中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1915-1918.doi: 10.3969/j.issn.1673-8225.2010.11.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

过氧化物酶Ⅱ对体外培养人退变腰椎间盘髓核细胞的抑制作用

刘玉林，周初松，陈  仲，罗  平，林荔军   

  1. 南方医科大学附属珠江医院骨科，广东省广州市510282
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 周初松，主任医师，副教授，南方医科大学附属珠江医院骨科，广东省广州市 510282 zcsmd@yahoo.com.cn
  • 作者简介:刘玉林，男，1980年生，河北省保定市人，汉族，南方医科大学在读硕士，主要从事脊柱损伤机制的研究。 liuyulinhp20068888@126.com

Inhibition of peroxiredoxin Ⅱ on human intervertebral disc cells cultured in vitro

Liu Yu-lin, Zhou Chu-song, Chen Zhong, Luo Ping, Lin Li-jun   

  1. Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou  510280, Guangdong Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Zhou Chu-song, Chief physician, Associate professor, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China zcsmd@yahoo.com.cn
  • About author:Liu Yu-lin, Studying for master’s degree, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China liuyulinhp200068888@126.com

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441

被引次数

2

摘要：

背景：椎间盘退变可导致髓核细胞数量的减少，过氧化物酶Ⅱ可参与细胞的抗氧化损伤、细胞分裂、分化、信号转导和凋亡等过程的调控。过氧化物酶Ⅱ对椎间盘退变有促进作用，但其机制仍不清楚。
目的：观察过氧化物酶Ⅱ对体外培养的人退变腰椎间盘髓核细胞的活性和Ⅱ型胶原合成的影响。
方法：体外培养人退变腰椎间盘髓核细胞，设置对照组及过氧化物酶Ⅱ10，100和1 000 ng/L组。对照组不含氧化物酶Ⅱ，其他3组加入相应剂量的氧化物酶Ⅱ。应用免疫组化法鉴定髓核细胞，并采用cck-8试剂盒检测细胞增殖情况，于加过氧化物酶Ⅱ后第3和7天分别取对照组及各组细胞上清液，采用双抗体夹心酶联免疫吸附实验测定Ⅱ型胶原表达情况。
结果与结论：在体外培养的人退变腰椎间盘髓核细胞，随加入的过氧化物酶Ⅱ质量浓度的增加，椎间盘髓核细胞数量和Ⅱ型胶原合成逐渐减少(P < 0.01)。提示过氧化物酶Ⅱ对椎间盘髓核细胞的数量和Ⅱ型胶原合成有明显的抑制作用，并呈剂量依赖关系。以此推测过氧化物酶Ⅱ对髓核细胞的抑制作用可能是导致椎间盘退变的一种促发因素。

关键词: 过氧化物酶Ⅱ, Ⅱ型胶原, 椎间盘细胞, 髓核, 椎间盘退变, 组织因子

Abstract:

BACKGROUND: Intervertebral disc degeneration can reduce nucleus pulposus cells, and peroxiredoxin Ⅱ involved in the regulation of resist oxidation damage, cell division, differentiation, signal transduction and apoptosis. Peroxiredoxin Ⅱ has promotive effect on intervertebral disc degeneration, whereas the mechanism remains poorly understood.    
OBJECTIVE: To observe the effects of peroxiredoxin Ⅱ on human intevertebral disc cells activity and type Ⅱ collagen synthesis in vitro.
METHODS: Human degenerated human lumbar disc cells were cultured in vitro, and assigned into the control and peroxidase Ⅱ groups. Peroxidase Ⅱ with doses of 10, 100 and 1 000 ng/L were added into the peroxidase Ⅱ groups. The cells were identified by immunohistochemical staining, and the cell proliferation was detected using cck-8 kit. Cell supernatant was collected at days 3 and 7 after operation, and the expression of type Ⅱ collagen was measured by double-antibody sandwich enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: In vitro cultured human degenerative lumbar intervertebral disc nucleus pulposus cells by adding peroxidase increased with the dose-Ⅱ, the disc nucleus pulposus cells of the volume and type Ⅱ collagen synthesis gradually reduced (P < 0.01). Tips peroxidase Ⅱ on the intervertebral disc nucleus pulposus cells, the number and type Ⅱ collagen synthesis significantly inhibited in a dose-dependent manner. Thus speculated that peroxidase Ⅱ on the nucleus pulposus cells in vitro may lead to disc degeneration as a precipitating factor.

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