中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (43): 6438-6444.doi: 10.3969/j.issn.2095-4344.2016.43.008

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

具有微弧氧化-硅烷-褪黑素复合膜层钛合金种植体的体内骨结合

李德超，刘惠萍，李慕勤，刘  苗
  

  1. 佳木斯大学，黑龙江省高校生物医学材料重点实验室，黑龙江省佳木斯市  154007
  • 收稿日期:2016-07-23 出版日期:2016-10-21 发布日期:2016-10-21
  • 通讯作者: 李慕勤，教授，佳木斯大学，黑龙江省高校生物医学材料重点实验室，黑龙江省佳木斯市154007
  • 作者简介:李德超，男，1970年生，黑龙江省佳木斯市人，汉族，教授，博士，主要从事口腔种植方面的研究。
  • 基金资助:

    国家自然科学基金(31370979)；黑龙江省高校创新团队建设计划项目(2012TD010)；佳木斯大学协同创新中心(2011xtcx2016-02)

In vivo osseointegration of micro-arc oxidation-silane-melatonin-covered titanium alloy implant

Li De-chao, Liu Hui-ping, Li Mu-qin, Liu Miao
  

  1. Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
  • Received:2016-07-23 Online:2016-10-21 Published:2016-10-21
  • Contact: Li Mu-qin, Professor, Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
  • About author:Li De-chao, M.D., Professor, Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 31370979; the Innovation Team Building Project in Heilongjiang Provincial Colleges, No. 2012TD010; Collaborative Innovation Center of Jiamusi University, No. 2011xtcx2016-02

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被引次数

1

摘要：

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文题释义：
褪黑素：已被证实对骨质疏松症、牙科植入物的骨整合作用及牙本质的形成均有作用。它的作用为抗氧化、清除自由基、增加免疫和护骨，是刺激成骨和骨改建的重要调节者，抑制破骨细胞的分化，促进成骨细胞合成和分化；早期阶段(2-4周)褪黑素可能刺激更多的细胞分化，加速皮质骨合成、类骨质分化，导致新生皮质骨的长度、宽度均增加。
微弧氧化：又称微等离子体氧化，是通过电解液与相应电参数的组合，在铝、镁、钛及其合金表面依靠弧光放电产生的瞬时高温高压作用，生长出以基体金属氧化物为主的陶瓷膜层。

背景：钛合金种植体与骨界面结合的强度不理想，延长了种植体与周围骨组织的愈合时间。
目的：为进一步提高钛合金口腔种植体的早期愈合能力，对钛合金种植体进行表面改性，观察其骨结合能力。
方法：分别制备具有微弧氧化膜层的钛合金种植体(设为A组)、微弧氧化-硅烷复合膜层的钛合金种植体(设为B组)与微弧氧化-硅烷-褪黑素复合膜层的钛合金种植体(设为C组)。将3种种植体分别置入新西兰大白兔下颌骨中，置入后2，4，6周，采用锥形束CT、荧光显微镜、扭力实验检测种植体-骨界面早期成骨情况。
结果与结论：①锥形束CT检查结果：置入后6周，A组种植体与下颌骨结合不紧密；C组种植体与下颌骨结合紧密，骨结合最好；B组骨结合情况介于两者之间；②荧光显微镜观察结果：3组种植体置入4周的荧光最强，6周时稍有减弱，C组不同时间点的荧光强度高于A、B组；③扭力实验结果：随着置入时间的延长，3组种植体骨结合力逐渐增强，C组不同时间点的骨结合力高于A、B组(P < 0.05)；④结果表明：具有微弧氧化-硅烷-褪黑素复合膜层的钛合金种植体具有较高的骨结合能力活性，可促进早期成骨。

关键词: 生物材料, 口腔生物材料, 钛种植体, 种植体-骨界面, 偶联剂, 褪黑素, 骨结合, 国家自然科学基金

Abstract:

BACKGROUND: Imperfect strength of titanium alloy implant-bone interface delays bone healing.
OBJECTIVE: In order to further improve the early healing ability of titanium dental implants, to make the surface modification of titanium alloy implant and observe the osseointegration ability of the implant.
METHODS: Titanium alloy implants were coated with micro-arc oxidation (group A), micro-arc oxidation-silane (group B), and micro-arc oxidation-silane-melatonin (group C) coatings, respectively. Three kinds of implants were placed into the mandible of New Zealand white rabbits, and 2, 4, 6 weeks after implantation, implant-bone interface osseointegration was observed using cone-beam CT, fluorescence microscopy, and torsion test.
RESULTS AND CONCLUSION: (1) Cone-beam CT examination: 6 weeks after implantation, the osseointegration of the implant with the mandible achieved the best results in the group C, followed by group B and group A. (2) Fluorescence microscope observation: the implants in the three groups exhibited the strongest fluorescence at 4 weeks and slightly weakened at 6 weeks, and the fluorescence intensity was higher in the group C than the other two groups. (3) Torsion test: with implantation time prolonged, the implant-bone osseointegration activity gradually increased in the three groups, while it was still highest in the group C (P < 0.05). In summary, our results show that the titanium alloy implant coated with micro-arc oxidation-silane-melatonin composite coating has higher osseointegration activity and promotes bone formation.

Key words: Dental Implants, Synostosis, Tissue Engineering

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