中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (38): 5678-5683.doi: 10.3969/j.issn.2095-4344.2016.38.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

微米中药功能衬材：活血及促进骨折愈合的作用

曾  庆1，黄国志1，梁东辉2，邓虹珠3，易延逵3
  

  1. 南方医科大学附属珠江医院，1康复医学科，2中医科，广东省广州市  510282；3南方医科大学中医药学院，广东省广州市  510515
  • 收稿日期:2016-06-30 出版日期:2016-09-16 发布日期:2016-09-16
  • 通讯作者: 黄国志，博士，主任医师，教授，博士生导师，南方医科大学附属珠江医院康复医学科，广东省广州市 510282
  • 作者简介:曾庆，女，1983年生，湖南省双峰县人，汉族，在职博士，主治医师，主要从事康复医学临床工作。
  • 基金资助:
    广东省科技计划项目(2006B35802005)

Micron traditional Chinese medicine functional lining material: activating blood circulation and promoting fracture healing

Zeng Qing1, Huang Guo-zhi1, Liang Dong-hui2, Deng Hong-zhu3, Yi Yan-kui3
  

  1. 1Department of Rehabilitation Medicine, 2Department of Chinese Medicine, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China; 3School of Chinese Traditional Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2016-06-30 Online:2016-09-16 Published:2016-09-16
  • Contact: Huang Guo-zhi, M.D., Professor, Doctoral supervisor, Chief physician, Department of Rehabilitation Medicine, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Zeng Qing, Studying for doctorate, Attending physician, Department of Rehabilitation Medicine, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • Supported by:
    the Science and Technology Program of Guangdong Province, No. 2006B35802005

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摘要：

文章快速阅读：

 

文题释义：
微米中药：是指采用现代高科技与传统炮制技术和制剂技术相结合而研制的，能保持传统中药固有药效学物质基础的粒度为微米级(平均直径≤15 μm)的新型中药。微米化后的中药因不涉及对原子或分子结构层面上的干预和操作，故药物原有属性、药效特征和功能主治保持不变。同时由于制得的颗粒比表面积增大，能有效增进药物有效成分的溶出，提高药物的生物利用度。
超微粉碎技术：是借助对物料的冲击、碰撞、剪切、研磨、摩擦、分散等手段能把原料加工成微米甚至纳米级的微粉，可使植物细胞破壁率达到90%以上，细胞内有效成分得到充分暴露。超微粉碎技术也可提高药物的靶向性，有助于控制药物在体内的分布等。

背景：课题组研制出一种新型医用骨科外固定材料——微米中药功能衬材，并证实该衬材具有促进碱性磷酸酶分泌，降低血钙，升高血磷和钙磷乘积，促进骨痂形成的作用。
目的：进一步验证微米中药功能衬材促进骨折愈合的疗效。
方法：取新西兰兔24只，造成右侧桡骨骨折模型后随机分组，微米组外敷微米中药功能衬材(微米三七粉混合骨碎补、红花等药物的有效成分)后加石膏外固定，中药对照组外敷普通中药功能衬材(普通三七粉)后加石膏外固定，衬材对照组外敷普通衬材(无菌脱脂棉)后加石膏外固定，模型对照组不予任何治疗。于造模前和造模后第2，4，6周行全血高切黏度、全血中切黏度、全血低切黏度和血浆黏度检测；造模后第6周末处死兔，取桡骨标本进行组织学观察。
结果与结论：①血液流变学指标：微米组全血高切黏度、全血中切黏度、全血低切黏度和血浆黏度均低于同时期其余对照组，造模后第4，6周与模型对照组比较差异有显著性意义(P < 0.05或0.01)；②组织形态学观察：微米组可见大量成骨细胞，编织骨开始塑形，骨髓腔再通；中药对照组可见编织骨、软骨细胞和软骨内成骨共存，断端内、外骨痂相连，骨髓腔未再通；衬材对照组和模型对照组断端内、外骨痂大多尚未相连，骨髓腔未再通；③结果表明：微米中药功能衬材能够活血化瘀，改善微循环，促进成骨细胞增生，并进而促进骨折愈合。

关键词: 生物材料, 骨生物材料, 微米中药, 功能衬材, 骨折, 骨折愈合

Abstract:

BACKGROUND: As a new self-synthetized medical orthopedic fixation material, micron traditional Chinese medicine functional lining material can promote the secretion of alkaline phosphatase and callus formation, decrease serum calcium level, as well as increase serum phosphorus level and calcium-phosphorus product.
OBJECTIVE: To further verify the therapeutic effect of micron traditional Chinese medicine functional lining material on fracture healing.
METHODS: Twenty-four New Zealand rabbits were selected to prepare models of right radial fracture, and then were randomly allocated into four groups: rabbits in micron group were treated with micron traditional Chinese medicine functional lining material (micron Panax notoginseng powder mixed with active ingredients of Davallia divaricata and Carthamus tinctoriousplus) plus plaster external fixation; those in traditional Chinese medicine control group treated with ordinary Chinese medicine functional material plus plaster external fixation; those in lining material control group treated with ordinary lining material (sterile degreasing cotton) plus plaster external fixation; those in model control group received no treatment. The whole blood viscosity at high/middle/low shear rates and plasma viscosity were detected at 2, 4 and 6 weeks before and after modeling. Additionally, rabbits in each group were killed at 6 weeks after modeling, and radial samples were removed for histological observation.
RESULTS AND CONCLUSION: The whole blood viscosity at high/middle/low shear rates and plasma viscosity in the micron group were all lower than those in the other three groups in the same period, and had significant differences from those in the model control group at 4 and 6 weeks after modeling (P < 0.05 or 0.01). A large number of osteoblasts could be found, woven bone began to form and the bone marrow cavity was recanalized in the micron group; in the traditional Chinese medicine group, woven bone, chondrocytes and endochondral ossification coexisted and there was a connection between calluses inside and outside of the broken end, but the bone marrow cavity was not recanalized. In contrast, neither connection between calluses inside and outside of the broken end, nor recanalized bone marrow cavity appeared in the lining material and model control groups. In conclusion, micron traditional Chinese medicine functional lining material can promote fracture healing by improving microcirculation and accelerating osteoblast proliferation.

Key words: Drugs, Chinese Herbal, fractures, Bone, Tissue Engineering

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