中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (16): 2472-2477.doi: 10.3969/j.issn.2095-4344.2017.16.00

• 骨组织构建 bone tissue construction • 上一篇    下一篇

大鼠筋膜源性细胞与肌源性细胞成骨能力的对比研究

宿海滨,李广恒   

  1. 郑州大学第一附属医院骨科,河南省郑州市  450000
  • 修回日期:2017-03-27 出版日期:2017-06-08 发布日期:2017-07-06
  • 通讯作者: 李广恒,博士,教授,博士生导师,郑州大学第一附属医院骨科,河南省郑州市 450000
  • 作者简介:宿海滨,男,1992年生,黑龙江省双鸭山市人,汉族,郑州大学在读硕士,主要从事骨科方面的研究。
  • 基金资助:

    国家自然科学基金资助项目(81472136)

Osteogenic ability of fascia- versus muscle-derived cells in rats

Su Hai-bin, Li Guang-heng   

  1. Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Revised:2017-03-27 Online:2017-06-08 Published:2017-07-06
  • Contact: Li Guang-heng, M.D., Professor, Doctoral supervisor, Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • About author:Su Hai-bin, Studying for master’s degree, Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81472136

摘要:

文章快速阅读:

 

文题释义:
碱性磷酸酶染色:
碱性磷酸酶在pH值9.4的环境下,以镁离子作为激活剂,能够把β-甘油酸磷脂水解出磷酸,磷酸与高浓度的钙盐结合形成无色的磷酸钙,再与硝酸钴作用形成磷酸钴,经硫化胺处理形成黑色的硫化钴沉淀于酶活性处。
细胞转染:是指将外源分子如DNA,RNA等导入真核细胞的技术。随着分子生物学和细胞生物学研究的不断发展,转染已经成为研究和控制真核细胞基因功能的常规工具。在研究基因功能、调控基因表达、突变分析和蛋白质生产等生物学试验中,其应用越来越广泛。

 

摘要
背景:
自体骨髓来源的间充质干细胞具有多分化潜能,能向成骨、成软骨细胞分化,加速骨组织及软骨组织的形成,但是成骨能力各不相同,哪种细胞的成骨能力更强有待进一步研究。
目的:比较大鼠筋膜源性细胞与肌源性细胞的成骨能力。
方法:从20只大鼠骨骼肌筋膜分离出筋膜源性细胞,从骨骼肌肌腹中分离出肌源性细胞。用流式细胞仪分选筋膜源性细胞和肌源性细胞,培养细胞。利用retro-BMP4病毒转染FDC-LacZ细胞。经过2次转染,转染的筋膜源性细胞通过LacZ和碱性磷酸酶的染色而测定出转染效率。
结果与结论:肌源性细胞产生的软骨细胞、钙盐沉积均多于筋膜源性细胞。提示大鼠肌源性细胞的成骨能力强于筋膜源性细胞。

 

 

ORCID:0000-0003-4425-788X(宿海滨)

关键词: 组织构建, 骨组织工程, 筋膜源性细胞, 肌源性细胞, 成骨能力, 骨组织工程, 骨缺损, 骨再生, 细胞转染, 细胞表面标记物, 国家自然科学基金

Abstract:

BACKGROUND: Multipotent differentiation ability enables mesenchymal stem cells from autologous bone marrow to differentiate into osteoblasts and chondroblasts, thereby promoting the formation of bones and cartilage. However, the osteogenic ability differs from each other, and whose osteogenic ability is the best still needs to be studied further.
OBJECTIVE: To compare the osteogenic ability of fascia- and muscle-derived stem cells in rats.
METHODS: Fascia- and muscle-derived cells were isolated from 20 rats, followed by flow cytometry sorting, and were then cultured. FDC-LacZ cells were transfected with retro-BMP4 virus twice. Afterwards, the transfection efficiency of fascia-derived cells was detected through LacZ and alkaline phosphatase staining.
RESULTS AND CONCLUSION: Compared with fascia-derived cells, muscle-derived cells showed stronger chondrogenic ability and produced more calcium deposition. These findings indicate that the osteogenic ability of muscle-derived cells is superior to that of fascia-derived cells in rats.

 

 

Key words: Muscle Cells, Cell Differentiation, Tissue Engineering

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