中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (47): 7603-7607.doi: 10.3969/j.issn.2095-4344.2015.47.010

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

咬合刺激调控胶原骨粉修复骨缺损过程中骨改建的形态学分析

茅传青，王 锦，王承勇，卢 萌，陈伟辉   

  1. 福建医科大学附属协和医院口腔颌面外科，福建省福州市 350001
  • 收稿日期:2015-09-09 出版日期:2015-11-19 发布日期:2015-11-19
  • 通讯作者: 陈伟辉，副教授，主任医师，福建医科大学附属协和医院口腔颌面外科，福建省福州市350001
  • 作者简介:茅传青，男，1988年生，福建省三明市人，汉族，硕士，医师，主要从事牙种植与基础临床研究。
  • 基金资助:

    福建省自然基金杰出青年项目(2012J06017)；国家自然科学基金(31070838)；福建省卫生厅青年基金(2012-1-20)

Occlusal stimulation regulates bone remodeling during bone defect repair using collagen substitutes: a morphological analysis

Mao Chuan-qing, Wang Jin, Wang Cheng-yong, Lu Meng, Chen Wei-hui   

  1. Department of Oral and Maxillofacial Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China
  • Received:2015-09-09 Online:2015-11-19 Published:2015-11-19
  • Contact: Chen Wei-hui, Associate professor, Chief physician, Department of Oral and Maxillofacial Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China
  • About author:Mao Chuan-qing, Master, Physician, Department of Oral and Maxillofacial Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China
  • Supported by:

    the Natural Science Foundation for the Outstanding Youth of Fujian Province, China, No. 2012J06017; the National Natural Science Foundation of China, No. 31070838; the Youth Foundation of Fujian Provincial Health Department of China, No. 2012-1-20

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摘要：

背景：咬合刺激对于颌骨的功能及改建至关重要，但人们对其调节骨移植修复骨缺损过程中骨改建的作用尚缺少清楚的认识。
目的：应用形态学方法分析咬合刺激对胶原骨粉修复骨缺损中骨改建的可能调控作用。
方法：在成年SD大鼠左侧下颌骨及顶骨区分别建立标准骨缺损模型，随即植入胶原骨粉充填骨缺损区。术后12周，通过X射线、苏木精-伊红染色、Gomori染色、抗酒石酸酸性磷酸酶染色及骨形成蛋白2免疫组织化学染色观察两骨缺损区的差异。
结果与结论：下颌骨及顶骨骨缺损区均有新骨形成，顶骨骨缺损区的板层骨形成量明显多于下颌骨骨缺损区，新骨形成矿化度明显高于下颌骨骨缺损区，说明顶骨骨缺损区的骨改建优于下颌骨骨缺损区；顶骨骨缺损区抗酒石酸酸性磷酸酶及骨形成蛋白2积分吸光度值均低于下颌骨骨缺损区，说明顶骨骨缺损区的破骨细胞及成骨细胞活性低于下颌骨骨缺损区。表明咬合刺激可能通过影响骨的矿化及成熟而导致下颌骨骨缺损修复过程中骨改建的延迟。
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 生物材料, 骨生物材料, 胶原骨粉, 顶骨, 下颌骨, 骨改建, 咬合刺激, 国家自然科学基金

Abstract:

BACKGROUND: Occlusal stimulation is essential for mandible function and remodeling, but there is still a lack of clear understanding about the effect of occlusal stimulation on the bone remodeling in the process of bone defect repair using bone grafts.

OBJECTIVE: To analyze the possible regulative effect of occlusal stimulation on bone remodeling in the process of bone defect repair using collagen substitutes.
METHODS: Standard models of bone defects were respectively established in left mandible and parietal bone area of adult Sprague-Dawley rats. Then the bone defects area were filled with collagen and bone meal. The differences of two bone defects areas were observed by X-ray, hematoxylin-eosin staining, Gomori staining, tartrate-resistant acid phosphatase staining and bone morphogenetic protein 2 immunohistochemical staining at the 12th week after operation.
RESULTS AND CONCLUSION: New bone formation was visible in the bone defect regions of the mandible and parietal bone. The amount of lamellar bone formation and the degree of mineralization of the new bone were significantly increased in the parietal bone defect compared with the mandibular bone defect area, indicating the bone remodeling in the parietal bone defect area was better than that in the mandible bone defect area. The integral absorbance values of tartrate-resistant acid phosphatase and bone morphogenetic protein 2 in the parietal bone defect area were lower than those in the mandibular bone defect area, indicating that the viabilities of osteoblasts and osteoclasts in the parietal bone defect area were lower than those in the mandible bone defect area. These results demonstrate that occlusal stimulation may delay the bone remodeling during the repair of mandibular bone defects by regulating bone mineralization and maturation. 
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Parietal Bone, Mandible, Bone Transplantation, Tissue Engineering