关键词: 实验动物模型, 心肺及血管损伤动物模型, 哮喘, DO11.10小鼠, BALB/c小鼠, 卵清蛋白, 致敏, 激发, 气道反应, 炎症, 国家自然科学基金

Abstract:

BACKGROUND: The incidence of asthma increases year by year. Therefore, establishment and improvement of optimized animal models of asthma are important for the investigation of asthma. 
OBJECTIVE: To induce asthma method in Balb/c and DO11.10 mice and to compare their difference. 
METHODS: A total of 24 Balb/c mice were equally and randomly divided into groups A-D and 24 DO11.10 mice were equally divided into groups E-H. Mice in the groups A and E were induced to sensitization using PBS on day 1, and atomization using PBS on days 8-11. Mice in the groups B and F were induced to sensitization using ovalbumin on day 1, and atomization using ovalbumin on days 8-11. Mice in the groups C and G were induced to sensitization using PBS on days 1, 7 and 14, and atomization by PBS on days 22-25. Mice in the groups D and H were induced to sensitization using ovalbumin on days 1, 7 and 14, and atomization using ovalbumin on days 22-25. 
RESULTS AND CONCLUSION: One time of sensitization and four times of atomization only caused mild inflammatory reaction in the lungs in Balb/c mice. Three times of sensitization and 4 times of atomization caused clear airway hyperreactivity, inflammatory cell infiltration in lung, increased mucus secretion in bronchial goblet epithelium in Balb/c mice. Simultaneously, the number of inflammatory cells and eosinophils significantly increased in bronchoalveolar lavage fluid. Th2 type cytokine levels also increased in the bronchoalveolar lavage fluid. In DO11.10 mice, one time of sensitization and four times of atomization induced significant inflammatory response. Specially, the number of neutrophils obviously increased in the bronchoalveolar lavage fluid. However, after three times of sensitization and four times of atomization, inflammatory reaction significantly decreased in DO11.10 mice. These findings indicate that asthma model could be successfully induced with ovalbum in both Balb/c and DO11.10 mice. There were some differences in the induction and pathological features for asthma model between the two species of mice.