中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (35): 5649-5652.doi: 10.12307/2023.856

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

大鼠小梁网组织表观弹性模量与组织弹性模量关系的数值模拟

王  川1，王金航1，刘润東1，张  静2，李  林3，刘志成4   

  1. 1首都医科大学，燕京医学院，医学影像学学系，北京市  101300；2徐州医科大学，医学影像学院，江苏省徐州市  221004；首都医科大学，生物医学工程学院，3生物医学信息学系，4生物力学与康复工程系，北京市  100069
  • 收稿日期:2022-09-28 接受日期:2022-12-09 出版日期:2023-12-18 发布日期:2023-06-02
  • 通讯作者: 刘志成，教授，首都医科大学，生物医学工程学院，生物力学与康复工程系，北京市 100069
  • 作者简介:王川，男，1985年生，北京市人，汉族，工学博士，讲师，主要从事眼生物力学研究。

Numerical simulation of the relationship between apparent elastic modulus and tissue elastic modulus of the trabecular meshwork in rats

Wang Chuan1, Wang Jinhang1, Liu Rundong1, Zhang Jing2, Li Lin3, Liu Zhicheng4   

  1. 1Department of Medical Imaging, Yanjing Medical College, Capital Medical University, Beijing 101300, China; 2School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China; 3Department of Biomedical Informatics, 4Department of Biomechanics and Rehabilitation Engineering, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
  • Received:2022-09-28 Accepted:2022-12-09 Online:2023-12-18 Published:2023-06-02
  • Contact: Liu Zhicheng, Professor, Department of Biomechanics and Rehabilitation Engineering, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
  • About author:Wang Chuan, PhD, Lecturer, Department of Medical Imaging, Yanjing Medical College, Capital Medical University, Beijing 101300, China

PDF

120

摘要：


文题释义：

小梁网：巩膜静脉窦与前房角之间的小梁结构，内含胶原纤维、弹性纤维，并被内皮细胞覆盖，是房水循环主要通道。
表观弹性模量：通过组织局部原子力显微镜压痕实验获取的压入位置处的弹性模量值。
组织弹性模量：通过组织整体压缩实验获取整个组织的弹性模量值，称为组织弹性模量。

背景：小梁网的组织力学特性与房水流出阻力密切相关。考虑到小梁网组织的多孔结构特点，利用原子力显微镜压痕技术可获取组织的表观弹性模量，但该方法无法获取组织弹性模量信息，因此，小梁网组织表观弹性模量与组织弹性模量之间的关系缺少定量研究。
目的：通过有限元模拟进一步分析小梁网表观弹性模量和组织弹性模量的关系。
方法：基于大鼠小梁网组织的双光子共聚焦图像构建该组织的三维结构模型，利用有限元分析模拟压痕实验，获取不同位置处组织表观弹性模量，模拟整体压入实验获取组织的弹性模量。

结果与结论：实验获取了基于原子力显微镜压痕实验的一阶Ogden模型参数范围，模拟原子力显微镜压痕实验获取的小梁网组织表观弹性模量平均值介于当压缩比为1%-2%时组织的弹性模量范围之内。实验量化了小梁网组织表观弹性模量和弹性模量的关系，为该组织的力学特性的研究提供了方法学参考。

https://orcid.org/0000-0002-9037-4230(王川)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 小梁网组织, 弹性模量, 有限元分析, 压痕实验, Hertz模型

Abstract: BACKGROUND: The tissue mechanical properties of trabecular meshwork are closely related to the resistance of the aqueous humor outflow. Considering the porous structure of trabecular meshwork, the apparent elastic modulus of trabecular meshwork tissue can be obtained by atomic force microscope indentation technique. However, the information of tissue elastic modulus cannot be obtained by this method. Therefore, there is a lack of quantitative research on the relationship between apparent and tissue elastic modulus of the trabecular meshwork.
OBJECTIVE: To further analyze the relationship between apparent and tissue elastic modulus of the trabecular meshwork by finite element simulation.
METHODS: Based on the two-photon confocal images of rat trabecular meshwork, a three-dimensional structure model of the trabecular meshwork was constructed. The finite element analysis was used to simulate the indentation experiment to obtain the apparent elastic modulus of the trabecular meshwork at different positions, and the tissue elastic modulus was obtained by simulating the whole indentation experiment.
RESULTS AND CONCLUSION: The parameters of the first-order Ogden model based on the atomic force microscope indentation experiment were obtained. The average value of the apparent elastic modulus of the trabecular meshwork obtained by simulating the atomic force microscope indentation experiment was within the range of the tissue elastic modulus of the trabecular meshwork tissue when the compression ratio was 1%-2%. This study quantifies the relationship between apparent and tissue elastic modulus of trabecular meshwork tissue and provides a methodological reference for the study of mechanical properties of trabecular meshwork tissue.

Key words: trabecular meshwork, elastic modulus, finite element analysis, indentation test, Hertz model

中图分类号: