中国组织工程研究

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

氧等离子处理人工晶状体材料的生物相容性

夏瑞静,徐  栩,罗  丽, 李玲琍,陈  浩   

  1. 温州医学院眼视光学院,浙江省温州市  325027
  • 收稿日期:2012-09-10 修回日期:2012-11-13 出版日期:2013-05-21 发布日期:2013-05-21
  • 通讯作者: 李玲琍,助理研究员,博士,温州医学院眼视光学院,浙江省温州市 325027 lll09182@126.com 陈浩,博士,副教授,硕士生导师,温州医学院眼视光学院,浙江省温州市 325027 chenhao823@mail.eye.ac.cn
  • 作者简介:夏瑞静,女,1989年生,浙江省温州市人,汉族,温州医学院本科在读,主要从事眼科生物材料的研究。 xiaruijing@126.com
  • 基金资助:

    浙江省重点科技创新团队项目(2011R09039-07),温州市科技局科技项目(Y20090245)和2010年浙江省大学生科技创新活动计划(新苗人才计划)项目(2010R413009)资助

Biocompatibility of intraocular lens via oxygen plasma treatment

Xia Rui-jing, Xu Xu, Luo Li, Li Ling-li, Chen Hao   

  1. Institute of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou  325027, Zhejiang Province, China
  • Received:2012-09-10 Revised:2012-11-13 Online:2013-05-21 Published:2013-05-21
  • Contact: Li Ling-li, M.D., Assistant researcher, Institute of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China Chen Hao, M.D., Associate professor, Master’s supervisor, Institute of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China chenhao823@mail.eye.ac.cn
  • About author:Xia Rui-jing, Institute of Ophthalmology & Optometry, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China xiaruijing@126.com
  • Supported by:

    Key Science and Technology Innovative Research Team of Zhejiang Province, No. 2011R09039-07; Wenzhou Municipal Science and Technology Bureau, No. Y20090245; Innovation Training Plan of Zhejiang Province for University Students (Xinmiao Talent Program 2011), No. 2010R413009

摘要:

背景:氧等离子处理可有效改善双组分室温硫化硅橡胶人工晶状体材料表面的亲水性能和生物相容性,但等离子体聚合形成的薄膜可能产生卷曲和破裂或因与基质是非共价键结合而产生剥离,影响材料本身的理化性能和光学性能。
目的:初步评价优选氧等离子处理表面改性后双组分室温硫化硅橡胶人工晶状体材料的体外生物相容性。
方法:采用氧等离子体表面改性技术修饰疏水性双组分室温硫化硅橡胶人工晶状体材料的表面,处理功率及时间分别为:20 W、50 W、100 W和30 s、1 min、3 min、10 min。应用视频光学接触角测定仪、傅里叶变换衰减全反射红外光谱和X射线光电子能谱分析材料表面亲水性和表面化学元素组成;扫描电子显微镜和原子力显微镜观察材料表面形貌。通过人晶状体上皮细胞黏附实验观察双组分室温硫化硅橡胶表面改性前后细胞的数量及形态改变。
结果与结论:最佳改性条件为100 W,30 s,20 mL/min,40 Pa。此条件改善了双组分室温硫化硅橡胶人工晶状体材料表面的亲水性,对材料表面无刻蚀作用,改善了人晶体上皮细胞在双组分室温硫化硅橡胶人工晶状体材料表面的生物相容性。

关键词: 生物材料, 材料生物相容性, 双组分室温硫化硅橡胶, 人工晶状体, 氧气等离子体, 表面改性, 生物相容性, 晶状体上皮细胞, 亲水性, 省级基金

Abstract:

BACKGROUND: Oxygen plasma treatment can greatly improve the hydrophilicity and biocompatibility of bicomponent room temperature vulcanized polydimethylsiloxane intraocular lens. However, plasma-aggravated thin film may curl, rupture or dissect due to binding with matrix noncovalent bond, thus affecting the physicochemical properties and optical performance of the material.
OBJECTIVE: To evaluate the in vitro biocompatibility of bicomponent room temperature vulcanized polydimethylsiloxane intraocular lens materials after oxygen plasma processing.
METHODS: Oxygen plasma treatment was employed to optimize the surface of intraocular lens materials using the surface modification technique. The power and duration were 20 W, 50 W, 100 W, and 30 seconds, 1 minute, 10 minutes, respectively. Water contact angle, fourier transform infrared spectroscopy-attenuated total reflection, X-ray photo electron spectroscopy analysis were adopted to analyze the hydrophilicity and the surface chemical composition of the material. Scanning electron microscopy and atomic force microscope were used to observe the surface morphology. Cellular adhesion was performed by seeding human lens epithelial cells onto the surface of the materials to evaluate the effect of surface modification on its biocompatibility.
RESULTS AND CONCLUSION: The surface modification of intraocular lens materials by oxygen plasma treatment under optimal conditions (100 W, 30 seconds, 20 mL/min, 40 Pa) could improve the surface hydrophilicity and biocompatibility of the material without etching effect.

Key words: biomaterials, material biocompatibility, bicomponent room temperature vulcanized polydimethylsiloxane, intraocular lens, oxygen plasma treatment, surface modification, biocompatibility, lens epithelial cells, hydrophilicity, provincial grants-supported paper

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