中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (49): 7314-7319.doi: 10.3969/j.issn.2095-4344.2016.49.003

• 脑及脊髓损伤动物模型 Animal models of brain and spinal cord injuries • 上一篇    下一篇

针灸对脑性瘫痪幼鼠模型行为学的影响及其机制

娄元俊1，刘  阳2，单海军1，曹彩红1，介小素1   

  1. 1河南中医学院第二附属医院儿童康复科，河南省郑州市  450000；2河南中医学院研究生处，河南省郑州市   450000
  • 收稿日期:2016-09-02 出版日期:2016-11-30 发布日期:2016-11-30
  • 通讯作者: 刘阳，硕士，讲师，河南中医学院研究生处，河南省郑州市 450000
  • 作者简介:娄元俊，男，1978年生，河南省新乡市人，汉族，硕士，主治医师，主要从事小儿神经康复方面的研究。
  • 基金资助:

    河南中医学院苗圃工程项目(MP2011-16)

Effect of acupuncture of “Xingnao Kaiqiao” on the behavior of an immature rat model of cerebral palsy and the underlying mechanism

Lou Yuan-jun1, Liu Yang2, Shan Hai-jun1, Cao Cai-hong1, Jie Xiao-su1   

  1. 1Department of Children Rehabilitation, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China; 2School of Graduate of Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China
  • Received:2016-09-02 Online:2016-11-30 Published:2016-11-30
  • Contact: Liu Yang, Master, Lecturer, School of Graduate of Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China
  • About author:Lou Yuan-jun, Master, Attending physician, Department of Children Rehabilitation, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China
  • Supported by:

    the Nursery Engineer of Henan University of Chinese Medicine, No. MP2011-16

PDF

517

被引次数

21

摘要：

文章快速阅读：


文题释义：
醒脑开窍针刺法：是天津中医学院第一附属医院石学敏教授所创立的一种治疗中风病的针法，它以传统的中医理论及各家学说为基础，结合现代医学的有关理论，认为中风的基本病理改变为“神窍匿闭”，据此一观点，制定了以“醒脑开窍为主，疏通经络，滋补肝肾为辅”的治疗原则，在此原则指导下治疗中风病的针法，简称“醒脑开窍针刺法”。
动物行为学：是研究动物个体和动物社群为适应内外环境变化(刺激)所作出反应的学科。由于行为学研究的是动物的行为，因此，它也被称为“动物行为学”。研究方法为应用Mortis水迷宫检测幼鼠空间学习记忆能力等。
摘要
背景：醒脑开窍针刺法可以提高脑血流量，降低炎性反应，促进脑组织神经修复，这些作用都能够与脑性瘫痪(简称脑瘫)的治疗方向一致。
目的：探讨醒脑开窍针刺法对脑瘫幼鼠行为学的影响和其作用机制。
方法：①建立脑瘫幼鼠模型，将其随机分为正常对照组、模型对照组、醒脑开窍针刺组；②醒脑开窍针刺法主穴取内关、人中、三阴交，副穴取极泉、尺泽、委中穴进行针刺，模型组和正常对照组不做任何处理；③治疗24 d后，测量体质量，记录撕除前爪腹侧面胶布的时间，应用Mortis水迷宫检测幼鼠空间学习记忆能力，酶联免疫吸附法检测血清白细胞介素6和肿瘤坏死因子因子α水平。
结果与结论：①体质量增加率：正常对照组>醒脑开窍针刺组>模型组，各组之间比较差异有显著性意义(P < 0.05)；②撕除前爪腹侧面胶布的时间：模型组>醒脑开窍针刺组>正常对照组，各组之间比较差异有显著性意义(P < 0.05)；③水迷宫实验结果：醒脑开窍针刺法能改善脑瘫幼鼠认知能力，减少寻找平台潜伏期和游泳距离，与模型组对比差异有显著性意义(P < 0.05)；④炎症因子水平：醒脑开窍针刺法组血清肿瘤坏死因子α和白细胞介素6水平均有所下降，与模型组对比差异有显著性意义(P < 0.05)；⑤由此可见，醒脑开窍针刺法可降低缺血缺氧脑瘫幼鼠模型血清肿瘤坏死因子α和白细胞介素6炎症细胞因子的含量，从而改善脑瘫症状，提高认知能力。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程
ORCID: 0000-0001-5791-0214(娄元俊)

关键词: 实验动物, 神经损伤与修复动物模型, 醒脑开窍针刺法, 脑瘫, 幼鼠, 行为学, 肿瘤坏死因子α, 白细胞介素6

Abstract:

BACKGROUND: Acupuncture of “Xingnao Kaiqiao” can treat cerebral palsy by increasing cerebral blood flow, decreasing inflammatory reaction and promoting cerebral nerve repair.
OBJECTIVE: To explore the influence of acupuncture of “Xingnao Kaiqiao” on behavior of immature rats with cerebral palsy and the underlying mechanism.
METHODS: (1) The immature rat model of cerebral palsy was established and then randomized into control, model and acupuncture groups. (2) The immature rats in the acupuncture group were treated with acupuncture at major points of Neiguan (P 6), Renzhong (Du 26) and Sanyinjiao (Sp 6), as well as auxiliary points of Jiquan (H 1), Chize (Lu 5) and Weizhong (UB 40), while the model and control groups received no intervention. (3) Twenty-four days later, the body mass and the time removing the mackintosh at forepassed center were measured and recorded, the spatial learning and memory ability was detected by Mortis water maze test, and the serum levels of tumor necrosis factor alpha and interleukin-6 were detected by ELISA.
RESULTS AND CONCLUSION: (1) The order of the body mass gained was as follows: control group > acupuncture group > model group (P < 0.05). (2) The time removing the mackintosh at forepassed center was the longest in the model group, followed by the acupuncture group, and shortest in the control group (P < 0.05). (3) Acupuncture method significantly improved the cognitive ability of immature rats, and reduced the latency of searching for platform and swimming distance compared with the model group (P < 0.05). (4) The serum levels of tumor necrosis factor alpha and interleukin-6 in the acupuncture group were significantly lower than those in the model group (P < 0.05). (5) In conclusion, the acupuncture of “Xingnao Kaiqiao” can improve the symptoms and cognitive ability of the immature rat model of cerebral palsy by up-regulating the serum levels of tumor necrosis factor alpha and interleukin-6.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Cerebral Palsy, Acupuncture, Tumor Necrosis Factor-alpha, Interleukin-6, Tissue Engineering

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