中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (28): 4485-4492.doi: 10.3969/j.issn.2095-4344.2015.28.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

血管内皮生长因子165基因促进人脂肪间充质干细胞的增殖

王 钰1，朱志图2，陈峻江3   

  1. 辽宁医学院附属第一医院，1临床生物样本中心，2肿瘤科，3学科科研处，辽宁省锦州市  121000
  • 出版日期:2015-07-02 发布日期:2015-07-02
  • 通讯作者: 陈峻江，博士，主治医师，辽宁医学院附属第一医院学科科研处，辽宁省锦州市 121000
  • 作者简介:王钰，女，汉族，1979年生，辽宁省辽阳市人， 2013年辽宁医学院毕业，医学硕士。
  • 基金资助:

    辽宁省科技厅科技计划项目(2013225305)

Vascular endothelial growth factor 165 promotes proliferation of human adipose-derived mesenchymal stem cells 

Wang Yu1, Zhu Zhi-tu2, Chen Jun-jiang3   

  1. 1Biobank, 2Oncology Department, 3Subject Research Department, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Chen Jun-jiang, M.D., Attending physician, Subject Research Department, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
  • About author:Wang Yu, Master, Biobank, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
  • Supported by:

    the Science and Technology Plan of Liaoning Science and Technology Department, No. 2013225305 

PDF

419

被引次数

2

摘要：

背景：目前自体脂肪移植已广泛运用于美容整形和修复创伤导致的软组织缺损的修复，有研究表明，移植后1年移植脂肪存活率为20%-80%，因此，在移植后的早期及时、充分的血供建立，对于移植脂肪的存活是非常重要的。

目的：观察血管内皮生长因子165转染人脂肪间充质干细胞的增殖情况。
方法：体外传代培养人脂肪间充质干细胞，将重组血管内皮生长因子165基因入腺病毒液和空病毒液转染至脂肪间充质干细胞内，分别设为实验组和对照组，另设正常培养的细胞为空白组。

结果与结论：RT-PCR，Western blot，MTT检测显示，与对照组和空白组相比，实验组血管内皮生长因子165 mRNA和蛋白的表达及细胞增殖均增高(P < 0.05)。结果证实，腺病毒承载的血管内皮生长因子165基因转染脂肪间充质干细胞后不仅可以持续的表达目的蛋白，同时也能显著促进脂肪间充质干细胞的增殖。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 脂肪干细胞, 血管内皮生长因子165, 脂肪间充质干细胞, 重组腺病毒载体, 脂肪移植, 病毒包装, 血管生成, 成活率, 增强型绿色荧光蛋白, 细胞增殖, 组织缺损

Abstract:

BACKGROUND: Autologous fat transplantation has been widely used in soft tissue defect repair and cosmetic surgery, and the 1-year transplant survival rate is 20%-80%. Therefore, the establishment of timely and adequate blood supply at early period after transplantation is very important for the survival of transplanted fat tissues.

OBJECTIVE: To observe the proliferation of human adipose-derived mesenchymal stem cells transfected with vascular endothelial growth factor 165.
METHODS: Human adipose-derived mesenchymal stem cells were subcultured in vitro. Recombinant adenovirus carrying vascular endothelial growth factor 165 and blank virus were respectively transferred into adipose-derived mesenchymal stem cells. Cells cultured normally served as blank group.
RESULTS AND CONCLUSION: Compared with the control and blank groups, the expressions of vascular endothelial growth factor 165 mRNA and protein were higher in the experimental group (P < 0.05). Experimental findings suggest that the recombinant adenovirus carrying vascular endothelial growth factor 165 transferred into adipose-derived mesenchymal stem cells cannot only maintain the expression of target protein but also obviously promote the proliferation of adipose-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Cell proliferation, Viruses

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