中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (34): 5098-5103.doi: 10.3969/j.issn.2095-4344.2016.34.013

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米雄黄对肺癌A549细胞增殖、凋亡及迁移的影响

王巍炜，王德光，巫正伟，张 勇，钱可宝   

  1. 昆明医科大学第三附属医院胸外科，云南省昆明市  650011
  • 收稿日期:2016-06-16 出版日期:2016-08-19 发布日期:2016-08-19
  • 通讯作者: 巫正伟，副主任医师，昆明医科大学第三附属医院胸外科，云南省昆明市 650011
  • 作者简介:王巍炜，男，1979年生，云南省曲靖市人，汉族，博士，副主任医师，主要从事肺癌耐药相关研究。
  • 基金资助:

    云南省科技厅-昆明医科大学联合基金项目(2013FB164)；云南省肿瘤医院博士科研启动基金(BS55201510)

Nano-realgar effect on A549 lung cancer cell proliferation, apoptosis and migration

Wang Wei-wei, Wang De-guang, Wu Zheng-wei, Zhang Yong, Qian Ke-bao   

  1. Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China
  • Received:2016-06-16 Online:2016-08-19 Published:2016-08-19
  • Contact: Wu Zheng-wei, Associate chief physician, Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China
  • About author:Wang Wei-wei, M.D., Associate chief physician, Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China
  • Supported by:

    the Joint Fund Project of Yunnan Provincial Science and Technology Department & Kunming Medical University, No. 2013FB164; Dr. Scientific Research Foundation of Yunnan Cancer Hospital, No. BS55201510

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被引次数

4

摘要：

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文题释义：
纳米雄黄：雄黄可抑制癌症细胞DNA合成，诱导癌症细胞凋亡，提高机体免疫力，发挥抗癌作用。但由于雄黄毒性比较大，溶解性比较低，在临床应用中受到限制。将雄黄制备成纳米雄黄，纳米雄黄的颗粒直径较小，穿透能力较强，生物利用度显著提高，毒性显著降低，在抗肿瘤作用方面有广泛研究。
细胞增殖：是生物体的重要生命特征，细胞以分裂的方式进行增殖。单细胞生物，以细胞分裂的方式产生新的个体。多细胞生物，以细胞分裂的方式产生新的细胞，用来补充体内衰老或死亡的细胞；同时，多细胞生物可以由一个受精卵，经过细胞的分裂和分化，最终发育成一个新的多细胞个体。必须强调指出，通过细胞分裂，可以将复制的遗传物质，平均地分配到2个子细胞中去。可见，细胞增殖是生物体生长、发育、繁殖和遗传的基础。


背景：有研究发现雄黄具有抗肿瘤作用，但关于纳米雄黄对肺癌A549细胞增殖、凋亡及迁移作用的研究不多。
目的：观察纳米雄黄对肺癌A549细胞增殖、凋亡及迁移的影响。
方法：取生长状态良好的肺癌A549细胞悬液，接种于6孔板，实验组加入100 mg/L纳米雄黄4 µL，对照组加入等量生理盐水。培养48 h，显微镜下观察细胞生长；培养24，48，72 h，MTT法检测细胞增殖；培养24，48 h，流式细胞仪检测细胞凋亡；培养24 h，免疫组织化学法检测整合素β1和上皮钙黏素表达。
结果与结论：①细胞生长：实验组细胞体积缩小变形，数目减少，形状变为圆形或椭圆形，细胞呈散在生长；对照组细胞贴壁生长，体积较大，胞浆比价丰富，形状呈梭形，生长比较旺盛，大小均匀，部分细胞融合成片；②细胞增殖：实验组不同时间点的A值低于对照组(P < 0.05)，不同时间点的细胞生长抑制率高于对照组(P < 0.05)；③细胞凋亡：实验组晚期凋亡率、总细胞凋亡率明显高于对照组(P < 0.05)；④免疫组织化学检测结果：实验组细胞整合素β1阳性细胞面密度、上皮钙黏素阳性细胞面密度明显低于对照组(P < 0.05)；⑤结果表明：纳米雄黄可抑制肺癌A549细胞的增殖、侵袭和转移，诱导其凋亡。

ORCID: 0000-0001-7874-1421(王巍炜)

关键词: 生物材料, 纳米材料, 纳米雄黄, 肺癌, A549细胞, 增殖, 凋亡, 细胞生长, 整合素β, 上皮钙黏素

Abstract:

BACKGROUND: Studies have found that realgar has anti-tumor effect, but its effect on proliferation, apoptosis and migration of A549 lung cancer cells is rarely reported.
OBJECTIVE: To study the effect of nano-realgar on A549 lung cancer cell proliferation, apoptosis and migration.
METHODS: Cultured A549 lung cancer cells were seeded onto 6-well culture plates containing 100 mg/L nano-realgar (experimental group) or normal saline (control group). Cell growth was observed under microscope for 48 hours. Cell proliferation and apoptosis were detected using MTT assay (24, 48, 72 hours of culture) and flow cytometry (24 and 48 hours of culture), respectively. Integrin β1 and cadherin expression was detected using immunohistochemical method at 24 hours of culture.
RESULTS AND CONCLUSION: (1) Cell growth. In the experimental group, shrunk cells that were round or oval-shaped were scattered, and cell number was reduced. In the control group, adherent cells characterized by big size, abundant cytoplasm and fusiform shape grew vigorously. Moreover, some cells were fused into pieces. (2) Cell proliferation. The absorbance values of cells were significantly lower in the experimental group than the control group at different time (P < 0.05), while the cell growth inhibition ratio was higher in the experimental group than the control group (P < 0.05). (3) Cell apoptosis. The end-stage apoptotic rate and total apoptotic rate of cells were both higher in the experimental group than the control group (P < 0.05). (4) Immunohistochemical detection. The A549 cell surface integrin β1 and cadherin positive densities were significantly lower in the experimental group than the control group (P < 0.05). Taken together, the nano-realgar can inhibit the proliferation, invasion and metastasis, and induce apoptosis in A549 cells.

Key words: Nanostructures, Lung Neoplasms, Cell Proliferation, Apoptosis

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