中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (16): 2369-2375.doi: 10.3969/j.issn.2095-4344.2016.16.012

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

SonicFill超声树脂对大鼠成牙本质细胞和牙髓微血管的影响

赵千宁,王健平,佟玮玮,苏 丹,余彦衡   

  1. 佳木斯大学附属口腔医院,黑龙江省佳木斯市  154002
  • 收稿日期:2016-02-07 出版日期:2016-04-15 发布日期:2016-04-15
  • 通讯作者: 王健平,主任医师,黑龙江省佳木斯大学附属口腔医院牙体牙髓病科,黑龙江省佳木斯市154002
  • 作者简介:赵千宁,女,1990年生,黑龙江省齐齐哈尔市人,汉族,佳木斯大学在读硕士,执业医师,主要从事牙体牙髓病学研究。
  • 基金资助:

    佳木斯大学研究生科技创新项目(LM2015-076)

Effect of SonicFill ultrasonic resin on rat odontoblasts and dental pulp vessels

Zhao Qian-ning, Wang Jian-ping, Tong Wei-wei, Su Dan, Yu Yan-heng   

  1. Affiliated Stomatology Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • Received:2016-02-07 Online:2016-04-15 Published:2016-04-15
  • Contact: Wang Jian-ping, Chief physician, Affiliated Stomatology Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • About author:Zhao Qian-ning, Studying for master’s degree, Physician, Affiliated Stomatology Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • Supported by:

    Science and Technology Innovation Projects for Graduate Students in Jiamusi University, No. LM2015-076

摘要:

文章快速阅读:

 

文题释义:
SonicFill超声树脂:通过Kavo手机输送树脂一次性充填,省略手动分层充填,简化了医生的操作,一次固化的最大深度可达5 mm,改变了传统的后牙充填方式。它的原理是通过高频超声波能量打破树脂中的颗粒联系,使树脂的黏度下降了87%,增加了树脂的流动性,使树脂更加快速、均匀和精确地充满整个窝洞,减少因气泡而产生的空腔。当超声波能量停止时,树脂恢复可塑性,变的黏稠,这种状态更利于雕刻和塑形。基质的主要成分为双酚A二甲基丙烯酸缩水甘油酯、双酚A二甲基丙烯酸乙酯、二甲基丙烯酸二缩三乙二醇酯等,填料组成为玻璃离子、二氧化硅、钡、铝、硼、硅酸盐等化学成分,其含量占83.5%,体积占69%。与传统树脂相比,SonicFill超声树脂改良了填料颗粒,降低了聚合收缩率,减小了热膨胀系数,放置时拥有流体树脂的连贯性,使得充填更充分,固化后又能达到充填材料的高硬度,使得充填后更耐磨。
光固化复合树脂修复牙体时的牙髓反应:与很多因素有关,一方面,龋病、磨损、备洞所产生的高温、酸蚀的刺激、光固化灯照射产热等因素都可能产生一定的刺激;另一方面,光固化复合树脂本身也可对牙髓产生影响,就光固化复合树脂的成分而言,不同复合树脂的填料也不同,有些树脂自身就包含微量有害物质,也可能会引起牙髓的急性炎症,其中低分子有机树脂成分会通过牙本质小管对牙髓产生刺激;另一方面,光固化复合树脂充填后,由于充填材料的聚合收缩,可能会有微渗漏的情况发生,而微渗漏会使得细菌容易侵入,这些的原因综合起来,可能会引起牙髓急性炎症之后转变为慢性牙髓炎,最后炎症减弱或痊愈。
 
背景:SonicFill超声树脂在机械性能上优于传统光固化复合树脂充填材料,但其对牙髓成牙本质细胞和牙髓微血管的影响目前尚不清楚。
目的:通过组织学方法观察SonicFill超声树脂对大鼠牙髓成牙本质细胞及牙髓微血管的影响。
方法:实验选择Wistar大鼠25只,在大鼠的第一和第二磨牙中选取96颗牙齿作为研究对象,随机分为2组,SonicFill超声树脂组和Filtek Z350纳米树脂组,每组48颗实验牙,分为7 d和30 d两个实验周期。另外随机选取5颗非实验牙,不干预,作为正常空白对照。选择上颌第一、二磨牙牙合面制备Ⅰ类窝洞,分别采用SonicFill超声树脂和Filtek Z350纳米树脂进行填充。于充填后7,30 d,墨汁灌注处死大鼠,取牙齿标本,观察牙髓的成牙本质细胞及牙髓微血管的扩张。
结果与结论:①充填后7 d:两组均出现相似的牙髓组织改变,表现为牙髓轻度炎症反应,部分标本可见接近洞底牙髓组织中散在的炎症细胞浸润,多数标本可见成牙本质细胞排列紊乱,偶见空泡变性,牙髓微血管有扩张现象。②充填后30 d:SonicFill超声树脂组成牙本质细胞排列基本恢复正常,炎性反应消退,牙髓微血管无扩张,基本恢复正常;Filtek Z350纳米树脂组成牙本质细胞排列紊乱现象减少,炎性反应减轻,牙髓微血管扩张增粗现象减少。③结果表明,两种树脂对成牙本质细胞和牙髓微血管都 会产生一定的影响,所产生的影响相似且都较小。
中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程
ORCID: 0000-0002-5993-3767(赵千宁)

关键词: 生物材料, 口腔生物材料, SonicFill超声树脂, Filtek Z350纳米树脂, Wistar大鼠模型, 成牙本质细胞, 牙髓微血管

Abstract:

BACKGROUND: The mechanical properties of SonicFill ultrasonic resin are better than those of the traditional light-cured composite resin, but its effect on odontoblasts and dental pulp vessels is unclear.

OBJECTIVE: To study the effect of SonicFill ultrasonic resin on odontoblasts and dental pulp vessels of rats using histological methods.
METHODS: Twenty-five Wistar rats are selected for experiment. Ninety-six teeth from the first and second molars were selected and randomly divided into SonicFill ultrasound resin group and Filtek Z350 nano resin group with 48 teeth in each group. Experimental teeth in each group were equally subdivided into 7 days group and 30 days group. Besides, randomly selected five teeth with no intervention served as normal control group. After anesthesia and disinfection, class I cavities on the occlusal surface of the maxillary first and second molars were prepared and filled with SonicFill ultrasound resin and Filtek Z350 nano resin group, respectively. The rats were sacrificed by ink perfusion method, and tooth specimens were taken to observe the changes of odontoblasts and pulp microvessels.
RESULTS AND CONCLUSION: At 7 days after filling, two groups had similar changes in the pulp presenting with mild inflammation. Besides, there were scattered inflammatory cells distributing in the cave pulp tissue, and in most of the specimens, odontoblasts arranged disorderly with little vacuoles and microvascular dilatation in the pulp. At 30 days after filling, in the SonicFill ultrasonic resin group, odontoblasts arranged orderly, inflammatory reaction and microvascular dilatation disappeared basically. In contrast, in the Filtek Z350 nano resin group, disordered odontoblasts decreased, inflammatory reaction weakened, as well as microvascular dilatation became less. These results show that under the experimental condition, both of the SonicFill ultrasonic resin and Filtek SonicFill Z350 nano resin play certain roles in the formation of odontoblasts and dental pulp vessels, but over time all of these effects can automatically disappear. 
中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Rats, Wistar, Self-Curing of Dental Resins, Tissue engineering