中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (45): 7350-7355.doi: 10.3969/j.issn.2095-4344.2015.45.024

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

针对细胞高加速度离心式加载机的研制

葛洪玉1，张春秋1，高丽兰1，张西正2   

  1. 1天津市先进机电系统设计与智能控制重点实验室，天津理工大学机械工程学院，天津市  300384；2军事医学科学院卫生装备研究所，天津市  300000
  • 收稿日期:2015-09-26 出版日期:2015-11-05 发布日期:2015-11-05
  • 通讯作者: 张春秋，博士，教授，天津市先进机电系统设计与智能控制重点实验室，天津理工大学机械工程学院，天津市300384
  • 作者简介:葛洪玉，男，1988年生，山东省人，汉族，天津理工大学在读硕士，主要从事实验室仪器设计分析研究。
  • 基金资助:

    国家自然科学基金资助项目(11432016，11172208，11402171)

Design of a centrifuge device for high acceleration loading on cells

Ge Hong-yu1, Zhang Chun-qiu1, Gao Li-lan1, Zhang Xi-zheng2   

  1. 1Tianjin Key Laboratory of Advanced Electromechanical Systems Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China; 2Institute of Health Equipment, Academy of Military Medical Sciences, Tianjin 300000, China
  • Received:2015-09-26 Online:2015-11-05 Published:2015-11-05
  • Contact: Zhang Chun-qiu, M.D., Professor, Tianjin Key Laboratory of Advanced Electromechanical Systems Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • About author:Ge Hong-yu, Studying for master’s degree, Tianjin Key Laboratory of Advanced Electromechanical Systems Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China
  • Supported by:

    the National Natural Science Foundation of China, No. 11432016, 11172208, 11402171

PDF

793

被引次数

3

摘要：

背景：随着科技和现代航空航天事业的发展，极端力学环境高加速度的力学生物学影响越来越受到重视，研究表明高加速度对细胞有一定的影响。
目的：以离心机为基础，设计离心式加载机用于高加速度加载下细胞的力学生物学研究。
方法：对于离心式加载机，培养板或/和培养瓶加入培养液进行恒加速度或变速度加载，探索实验可行性；另外，根据其转子结构和性能，利用ANSYS有限元分析软件建立其有限元模型，分别在平衡状态下和危险工况下的转子系统进行仿真计算，分析转子系统的应力和变形分布情况。对危险工况下的主轴进行强度校核，并将ANSYS 分析结果与强度校核结果进行对比。
结果与结论：通过实验表明培养板或/和培养瓶可以用于(0-40)×g的恒加速度或变加速度加载；模拟对比分析证明了细胞离心式加载机的可靠性，该加载机实现了一般生物学实验室在高加速度下力学生物学的研究，也为未来细胞离心加载机广泛应用提供了基础。
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 培养, 高加速度, 细胞离心式加载机, 力学生物学, 转子系统, 有限元模型, 强度校核, 可靠性, 国家自然科学基金

Abstract:

BACKGROUND: With the development of science and technology and modern aerospace, the effects of mechanobiology in extreme mechanical environment—high acceleration are becoming an issue of concern. Studies have shown that high acceleration has certain effects on the cells.
OBJECTIVE: Based on a centrifuge, to design a cell loading centrifuge used for exploration of cell mechanobiology under high acceleration.
METHODS: For the cell loading centrifuge, a culture plate or/and culture bottle full of culture fluid was/were loaded with constant acceleration or variable velocity to explore the experimental feasibility. Besides, a finite element model was built by ANSYS software according to structure and properties of the rotor. The rotor system was calculated under equilibrium and dangerous working conditions, respectively, to analyze the stress and deformation distribution. Moreover, the strength of the main shaft was checked under the dangerous working conditions. Then the analysis results of ANSYS were compared with the results of strength check.
RESULTS AND CONCLUSION: Experimental findings showed that the culture plate or/and the culture bottle could be used for (0-40)×g highly constant acceleration or variable acceleration loading. Through the simulation and comparison analysis, we confirmed the reliability of the cell loading centrifuge. This cell loading centrifuge can be used to implement the study of cell mechanobiology under high acceleration in the general biology laboratory. It also provides a basis for wide application of cell loading centrifuge in the future.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Acceleration, Finite Element Analysis, Tissue Engineering