中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (12): 1890-1894.doi: 10.12307/2024.032

• 骨与关节图像与影像 bone and joint imaging • 上一篇    下一篇

下颈椎椎弓根的显微形态学特征

李  琨1，2，张少杰1，2，史  君3，王  建1 ，刘亚楠4 ，朵  岚4 ，杨  洋4 ，郝韵腾4 ，李志军1，2，王  星1，2   

  1. 内蒙古医科大学基础医学院，1解剖学教研室，2数字医学中心，3生理学教研室，4研究生学院，内蒙古自治区呼和浩特市   010110
  • 收稿日期:2022-10-24 接受日期:2023-03-16 出版日期:2024-04-28 发布日期:2023-08-22
  • 通讯作者: 王星，博士，副教授，内蒙古医科大学基础医学院，人体解剖学教研室，数字医学中心，内蒙古自治区呼和浩特市 010110 李志军，教授，博士生导师，内蒙古医科大学基础医学院，人体解剖学教研室，数字医学中心，内蒙古自治区呼和浩特市 010110
  • 作者简介:李琨，女，1988年生，汉族，内蒙古自治区呼和浩特市人，在读博士，主要从事脊柱与脊髓的数字化研究。 张少杰，男，1981年生，汉族，内蒙古自治区呼和浩特市人，博士，主要从事脊柱与脊髓的数字化研究。
  • 基金资助:
    内蒙古自治区自然科学基金项目(2022QN03002)，项目负责人：李琨；内蒙古医科大学青年基金项目(YKD2020QNCX055)，项目负责人：李琨；内蒙古医科大学英才培育项目(YCPY2022019)，项目负责人：李琨；内蒙古医科大学大学生创新创业项目(202110132025，202210132031，202210132011)，项目负责人：李琨；国家自然科学基金资助项目(81860383)，项目负责人：李志军；内蒙古自治区自然科学基金资助项目(2020LH08021)，项目负责人：李志军；国家自然科学基金资助项目(81860382)，项目负责人：王星；内蒙古自治区自然科学基金资助项目(2020MS03061)，项目负责人：王星；内蒙古自治区高等学校青年科技英才支持计划资助项目(NJYT22009)，项目负责人：王星；内蒙古自治区科技计划项目(2019GG158)，项目负责人：王星；内蒙古医科大学科研重点项目(YKD2021ZD011)，项目负责人：王星；内蒙古自治区卫生健康委医疗卫生科技计划项目(202201217)，项目负责人：王星；内蒙古自治区自然科学基金资助项目(2019MS08017)，项目负责人：张少杰

Micromorphological characteristics of the pedicle of the lower cervical vertebra

Li Kun1, 2, Zhang Shaojie1, 2, Shi Jun3 , Wang Jian1 , Liu Yanan4 , Duo Lan4 , Yang Yang4 , Hao Yunteng4 , Li Zhijun1, 2, Wang Xing1, 2   

  1. 1Department of Anatomy, 2Digital Medicine Center, 3Department of Physiology, 4Graduate School, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China
  • Received:2022-10-24 Accepted:2023-03-16 Online:2024-04-28 Published:2023-08-22
  • Contact: Wang Xing, PhD, Associate professor, Department of Anatomy, and Digital Medicine Center, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China Li Zhijun, Professor, Doctoral supervisor, Department of Anatomy, and Digital Medicine Center, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China
  • About author:Li Kun, Doctoral candidate, Department of Anatomy, and Digital Medicine Center, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China Zhang Shaojie, PhD, Department of Anatomy, and Digital Medicine Center, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China
  • Supported by:
    Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2022QN03002 (to LK); Youth Fund Project of Inner Mongolia Medical University, No. YKD2020QNCX055 (to LK); Talent Cultivation Project of Inner Mongolia Medical University, No. YCPY2022019 (to LK); College Student Innovation and Entrepreneurship Project of Inner Mongolia Medical University, No. 202110132025, 202210132031, 202210132011 (to LK); National Natural Science Foundation of China, No. 81860383 (to LZJ); Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2020LH08021 (to LZJ); National Natural Science Foundation of China, No. 81860382 (to WX); Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2020MS03061 (to WX); Youth Science and Technology Talent Support Program of Higher Education Institutions in Inner Mongolia Autonomous Region, No. NJYT22009 (to WX); Science and Technology Plan Project of Inner Mongolia Autonomous Region, No. 2019GG158 (to WX); Key Research Project of Inner Mongolia Medical University, No. YKD2021ZD011 (to WX); Health Commission Medical and Health Technology Plan Project of Inner Mongolia Autonomous Region, No. 202201217 (to WX); Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2019MS08017 (to ZSJ)

