中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (28): 4234-4242.doi: 10.3969/j.issn.2095-4344.2016.28.019

• 干细胞综述 stem cell review • 上一篇    下一篇

不同预处理策略促进间充质干细胞的归巢

王国任，白志明   

  1. 中南大学湘雅医学院附属海口医院泌尿外科，海南省海口市  570208
  • 修回日期:2016-04-06 出版日期:2016-07-01 发布日期:2016-07-01
  • 通讯作者: 白志明，博士，主任医师，教授，博士生导师，中南大学湘雅医学院附属海口医院泌尿外科，海南省海口市 570208
  • 作者简介:王国任，男，1990年生，湖南省衡阳县人，汉族，中南大学湘雅医学院附属海口医院泌尿外科在读硕士，医师，主要从事干细胞对肾脏损伤修复效应方面的研究。
  • 基金资助:

    海南省自然科学基金(811151)

Preconditioning strategies for promoting mesenchymal stem cell homing

Wang Guo-ren, Bai Zhi-ming   

  1. Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • Revised:2016-04-06 Online:2016-07-01 Published:2016-07-01
  • Contact: Bai Zhi-ming, M.D., Chief physician, Professor, Doctoral supervisor, Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • About author:Wang Guo-ren, Studying for master’s degree, Physician, Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • Supported by:

    the Natural Science Foundation of Hainan Province, China, No. 811151

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被引次数

1

摘要：

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文题释义：
归巢：是干细胞作用的初始环节，亦是关键环节，通常指间充质干细胞移植后向受损组织迁移，进而定位于目标位置。Karp等将归巢定义为：干细胞在目标组织的脉管系统内被捕获，进而跨内皮迁移至靶组织的过程。归巢过程相当复杂，涉及一系列的趋化因子及其受体、细胞因子、生长因子、蛋白酶、黏附分子及细胞外基质分子。
不同预处理策略：指移植间充质干细胞前对靶组织或间充质干细胞进行预处理的不同方法，处理方式包括：趋化因子注射、物理效应(低氧处理、医用冲击波、超声波和/或超声微泡、激光、细胞因子或化学物质)、细胞基因修饰、细胞表面修饰、3D培养细胞技术等，并根据预处理对象不同可分为组织预处理和细胞预处理。

 

摘要
背景：归巢是干细胞发挥组织修复作用的初始及关键环节。目前研究表明骨髓间充质干细胞无法高效地归巢至靶组织是影响修复效果的主要障碍。预处理策略为促进干细胞归巢提供了新思路。
目的：对促进间充质干细胞归巢的预处理策略进行综述。
方法：以“stem cell, mesenchymal stem cells, preconditioning, homing, migration”为检索词按不同组合在PubMed数据库中检索2000年1月至2015年9月关于预处理策略促进间充质干细胞归巢的文章，根据纳入标准，最终选择72篇文章进行综述。
结果与结论：移植间充质干细胞前对靶组织或间充质干细胞进行预处理，可显著促进其归巢至受损组织从而增强组织修复效应。组织预处理策略正是通过影响局部微环境，达到上调趋化因子表达等效果，从而促进间充质干细胞归巢。间充质干细胞预处理策略，例如基因修饰、细胞因子诱导等，主要以间充质干细胞表面趋化因子受体为效应器，通过上调其表达来促进靶向归巢。预处理策略将为干细胞治疗应用于临床带来了极大希望。

 

 

关键词: 干细胞, 移植, 预处理策略, 组织预处理, 细胞预处理, 充质干细胞, 移植, 归巢, 组织修复, 海南省自然科学基金

Abstract:

BACKGROUND: Homing is the initial and key procedure of stem cells-based tissue restoration. Current studies have shown that the inability to recruit bone marrow mesenchymal stem cells to target tissue with high efficiency remains a significant barrier to tissue restoration. Preconditioning strategy provides a new insight to promote stem cell homing.
OBJECTIVE: To review preconditioning strategies for promoting the homing of stem cells.
METHODS: In PubMed database, different combinations of terms from “stem cell, mesenchymal stem cells, preconditioning, homing, migration” served as search terms to retrieve articles referring preconditioning strategies for promoting mesenchymal stem cell homing published from January 2000 to September 2015. According to the inclusion criteria, 72 articles were selected for final review.
RESULTS AND CONCLUSION: Pretreating target tissue or mesenchymal stem cells ahead of cell transplantation, known as tissue preconditioning or cell preconditioning, prominently promotes the homing of mesenchymal stem cells, therefore enhancing tissue restoration effect. Tissue preconditioning is designed to up-regulate expression of chemokines by varying the local microenvironment, thereby increasing homing ability of mesechymal stem cells. Mesenchymal stem cell preconditioning strategies, for example, gene modification and cytokine induction, are mainly to up-regulate expression of chemokine receptors on the surface of mesenchymal stem cells as effectors, and thus promote targeted cell homing. Overall, preconditioning strategy will bring great hope to apply stem cell therapy into the clinic.

 

 

Key words: Mesenchymal Stem Cells, Transplantation, Tissue Engineering

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