中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (12): 1697-1703.doi: 10.3969/j.issn.2095-4344.2016.12.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

海螵蛸/外消旋聚乳酸复合人工骨在动物体内的降解及相容性

覃广兵1，2，肖 颖1，王 伟2，肖荣驰1，欧 俊3   

  1. 1桂林医学院附属医院脊柱骨病外科，广西壮族自治区桂林市 541000；2桂林医学院，广西壮族自治区桂林市 541000；3桂林理工大学，广西壮族自治区桂林市 541000
  • 收稿日期:2016-01-10 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 肖颖，副主任医师，硕士生导师，桂林医学院附属医院脊柱骨病外科，广西壮族自治区桂林市 541000
  • 作者简介:覃广兵，男，1986年生，广西壮族自治区河池市东兰县人，壮族，桂林医学院在读硕士，主要从事脊柱骨病、骨与关节损伤方面的基础及临床研究。

Degradation and compatibility of cuttlebone/racemic polylactic acid composite artificial bone in animals

Qin Guang-bing1,2, Xiao Ying1, Wang Wei2, Xiao Rong-chi1, Ou Jun3   

  1. 1Department of Spinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China; 2Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China; 3Guilin University of Technology, Guilin 541000, Guangxi Zhuang Autonomous Region, China
  • Received:2016-01-10 Online:2016-03-18 Published:2016-03-18
  • Contact: Xiao Ying, Associate chief physician, Master’s supervisor, Department of Spinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China
  • About author:Qin Guang-bing, Studying for master’s degree, Department of Spinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China; Guilin Medical University, Guilin 541000, Guangxi Zhuang Autonomous Region, China

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海螵蛸：主要含有碳酸钙及少量的锶、镁、铝、镍等金属元素，具有天然、规则的壁龛样网格结构，孔隙率达90%左右，不论从孔径的大小、形状还是孔隙率来看，海螵蛸的天然网格结构都比较理想，具有良好的生物学活性，但其降解却是一个相当长的过程。
外消旋聚乳酸：属于脂肪族聚酯类高分子材料，它在微生物、水、酸等的作用下可完全降解为水和二氧化碳。但在具有良好生物相容性和生物安全性的同时，外消旋聚乳酸也存在热稳定性较差、力学性能偏低及完全绝缘等缺点。

 

背景：为改善海螵蛸作为骨修复材料时降解不完全问题，前期研究制备了海螵蛸/外消旋聚乳酸复合人工骨材料。
目的：观察海螵蛸/外消旋聚乳酸复合人工骨在动物体内的降解及生物相容性。
方法：将30只新西兰大白兔随机分为4组，制作右侧桡骨缺损区，实验组于骨缺损处置入海螵蛸/外消旋聚乳酸复合人工骨块，并在靠上方外侧肌和直肌肌囊内置入海螵蛸/外消旋聚乳酸复合人工骨片；对照1组于骨缺损处置入海螵蛸块，并在靠上方外侧肌和直肌肌囊内置入海螵蛸片；对照2组于骨缺损处置入外消旋聚乳酸块，并在靠上方外侧肌和直肌肌囊内置入外消旋聚乳酸片；空白对照组不置入任何材料。术后2，4，8周进行X射线与组织学检查。
结果与结论：①骨缺损置入实验：实验组术后4，8周的骨密度高于对照组1组、对照2组、空白对照组(P < 0.05)，术后不同时间点的骨矿化沉积率高于对照1组、对照2组、空白对照组(P < 0.05)。术后8周时，实验组骨组织由两端周围向材料中央方向生长，形成多处骨岛状结构，材料残存较少，骨髓腔与材料呈相交通状；对照1组残存材料较多，骨髓腔与材料未交通；对照2组残存材料较多，骨髓腔与材料未交通。②肌囊埋置实验：3种材料置入后2周材料周围炎症细胞较多，术后4周减轻，术后8周基本消失，材料部分降解。结果表明海螵蛸/外消旋聚乳酸复合人工骨具有良好的生物相容性及降解性。 

关键词: 生物材料, 骨生物材料, 海螵蛸, 外消旋聚乳酸, 复合人工骨, 骨缺损修复, 降解, 相容性

Abstract:

BACKGROUND: Cuttlebone/racemic polylactic acid composite artificial bone has been prepared in the previous studies to improve the incomplete degradation of cuttlebone.
OBJECTIVE: To observe the degradation and biocompatibility of cuttlebone/racemic polylactic acid composite artificial bone in animals.
METHODS: Thirty healthy New Zealand white rabbits were randomly divided into four groups. Models of right radial defects were prepared in rabbits, and model rabbits were subjected to implantation of cuttlebone/racemic polylactic acid composite artificial bone into the defects and muscular sac between the radial lateralis muscle and rectus (experimental group), implantation of cuttlebone into the defects and muscular sac between the radial lateralis muscle and rectus (control group 1), implantation of racemic polylactic acid into the defects and muscular sac between the radial lateralis muscle and rectus (control group 2), or no treatment (blank control group), respectively. At 2, 4, 8 weeks after operation, X-ray and histological examinations were performed in the four groups.
RESULTS AND CONCLUSION: (1) Compared with the other three groups, the bone mineral density of the experimental group was significantly higher at 4 and 8 weeks after material implantation into the defects (P < 0.05), and moreover, the bone mineral apposition rate of the experimental group was significantly higher at different time after operation (P < 0.05). At 8 weeks after operation, the bone tissues in the experimental group grew from the both ends to the center to form multiple bone island-like structures, with less residual materials, and the marrow cavity and implanting material were in a traffic manner; in the control group 1, there were many residual materials, and no intercommunication was found between the marrow cavity and implant material. (2) At 2 weeks after material implantation into the muscle capsule, there were more inflammatory cells, but the inflammation relieved at 4 weeks and disappeared basically at 8 weeks, and the material was degraded partially. These findings indicate that the cuttlebone/racemic polylactic acid composite artificial bone is a kind of good bone substitute material that has good biocompatibility and degradability.
 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程