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摘要：


文题释义：

椎弓根：是椎弓的一部分，起于椎体后上部，短而细。椎弓根是椎间孔的组成部分。
Micro-CT：采用了与普通临床CT不同的微焦点X射线球管，分辨率高达几个微米，仅次于同步加速X射线成像设备的水平，具有良好的“显微”作用。


背景：下颈椎椎弓根是脊柱后柱的主要应力部位，对人体重心稳定的维持及减轻振荡有重要意义，目前对其内部骨小梁特征、椎弓根与关节突及椎体的连接部位的特征研究鲜有报道，迫切需要了解椎弓根的精细解剖结构及其各部分关系和功能。

目的：通过Micro-CT扫描下颈椎椎骨标本以观测椎弓根显微解剖学形态，测量和分析正常下颈椎椎弓根内骨小梁的微结构和形态计量学参数以评估颈椎的安全性能。
方法：选取31套干燥成人下颈椎骨骼标本C3-C7进行Micro-CT扫描，通过对椎弓根内骨小梁感兴趣区的勾选与重建，观察下颈椎椎弓根内骨小梁的形态特征及走形方向，检测骨微细结构参数，并分析比较C3-C7椎弓根骨微细结构的差异性。

结果与结论：①Micro-CT图像显示下颈椎椎弓根的蜂窝样骨小梁呈复杂的网状微结构，靠近皮质骨的骨小梁呈板层状，结构相对致密，并向前方椎体部和向后方关节突椎板部延伸；板状骨小梁向髓腔伸展转化为网状结构，再转化为杆状骨小梁，髓腔内杆状骨小梁分布稀疏；②骨小梁各形态参数统计结果显示，C4、C5椎弓根的骨小梁骨体积分数高于C7(P < 0.05)，C7椎弓根的骨表面积/骨体积高于C3、C4、C6(P < 0.05)，C7椎弓根的骨小梁骨表面密度高于C3、C4、C5、C6(P < 0.05)，C7椎弓根的骨小梁厚度高于C3、C4、C5(P < 0.05)，左侧椎弓根骨小梁的骨表面积/骨体积、骨表面密度比较大于右侧(P < 0.05)；③该文总结了C3-C7微结构变化规律，其中C7椎弓根的负荷能力及应力较差，该区发生损伤的风险较高。

https://orcid.org/0000-0002-8329-3368(李琨) 

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

关键词: 脊柱, 下颈椎, 椎弓根, Micro-CT, 显微形态, 骨小梁

Abstract: BACKGROUND: The lower cervical vertebral pedicle is the main stress site of the posterior column of the spine, which is of great significance for the maintenance of the stability of the human center of gravity and the reduction of shock. At present, there are few reports on the characteristics of the internal bone trabeculae, and the characteristics of the joint site of the vertebral pedicle with the articular process and the vertebral body. It is urgent to understand the fine anatomical structure of the vertebral pedicle and the relationship and function of each part.
OBJECTIVE: To observe the microanatomical morphology of the vertebral pedicle by Micro-CT scanning of cervical vertebra specimens, and to measure and analyze the microstructure and morphometric parameters of the bone trabecula in the cervical pedicle under normal conditions to evaluate the safety performance of the cervical spine.
METHODS: Micro-CT scanning was performed on 31 sets of cervical vertebrae C3-C7. By checking and reconstructing the areas of interest in the bone trabecular within the vertebral pedicle, the morphological characteristics and distribution direction of the bone trabecular within the cervical pedicle were observed, and the bone microstructure parameters were detected, and the differences in the bone microstructure of the C3-C7 vertebral pedicle were analyzed and compared.
RESULTS AND CONCLUSION: (1) The Micro-CT images showed that the honeycomb bone trabeculae of the pedicle of the lower cervical spine presented a complex network of microstructures. The trabeculae near the cortical bone were lamellar and relatively compact, extending forward toward the vertebral body and backward toward the articular process lamina. Abatoid bone trabeculae extended into the medullary cavity and transformed into a network structure, and then into rod-shaped bone trabeculae. The rod-shaped bone trabeculae were sparsely distributed in the medullary cavity. (2) Statistical results of morphological parameters of bone trabeculae showed that bone volume fraction values in C4 and C5 were higher than that in C7 (P < 0.05). The bone surface/bone volume value in C7 was higher than that in C3, C4 and C6 (P < 0.05). The bone surface density of bone trabeculae in C7 was higher than that in C3, C4, C5 and C6 (P < 0.05). Trabecular thickness in C7 was higher than that in C3, C4 and C5 (P < 0.05). Bone surface/bone volume and bone surface density of the left pedicle bone trabecular were greater than those on the right side (P < 0.05). (3) The microstructural changes of C3-C7 were summarized, in which the load capacity and stress of the C7 pedicle were poor, and the risk of injury was high in this area.

Key words: spinal column, lower cervical vertebra, pedicle, Micro-CT, microscopic morphology, bone trabeculae

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