中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (10): 1631-1640.doi: 10.12307/2022.215

• 生物材料综述 biomaterial review • 上一篇    

组织修复和再生领域浓缩生长因子的应用优势

周  颐,刘笑言,向柄彦   

  1. 遵义医科大学第三附属医院(遵义市第一人民医院)关节外科,贵州省遵义市   563000
  • 收稿日期:2021-04-23 修回日期:2021-04-24 接受日期:2021-05-21 出版日期:2022-04-08 发布日期:2021-10-27
  • 通讯作者: 向柄彦,博士,教授,主任医师,硕士生导师,遵义医科大学第三附属医院(遵义市第一人民医院)关节外科,贵州省遵义市 563000
  • 作者简介:周颐,男,1991年生,四川省成都市人,汉族,遵义医科大学在读硕士
  • 基金资助:
    贵州省卫生健康委科学技术基金项目(gzwjkj-2020-1-129),项目负责人:向柄彦

Application advantages of concentrated growth factors in the field of tissue repair and regeneration

Zhou Yi, Liu Xiaoyan, Xiang Bingyan   

  1. Department of Joint Surgery, Third Affiliated Hospital of Zunyi Medical University (Zunyi First People’s Hospital), Zunyi 563000, Guizhou Province, China
  • Received:2021-04-23 Revised:2021-04-24 Accepted:2021-05-21 Online:2022-04-08 Published:2021-10-27
  • Contact: Xiang Bingyan, MD, Professor, Chief physician, Master’s supervisor, Department of Joint Surgery, Third Affiliated Hospital of Zunyi Medical University (Zunyi First People’s Hospital), Zunyi 563000, Guizhou Province, China
  • About author:Zhou Yi, Master candidate, Department of Joint Surgery, Third Affiliated Hospital of Zunyi Medical University (Zunyi First People’s Hospital), Zunyi 563000, Guizhou Province, China
  • Supported by:
    the Science and Technology Fund Project of Guizhou Provincial Health Commission, No. gzwjkj-2020-1-129 (to XBY)

摘要: 文题释义:
自体血小板浓缩物:是一种从自身血液中提取的生物活性制剂,富含多种内源性生长因子、血小板、纤维蛋白和白细胞,制备简便,且不存在免疫排斥反应,目前被广泛应用于组织的修复和再生领域。
浓缩生长因子:是目前最新一代自体血小板浓缩物,其两大核心结构为天然的三维纤维蛋白支架和多种内源性生长因子。与前2代不同,浓缩生长因子制备采用特定的变速离心,形成了更具韧性的纤维蛋白网络,为细胞的黏附和增殖提供了三维空间。变速离心也能够缓释出更高浓度的生长因子,并将白细胞和血小板更为稳定地聚合在浓缩生长因子内。此外,浓缩生长因子具有良好的可塑性和兼容性,能被制备成提取液、冻干材料、浓缩生长因子膜和浓缩生长因子培养基等。浓缩生长因子与组织工程材料相结合的应用方式已经成为目前组织修复和再生领域的研究热点。

背景:浓缩生长因子作为最新一代自体血小板浓缩生物制剂,因其具有天然的纤维蛋白支架和丰富的内源性生长因子,近年来已成为组织修复和再生领域的研究热点。
目的:总结浓缩生长因子在组织修复和再生领域中的研究进展,探讨目前研究阶段存在的问题,以期为浓缩生长因子的后续研究提供新的参考思路。
方法:文章以“浓缩生长因子、干细胞、组织修复、组织再生”为中文检索词,以“Concentrated growth factor,CGF,Stem cells,Tissue repair,Tissue regeneration”为英文检索词,在PubMed、EMbase、Medline和中国知网数据库进行全面检索。收集归纳近10年来浓缩生长因子在组织修复和再生领域应用的相关研究证据,根据严格纳入排除标准筛选文献,最终纳入96篇。

结果与结论:①文章从浓缩生长因子制备和应用的具体方法,促进组织修复和再生的原理,体外对细胞增殖和分化的作用,以及体内对组织修复和重建4个方面详细总结了浓缩生长因子目前的研究进展,讨论了现阶段存在的问题和未来可能的研究发展方向。②浓缩生长因子是一个在组织修复和再生领域具有潜力的自体生物活性因子,制备方法简便,应用方式灵活多变,完全取自自体血液,无免疫排斥反应,生物相容性良好。③浓缩生长因子的核心结构包括天然的纤维蛋白支架和多种内源性生长因子,在体外能促进多种干细胞的增殖、迁移、分化,浓缩生长因子与干细胞的复合体是干细胞治疗领域一种新的策略。④浓缩生长因子在体内能促进骨缺损的修复,其与组织工程材料联合所构建的局部生长因子缓释系统是延长生长因子缓释时间的有效方法。⑤浓缩生长因子在促进软组织修复、抗皮肤光老化、减少术后并发症等领域呈现出良好的效果,其已成为目前自体生物活性制剂个性化治疗的优先选择之一。

https://orcid.org/0000-0001-6157-2828 (周颐) ;https://orcid.org/0000-0001-6130-0224 (向柄彦)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料口腔生物材料纳米材料缓释材料材料相容性;组织工程

关键词: 浓缩生长因子, 骨缺损, 软组织, 内源性生长因子, 纤维蛋白支架, 干细胞分化, 细胞增殖, 局部缓释系统

Abstract: BACKGROUND: As the latest generation of autologous platelet concentrate biologics, concentrated growth factor has become a research hotspot in the field of tissue repair and regeneration due to its natural fibrin scaffold and abundant endogenous growth factors.
OBJECTIVE: To summarize the research progress of concentrated growth factors in the field of tissue repair and regeneration, as well as discuss the existing problems in the current research stage, in order to provide new reference ideas for the follow-up research of concentrated growth factors.
METHODS: This article used “concentrated growth factor, CGF, stem cells, tissue repair, tissue regeneration” as search terms. A comprehensive search was conducted on PubMed, EMbase, Medline, and CNKI. The relevant research evidence of the application of concentrated growth factor in the field of tissue repair and regeneration in the past 10 years were collected and summarized. The literature was screened based on strict inclusion and exclusion criteria. Finally, 96 articles were included. 
RESULTS AND CONCLUSION: (1) This article summarized the research progress of concentrated growth factor from four aspects: the specific method of preparation and application of concentrated growth factor, the principle of concentrated growth factor promoting tissue repair and regeneration, the effect on cell proliferation and differentiation in vitro, and the repair and reconstruction of tissue in vivo, and discussed the current research the existing problems. (2) Concentrated growth factor is an autologous bioactive factor with potential in the field of tissue repair and regeneration. The preparation method is simple and the application method is flexible and changeable. It is completely taken from autologous blood, so that it has great biocompatibility and few immune rejection reactions. (3) The core structure of concentrated growth factor includes a natural fibrin scaffold and a variety of endogenous growth factors, which can promote the proliferation, migration and differentiation of a variety of stem cells in vitro. (4) Concentrated growth factors can promote the repair of bone defects in vivo, and the local growth factor slow-release system constructed by combining it with tissue engineering materials is an effective way to extend the slow-release time of growth factors. (5) Concentrated growth factors have shown great effects in promoting soft tissue repairing, anti-skin photoaging, and reducing postoperative complications. It has become one of the preferred options for personalized treatment of autologous bioactive preparations.

Key words: concentrated growth factors, bone defect, soft tissue, endogenous growth factor, fibrin scaffold, stem cell differentiation, cell proliferation, local slow release system

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