytes”. Foreign Medical Journal Service (www.kjmed.com.cn), Chinese journal full-text database, China Doctor Dissertations Full-text Database and China National Knowledge Infrastructure were searched for articles on the differentiation of stem cells into cardiomyocytes induced by traditional Chinese medicine published from 2005 to 2009. The repetitive studies were excluded. The retrieved literatures contained original articles, thesis and reviews.
RESULTS AND CONCLUSION: A total of 30 articles were included for this review, including 14 Chinese articles and 16 English articles. Results exhibited that traditional Chinese medicine as a new stem cell inducer not only effectively induced stem cell differentiation into cardiomyocytes, but also was characterized by no side or adverse effects. Therefore, it is hopeful to use in clinical stem cell therapy. It is new for traditional Chinese medicine to induce stem cell differentiation into cardiomyocytes. With deep research, more and more traditional Chinese medicine inducer will be found.

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