中国组织工程研究

• 干细胞学术探讨 stem cell academic discussion • 上一篇    

诱导多功能干细胞治疗脊髓损伤的现状及思考

刘文骏,张  辉,尹宗生   

  1. 安徽医科大学第一附属医院骨二科,安徽省合肥市  230001
  • 收稿日期:2013-04-09 修回日期:2013-05-27 出版日期:2013-07-02 发布日期:2013-07-02
  • 通讯作者: 尹宗生,博士生导师,主任医师,安徽医科大学第一附属医院骨二科,安徽省合肥市 230001 yinzongsheng@sina.com.cn
  • 作者简介:刘文骏★,男,1990年生,安徽省合肥市人,汉族,安徽医科大学在读硕士,主要从事脊髓损伤的干细胞治疗基础及临床研究。 Peter-green@163.com
  • 基金资助:

    国家自然科学基金项目(30973026);安徽省自然科学基金项目(1104060625)。

Thinking and actuality of induced pluripotent stem cells to treat spinal cord injury

Liu Wen-jun, Zhang Hui, Yin Zong-sheng   

  1. Second Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Hefei  23001, Anhui Province, China
  • Received:2013-04-09 Revised:2013-05-27 Online:2013-07-02 Published:2013-07-02
  • Contact: Yin Zong-sheng, Doctoral supervisor, Chief physician, Second Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Hefei 23001, Anhui Province, China yinzongsheng@sina.com.cn
  • About author:Liu Wen-jun★, Studying for master’s degree, Second Department of Orthopedics, the First Affiliated Hospital of Anhui Medical University, Hefei 23001, Anhui Province, China Peter-green@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973026*; the Natural Science Foundation of Anhui Province, No. 1104060625*

摘要:

背景:目前对脊髓损伤的治疗方法有激素冲击治疗,急诊手术减压及最新发展的干细胞治疗等方法。已有大量工作聚焦于干细胞治疗脊髓损伤的研究方面,并已成为热点。
目的:综述诱导多功能干细胞治疗脊髓损伤的相关研究现状及未来发展趋势。
方法:应用计算机检索PubMed 数据库1980年1月至2012年12 月期间的相关文章,检索关键词为“spine injury,induced pluripotent stem cells,IPS”。
结果与结论:共纳入35篇文章,均就诱导多能干细胞治疗脊髓损伤持赞同或支持态度。结果表明,诱导多功能干细胞直接来源于自体分离得到,解决了干细胞移植中伦理以及道德等问题,同时避免了异体排斥反应也为移植提供了大量的细胞来源。对于脊髓损伤的治疗方面目前只有动物实验及少量临床实验支持,但其在动物实验中效果良好,安全性较高。诱导多功能干细胞治疗脊髓损伤现存的问题主要是诱导多功能干细胞的诱导分化方法不成熟,并发症也较多。因此,诱导多功能干细胞的诱导分化及使用安全性将成为研究关键。

关键词: 干细胞, 干细胞学术探讨, 骨髓干细胞, 脊髓损伤, 诱导多功能干细胞, 并发症, 脊髓, 移植, 国家自然科学基金

Abstract:

BACKGROUND: Currently, therapies for spinal cord injury include steroid pulse therapy, surgical decompression and stem cell therapy. A lot of work has been focused on stem cell therapy for spinal cord injury which has become a hot spot.
OBJECTIVE: To summarize the research status and progress in induced pluripotent stem cells for treatment of spinal cord injury.
METHODS: A computer-based online search of PubMed (1980-01/2011-11) was performed for articles in English using the keywords of “spine injury, induced pluripotent stem cells, IPS”
RESULTS AND CONCLUSION: A total of 35 articles were included in result analysis, in which, stem cell therapy for treatment of spinal cord injury is agreed or supported. Induced pluripotent stem cells are isolated directly from the body, which solves ethical and moral issues in the transplantation of stem cells, meanwhile avoids allograft rejection and also provides a large source of cells. Stem cell therapy for spinal cord injury is widely used in animal experiments but few in clinical application. However, stem cell therapy has a good effect in the animal experiments, and shows a higher safety. The existing problems of induced pluripotent stem cells to treat spinal cord injury mainly include immature differentiating method of induced pluripotent stem cells and more complications. Therefore, induced differentiation and security of induced pluripotent stem cells for treatment of spinal cord injury will become a research key.

Key words: stem cells, stem cell academic discussion, bone marrow-derived stem cells, spinal cord injury, induced pluripotent stem cells, complications, spinal cord, transplantation, National Natural Science Foundation of China

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