中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7843-7848.doi: 10.3969/j.issn.2095-4344.2012.42.010

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

自拟通痹汤对Ⅱ型胶原性关节炎关节软骨的作用

杨军平，邱丽瑛，肖 亮，王 莹   

  1. 江西中医学院附属医院，江西省南昌市 330006
  • 收稿日期:2012-02-14 修回日期:2012-04-06 出版日期:2012-10-14 发布日期:2012-10-14
  • 作者简介:杨军平★，男，1973年生，江西省瑞金市人，汉族，2004年江西中医学院毕业，硕士，讲师，副主任技师，主要从事细胞免疫方面的研究。 yyfyjp0507@126.com

Effect of self-prescribed Tongbi decoction on the articular cartilage following typeⅡcollagen arthritis

Yang Jun-ping, Qiu Li-ying, Xiao Liang, Wang Ying   

  1. Affiliated Hospital of Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, Jiangxi Province, China
  • Received:2012-02-14 Revised:2012-04-06 Online:2012-10-14 Published:2012-10-14
  • About author:Yang Jun-ping★ Master, Lecturer, Associate chief technician, Affiliated Hospital of Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, Jiangxi Province, China yyfyjp0507@126.com

PDF

464

被引次数

9

摘要：

背景：自拟通痹汤是喻建平教授临床观察多年的临床经验方，适用于类风湿关节炎湿热痹阻证。
目的：验证自拟通痹汤对Ⅱ型胶原性关节炎大鼠的足肿胀的抑制作用，以及对自身抗体IgG、T细胞亚群、细胞因子白细胞介素1β和肿瘤坏死因子α的调节作用及对踝关节局部病理损伤的治疗作用。
方法： Wistar大鼠分为6组。除正常组外，以Ⅱ型胶原建立大鼠Ⅱ型胶原性关节炎动物模型。模型组：给予给药大剂量组等体积的蒸馏水；对照组：给予泼尼松5.0 mg/kg；自拟通痹汤大、中、小剂量组，剂量分别为11.6，5.8和2.9 g/kg。正常对照组大鼠右后足趾皮下注射生理盐水0.1 mL。1周后以相同剂量于每只大鼠尾部加强注射1次。造模2周后开始给药，均为灌胃给药，1次/d，连续2周。
结果与结论：结果表明，自拟通痹汤对Ⅱ型胶原诱导的大鼠关节炎关节肿胀有明显的抑制作用；对大鼠血清IgG水平升高有明显的抑制作用；对大鼠胸腺CD4+和CD8+ T淋巴细胞的异常有明显的免疫调节作用；对大鼠血清肿瘤坏死因子α有明显的抑制作用。自拟通痹汤各剂量组大鼠滑膜水肿、炎性细胞浸润、滑膜增生、血管翳生成、软骨破坏等病理损害均得到改善。

关键词: 类风湿性关节炎, 自拟通痹汤, 佐剂性关节炎, Ⅱ型胶原, 胶原性关节炎, 免疫调节

Abstract:

BACKGROUND: The self-prescribed Tongbi decoction is an empirical formula that has been observed in clinic for many years by Prof. Yu Jian-ping, and it is applied to treat dampness-heat blockage syndrome of rheumatoid arthritis.
OBJECTIVE: To verify the inhibitory effect of self-prescribed Tongbi decoction on the hind paw swelling in adjuvant-induced arthritis (AIA) rats, and its regulative effect on autoantibody IgG, T cell subgroups, interleukin 1β and tumor necrosis factor α, as well as its therapeutic role on ankle pathological injuries.
METHODS: Wistar rats were divided into six groups. An AIA rat model with collagen Ⅱ was established in all groups except for the normal control group. The rats of the model group were injected an equal volume of distilled water as the high-dose administration group; rats of the control group were given 5.0 mg/kg prednisone; rats in the high-dose, moderate-dose and low-dose administration groups were treated with 11.6, 5.8 and 2.9 g/kg self-prescribed Tongbi decoction, respectively. The rats in the normal control group were injected 0.1 mL normal saline into the rear toe of rats. After 1 week, all rats received a booster injection with the same dose on the tails. After modeled for 2 weeks, the rats were given intragastric administration, once a day for 2 weeks.
RESULTS AND CONCLUSION: The self-prescribed Tongbi decoction has a significant inhibitory effect on the hind paw swelling in collagen-induced arthritis rats, and a significant inhibitory effect on the increase of IgG serum concentration, a significant immunomodulatory effect on the abnormal CD4, CD8 and T lymphocytes as well as a significant inhibitory effect on tumor necrosis factor α. The synovial membrane edema, inflammatory cell infiltration, synovial proliferation, pannus generating and cartilage destruction achieved an improvement in different doses of self-prescribed Tongbi decoction groups.

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