中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (6): 868-875.doi: 10.3969/j.issn.2095-4344.2016.06.016

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞移植联合氯化锂治疗兔股骨头缺血坏死疗效

李 巍1，卢小伟2，冼 呈1，劳 山1   

  1. 1广西医科大学第一附属医院骨关节外科，广西壮族自治区南宁市  530021；2河南省安阳市第三人民医院外二一科，河南省安阳市  455000
  • 收稿日期:2016-01-02 出版日期:2016-02-05 发布日期:2016-02-05
  • 通讯作者: 劳山，博士，硕士生导师，主任医师，广西医科大学第一附属医院骨关节外科，广西壮族自治区南宁市 530021
  • 作者简介:李巍，男，1984年生，广西壮族自治区南宁市人，汉族，2011年广西医科大学毕业，硕士，主治医师，主要从事骨坏死与骨关节退行性疾病的发病机制及修复研究。
  • 基金资助:
    广西医疗卫生重点科研课题(重2010014)

Bone marrow mesenchymal stem cell transplantation combined with lithium chloride treatment for avascular necrosis of the femoral head in rabbits

Li Wei1, Lu Xiao-wei2, Xian Cheng1, Lao Shan1   

  1. 1Department of Bone and Joint Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; 2Department of Surgery, Third People’s Hospital of Anyang, Anyang 455000, Henan Province, China
  • Received:2016-01-02 Online:2016-02-05 Published:2016-02-05
  • Contact: Lao Shan, M.D., Master’s supervisor, Chief physician, Department of Bone and Joint Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Li Wei, Master, Attending physician, Department of Bone and Joint Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Medical Treatment and Public Health Project of Guangxi Province, No. 2010014

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530

被引次数

4

摘要：

文章快速阅读：

文题释义：

骨髓基质干细胞：骨髓基质是指为造血系统提供的微环境，起支持作用的结题组织，可分为细胞和细胞外基质成分，其中细胞就是骨髓基质细胞，其间存在着间充质细胞，总数仅占十万分之一。

氯化锂：为GSK-3β的抑制剂，可使GSK-3β的底物β-catenin免受降解，刺激骨髓间质干细胞增殖并向成骨细胞分化，增加骨形成率，使成骨细胞数量明显增加。

 

背景：氯化锂促进骨髓间充质干细胞增殖并促进股骨头缺血坏死区成骨能力的研究日益受到关注。
目的：比较移植骨髓间充质干细胞联合氯化锂修复股骨头坏死外的优势。
方法：①取出生1周的新西兰白兔第2代的骨髓间充质干细胞，以加入含0，5，10，20，40 mmol/L氯化锂培养。②选取48只健成年新西兰大白兔，用液氮冷冻方法制成右侧股骨头坏死模型，随机分为4组，单纯造模组：不植入任何材料；氯化锂组：造模3 d后每日应用氯化锂；骨髓间充质干细胞移植组：造模后用明胶海绵植入股骨头内，制成骨髓间充质干细胞悬液注入股骨头内；骨髓间充质干细胞移植+氯化锂组：在骨髓间充质干细胞移植组的基础上每日应用氯化锂；氯化锂的用法为每天45.2 mg/kg腹腔注射，术后3 d开始，连续应用4周。

结果与结论：①以10 mmol/L的浓度对进兔骨髓间充质干细胞的增殖起最大作用，超过10 mmol/L的氯化锂促进作用开始下降。②股骨头坏死模型兔经干细胞联合氯化锂治疗后，形态有所恢复，骨密度增加，骨小梁增粗，股骨头中β-连环蛋白表达水平增加，且效果优于单纯干细胞或氯化锂治疗。③说明骨髓间充质干细胞移植联合氯化锂可以促进股骨头坏死区骨小梁的增生骨量增加骨密度增高，促进股骨头坏死的修复。 

ORCID: 0000-0002-1649-5787 (李巍)

关键词: 干细胞, 移植, 氯化锂, 骨髓间充质干细胞, β-连环蛋白, 股骨头坏死, 兔, Wnt信号通路, 骨小梁

Abstract:

BACKGROUND: Lithium chloride can promote the proliferation and osteogenic capacity of bone marrow mesenchymal stem cells in the necrotic region after avascular necrosis of the femoral head, which has become an issue of concern.
OBJECTIVE: To compare the advantages and disadvantages of bone marrow stem cell transplantation combined with lithium chloride in the treatment of rabbit femoral head necrosis.
METHODS: Passage 2 bone marrow mesenchymal stem cells from 1-week-old New Zealand rabbits were cultured in 0, 5, 10, 20, 40 mmol/L lithium chloride. Forty-eight healthy adult New Zealand rabbits were selected to make femoral head necrosis models in the right femoral head using liquid nitrogen freezing method and then randomized into four groups: model group with no implantation; lithium chloride group given lithium chloride treatment at 3 days after modeling; cell transplantation group given gelatin sponge implantation and bone marrow mesenchymal stem cell suspension injection into the femoral head after modeling; combined group given bone marrow mesenchymal stem cell suspension injection and lithium chloride treatment. Intraperitoneal injection of lithium chloride (45.2 mg/kg) was given daily beginning at the postoperative 3rd day, and the treatment duration was 4 weeks.
RESULTS AND CONCLUSION: Lithium chloride at 10 mmol/L had the maximum effect on the proliferation of rabbit bone marrow mesenchymal stem cells, and if the concentration of lithium chloride was > 10 mmol/L, the promotion role of lithium chloride began to decline. After combined treatment, the morphology of the femoral head was restored a little, with increased bone density and thickened trabecular bone; the level of β-catenin in the femoral head was significantly increased in the combined group compared with the cell transplantation group or the lithium chloride group. These findings show that bone marrow stem cell transplantation combined with lithium chloride treatment can promote the recovery from femoral head necrosis by increasing bone mass of the trabecular bone and bone density of the femoral head in the necrotic region.