中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (12): 1704-1710.doi: 10.3969/j.issn.2095-4344.2016.12.005

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

锌修饰硅酸钙陶瓷涂层与骨整合

徐立璋1，叶晓健1，李 恺2，郑学斌2，唐 峰1，许 鹏1，席焱海1，许国华1，侯春林1，余将明1   

  1. 1解放军第二军医大学附属长征医院骨科，上海市 200003；2中国科学院上海市硅酸盐研究所，上海市 200050
  • 收稿日期:2016-01-11 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 余将明，主治医师，解放军第二军医大学附属长征医院骨科，上海市 200003
  • 作者简介:徐立璋，男，1986年生，福建省浦城市人，汉族，解放军第二军医大学在读硕士，主要从事脊柱外科研究。
  • 基金资助:
    国家自然科学基金资助项目(81301537)；国家高技术研究发展计划(863)资助项目(2013AA032203)

Zinc-modified calcium silicate bioceramics coating and osteointegration

Xu Li-zhang1, Ye Xiao-jian1, Li Kai2, Zheng Xue-bin2, Tang Feng1, Xu PengXi1 , Xu Guo-hua1, Hou Chun-lin1, Yu Jiang-ming1   

  1. 1Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
  • Received:2016-01-11 Online:2016-03-18 Published:2016-03-18
  • Contact: Yu Jiang-ming, Attending physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • About author:Xu Li-zhang, Studying for master’s degree, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81301537; the National High Technology Research and Development Program of China (863 Program), No. 2013AA032203

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摘要：

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文题释义：

硅酸钙陶瓷：以硝酸钙和硅酸钠为原料，采用化学共沉淀法合成硅酸钙粉体，对粉体进行烧结，即可制得硅酸钙陶瓷。硅酸钙陶瓷涂层具有良好的生物活性，能与宿主骨形成很好整合，但机械强度低、化学稳定性差，容易降解和吸收，且随着浸泡时间的延长，涂层与基体间的结合强度明显降低。
骨整合：在光学显微镜下，植入体与骨组织之间呈现的无纤维结缔组织界面层的直接有序的结构和功能连接，又称骨性结合，可有效地分散合力，保证种植体的稳固。

 

背景：前期实验在钛基材表面制备了锌修饰硅酸钙陶瓷涂层，具有良好的化学稳定性及抗菌性能。
目的：进一步观察锌修饰硅酸钙陶瓷涂层对骨整合的影响。
方法：将锌修饰硅酸钙陶瓷涂层钛片(实验组)、硅酸钙陶瓷涂层钛片(对照组)及纯钛片(空白对照组)分别与前成骨细胞MC3T3-E1共同培养，检测细胞的黏附、增殖、钙化及Ⅰ型胶原和骨钙素表达。将上述3组材料分别置入新西兰大白兔股骨内，置入后1.5个月观察骨质增生及植入体与宿主骨整合情况。

结果与结论：①体外实验：培养12 h，实验组黏附细胞数量多于对照组、空白对照组(P < 0.05)；培养14 d，实验组细胞增殖活性、钙结节形成能力强于对照组、空白对照组(P < 0.05)；培养21 d，3组细胞Ⅰ型胶原表达无差异，但实验组骨钙素表达高于对照组、空白对照组(P < 0.05)。②体内实验：实验组骨质增生明显，涂层材料与骨界面直接接触，界面间可见新骨生成，纤维组织分隔较少；对照组可见少量骨质增生，空白对照组几乎不见骨质增生，且两组植入体表面小部分区域与宿主骨接触，大部分区域与周围的骨小梁之间有纤维组织分隔。③结果表明，锌修饰硅酸钙陶瓷涂层可促进钛种植体与宿主骨整合。 

ORCID: 0000-0002-7537-8302(余将明)

关键词: 生物材料, 骨生物材料, 锌修饰硅酸钙陶瓷, 涂层, MC3T3-E1细胞, 成骨分化, 矿化, 骨整合, 国家自然科学基金

Abstract:

BACKGROUND: Zinc-modified calcium silicate (CaSiO3) bioceramics coating on the titanium surface prepared in preliminary experiments has good chemical stability and antibacterial property.
OBJECTIVE: To observe the effects of zinc-modified CaSiO3 bioceramics coating on osteointegration.
METHODS: MC3T3-E1 cells were respectively cultured on the titanium with zinc-modified CaSiO3 bioceramics coating (experiment group), titanium with CaSiO3 bioceramics coating (control group) and pure titanium (blank control group). Then, cell adhesion, proliferation, calcification rate and the expression of type I collagen and osteocalcin were detected. The implant materials mentioned above were respectively inserted into the femurs of New Zealand white rabbits, and after 1.5 months, the osteoproliferation and osteointegration between the implants and the host were tested.
RESULTS AND CONCLUSION: In vitro experiment: The number of adhesive cells at 12 hours after co-culture was significantly increased in the experimental group compared with the control group and blank control group (P < 0.05). At 14 days after co-culture, cell proliferation ability and ability of calcium nodule formation in the experiment group were significantly better than those in the other groups (P < 0.05). At 21 days after co-culture, there was no significant difference in the expression of type I collagen, but the expression of osteocalcin in the experiment group was higher than that in the control group and blank control group (P < 0.05). In vivo experiment: In the experiment group, a large amount of bone substances were detected, the coating materials directly contacted with the bone interface, new bone tissues and little fibrous tissues were observed at the interface. In contrast, there was a small amount of bone hyperplasia in the control group and almost no bone hyperplase in the blank control group. Moreover, a small part of the implant directly contacted with the bone interface and the most part was separated from bone trabeculae by fibrous tissues. These findings indicate that zinc-modified CaSiO3 bioceramics coating can enhance the ability of osteointegration between titanium implants and the host.