中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (10): 1631-1640.doi: 10.12307/2022.215
• 生物材料综述 biomaterial review • 上一篇
周 颐,刘笑言,向柄彦
收稿日期:
2021-04-23
修回日期:
2021-04-24
接受日期:
2021-05-21
出版日期:
2022-04-08
发布日期:
2021-10-27
通讯作者:
向柄彦,博士,教授,主任医师,硕士生导师,遵义医科大学第三附属医院(遵义市第一人民医院)关节外科,贵州省遵义市 563000
作者简介:
周颐,男,1991年生,四川省成都市人,汉族,遵义医科大学在读硕士
基金资助:
Zhou Yi, Liu Xiaoyan, Xiang Bingyan
Received:
2021-04-23
Revised:
2021-04-24
Accepted:
2021-05-21
Online:
2022-04-08
Published:
2021-10-27
Contact:
Xiang Bingyan, MD, Professor, Chief physician, Master’s supervisor, Department of Joint Surgery, Third Affiliated Hospital of Zunyi Medical University (Zunyi First People’s Hospital), Zunyi 563000, Guizhou Province, China
About author:
Zhou Yi, Master candidate, Department of Joint Surgery, Third Affiliated Hospital of Zunyi Medical University (Zunyi First People’s Hospital), Zunyi 563000, Guizhou Province, China
Supported by:
摘要: 文题释义:
自体血小板浓缩物:是一种从自身血液中提取的生物活性制剂,富含多种内源性生长因子、血小板、纤维蛋白和白细胞,制备简便,且不存在免疫排斥反应,目前被广泛应用于组织的修复和再生领域。
浓缩生长因子:是目前最新一代自体血小板浓缩物,其两大核心结构为天然的三维纤维蛋白支架和多种内源性生长因子。与前2代不同,浓缩生长因子制备采用特定的变速离心,形成了更具韧性的纤维蛋白网络,为细胞的黏附和增殖提供了三维空间。变速离心也能够缓释出更高浓度的生长因子,并将白细胞和血小板更为稳定地聚合在浓缩生长因子内。此外,浓缩生长因子具有良好的可塑性和兼容性,能被制备成提取液、冻干材料、浓缩生长因子膜和浓缩生长因子培养基等。浓缩生长因子与组织工程材料相结合的应用方式已经成为目前组织修复和再生领域的研究热点。
背景:浓缩生长因子作为最新一代自体血小板浓缩生物制剂,因其具有天然的纤维蛋白支架和丰富的内源性生长因子,近年来已成为组织修复和再生领域的研究热点。
目的:总结浓缩生长因子在组织修复和再生领域中的研究进展,探讨目前研究阶段存在的问题,以期为浓缩生长因子的后续研究提供新的参考思路。
方法:文章以“浓缩生长因子、干细胞、组织修复、组织再生”为中文检索词,以“Concentrated growth factor,CGF,Stem cells,Tissue repair,Tissue regeneration”为英文检索词,在PubMed、EMbase、Medline和中国知网数据库进行全面检索。收集归纳近10年来浓缩生长因子在组织修复和再生领域应用的相关研究证据,根据严格纳入排除标准筛选文献,最终纳入96篇。
结果与结论:①文章从浓缩生长因子制备和应用的具体方法,促进组织修复和再生的原理,体外对细胞增殖和分化的作用,以及体内对组织修复和重建4个方面详细总结了浓缩生长因子目前的研究进展,讨论了现阶段存在的问题和未来可能的研究发展方向。②浓缩生长因子是一个在组织修复和再生领域具有潜力的自体生物活性因子,制备方法简便,应用方式灵活多变,完全取自自体血液,无免疫排斥反应,生物相容性良好。③浓缩生长因子的核心结构包括天然的纤维蛋白支架和多种内源性生长因子,在体外能促进多种干细胞的增殖、迁移、分化,浓缩生长因子与干细胞的复合体是干细胞治疗领域一种新的策略。④浓缩生长因子在体内能促进骨缺损的修复,其与组织工程材料联合所构建的局部生长因子缓释系统是延长生长因子缓释时间的有效方法。⑤浓缩生长因子在促进软组织修复、抗皮肤光老化、减少术后并发症等领域呈现出良好的效果,其已成为目前自体生物活性制剂个性化治疗的优先选择之一。
https://orcid.org/0000-0001-6157-2828 (周颐) ;https://orcid.org/0000-0001-6130-0224 (向柄彦)
中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程
中图分类号:
周 颐, 刘笑言, 向柄彦. 组织修复和再生领域浓缩生长因子的应用优势[J]. 中国组织工程研究, 2022, 26(10): 1631-1640.
Zhou Yi, Liu Xiaoyan, Xiang Bingyan. Application advantages of concentrated growth factors in the field of tissue repair and regeneration[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(10): 1631-1640.
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1.1.7 检索策略 见图1。
1.1.8 检索文献量 共检索出文献共3 252篇,筛选出2011-2021年的相关文献511篇。
浓缩生长因子:是目前最新一代自体血小板浓缩物,其两大核心结构为天然的三维纤维蛋白支架和多种内源性生长因子。与前2代不同,浓缩生长因子制备采用特定的变速离心,形成了更具韧性的纤维蛋白网络,为细胞的黏附和增殖提供了三维空间。变速离心也能够缓释出更高浓度的生长因子,并将白细胞和血小板更为稳定地聚合在浓缩生长因子内。此外,浓缩生长因子具有良好的可塑性和兼容性,能被制备成提取液、冻干材料、浓缩生长因子膜和浓缩生长因子培养基等。浓缩生长因子与组织工程材料相结合的应用方式已经成为目前组织修复和再生领域的研究热点。
文章从浓缩生长因子的制备应用,生物学原理,体外实验和体内研究四个方面详细总结了浓缩生长因子在组织修复和再生领域的研究进展,并提出浓缩生长因子联合组织工程材料所构建的局部生长因子缓释系统可能是实现生长因子持续缓释的有效方法之一。
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