中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3556-3559.doi: 10.3969/j.issn.1673-8225.2012.19.028

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

人参皂甙Rg3在体外对白血病K562细胞增殖和诱导分化的影响**★

肖  凤1，2，刘  彬1，张建平2，王伴青2，方木水2，胡  玮2，孙续禄2，朱清仙1   

  1. 1南昌大学医学院，江西省南昌市  330006；2井冈山大学医学院，江西省吉安市 343000
  • 收稿日期:2011-12-11 修回日期:2012-03-05 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: 朱清仙，教授，南昌大学医学院，江西省南昌市 330006
  • 作者简介:肖凤★，女，1965年生，江西省吉安市人，汉族，2005年南昌大学毕业，硕士，教授，主要从事肿瘤发生机制及肿瘤复发与转移的研究。xiaofeng1008@yahoo.com.cn
  • 基金资助:

    江西省自然基金资助项目(2009GZY0123)；江西省教育厅科技课题(GJJ09337)。

Effects of ginsenoside Rg3 on proliferation and induced differentiation of erythroleukemia cell line K562 in vitro 

Xiao Feng1, 2, Liu Bin1, Zhang Jian-ping2, Wang Ban-qing2, Fang Mu-shui2, Hu Wei2, Sun Xu-Lu2, Zhu Qing-Xian1   

  1. 1Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2Medical College of Jinggangshan University, Jian 343000, Jiangxi  Province, China
  • Received:2011-12-11 Revised:2012-03-05 Online:2012-05-06 Published:2012-05-06
  • Contact: Zhu Qing-xian, Professor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Xiao Feng★, Master, Professor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China xiaofeng1008@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Jiangxi Province, No. 2009GZY0123*; Science and Technology Topic of Education Department of Jiangxi Province, No. GJJ09337*

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摘要：

背景：文献报道人参皂甙Rg3具有抗肿瘤作用，但对白血病作用研究很少。
目的：观察人参皂甙Rg3对白血病K562细胞的作用，并探讨其相关机制。
方法：以白血病K562细胞为靶细胞，实验分为对照组和人参皂甙Rg3组，人参皂甙Rg3组分别添加10，20，40，80，       100 mg/L Rg3。
结果与结论：MTT检测结果显示，不同浓度人参皂甙Rg3组K562细胞生长抑制率均显著高于对照组(P < 0.05~0.01)。NBT结果显示，人参皂甙Rg3组培养1，2，3 d K562细胞还原率均高于对照组(P < 0.01)；人参皂甙Rg3组部分K562细胞体积变小，核仁消失，同时阳性细胞增多，细胞向成熟分化；流式细胞仪检测显示人参皂甙Rg3组培养2 d细胞周期G2期增高了3.84倍。提示人参皂甙Rg3在体外可抑制K562细胞增殖活性，其机制可能与人参皂甙Rg3将K562细胞周期阻滞在G2期，使细胞不能进行正常的有丝分裂，从而抑制细胞增殖有关。
 

关键词: 人参皂甙Rg3, K562细胞, 硝基蓝四氮唑蓝, 增殖, 诱导分化

Abstract:

BACKGROUND: Evidence exists that ginsenoside Rg3 can inhibit the growth of carcinoma cells. However, there are fewer studies describing ginsenoside Rg3 effects on leukemia.
OBJECTIVE: To investigate the effects of ginsenoside Rg3 on proliferation of erythroleukemia cell line K562 and the underlying mechanism.
METHODS: Erythroleukemia cell line K562 was used as target cell. The cells were divided into a control group and an Rg3 group. 10, 20, 40, 80, 100 mg/L Rg3 was added in the Rg3 group.
RESULTS AND CONCLUSION: The MTT colorimetric assay indicated that K562 growth inhibition rate was significantly higher in the Rg3 groups than in the control group (P < 0.05-0.01). The nitroblue tetrazolium assay showed that after culture for 1, 2, 3 days, the reducing power of K562 cells in the Rg3 group was significantly higher than in the control group (P < 0.01). In the Rg3 group, some K563 cell somas became small, nucleoli disappeared, nitroblue tetrazolium positive cells increased, and cells developed toward maturation. Flow cytometry detection showed that after 2 days of culture, the cell cycle at G2 phase in Rg3 group was 3.84 times higher than that in the control group. Ginsenoside Rg3 can inhibit the proliferation of erythroleukemia cell line K562 in vitro. The underlying mechanism may be related to a fact that ginsenoside Rg3 blocks K562 cell cycle at G2 stage, hinders normal mitosis and thereby inhibits cell proliferation.

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