中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (28): 4439-4444.doi: 10.3969/j.issn.2095-4344.2015.28.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

龙鳖胶囊对骨髓间充质干细胞增殖活性的影响

潘建科1，郭柏铭1，刘 军2，张 娴3，谢 辉1，郭 达2   

  1. 1广州中医药大学第二临床医学院，广东省广州市 510405；广东省中医院，2骨科，3中心实验室，广东省广州市 510120
  • 出版日期:2015-07-02 发布日期:2015-07-02
  • 通讯作者: 刘军，主任医师，博士生导师，广东省中医院骨科，广东省广州市 510120
  • 作者简介:潘建科，男，1988年生，广东省兴宁市人，汉族，在读博士，主要从事骨与关节退变及损伤的基础、临床和循证医学研究。
  • 基金资助:

    国家自然科学基金项目(81473698，81273781)；教育部高等学校博士点科研基金项目(20124425110004)；广东省科技计划项目(2011B031700027)；广东省财政厅项目([2014]157号；广东省中医院中医药科学技术研究专项资助(YK2013B2N19)

Effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells

Pan Jian-ke1, Guo Bai-ming1, Liu Jun2, Zhang Xian3, Xie Hui1, Guo Da2   

  1. 1Second School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; 
    2Department of Orthopedics, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China; 
    3Centre Laboratory, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Liu Jun, Chief physician, Doctoral supervisor, Department of Orthopedics, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong Province, China
  • About author:Pan Jian-ke, Studying for doctorate, Second School of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81473698, 81273781; the Specialized Research Fund for the Doctoral Program of Higher Education, Ministry of Education, No. 20124425110004; the Science and Technology Plan of Guangdong Province, No. 2011B031700027; the Project of Guangdong Finance Department, No. [2014]157; the Specialized Fund for Chinese Medicine Research in Guangdong Provincial Hospital of Chinese Medicine, No. YK2013B2N1

PDF

267

被引次数

13

摘要：

背景：临床应用补肾活血中药龙鳖胶囊治疗骨关节炎取得满意的疗效，但其作用机制尚不明确。

目的：观察龙鳖胶囊对大鼠骨髓间充质干细胞增殖活性的影响。
方法：采用全骨髓贴壁培养方法分离培养SD大鼠骨髓间充质干细胞，倒置显微镜进行形态学观察，流式细胞术检测细胞表面标志表达。用含龙鳖胶囊的完全培养基培养第4代骨髓间充质干细胞，药物质量浓度分为5，1 g/L和250，50，10 mg/L，作用24 h后用 MTT 法测定细胞活力。
结果与结论：原代细胞呈现不规则形状贴壁生长，第2代细胞呈典型的纤维状，第3代细胞呈长纤维状、漩涡式生长。第4代骨髓间充质干细胞的表面分子CD29、CD90呈强阳性表达，CD44呈阳性表达，CD45、CD34呈阴性表达。5 g/L 和1 g/L质量浓度的龙鳖胶囊溶液可促进骨髓间充质干细胞增殖。结果表明龙鳖胶囊可能是通过促进骨髓间充质干细胞的增殖和分化，进而发挥治疗骨关节炎的作用。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 骨髓干细胞, 龙鳖胶囊, 骨髓间充质干细胞, 骨关节炎, 补肾活血, MTT, 细胞增殖, 国家自然科学基金

Abstract:

BACKGROUND:Longbie Capsule has satisfactory outcomes in the treatment of osteoarthritis, but its mechanism is still unclear.

OBJECTIVE: To study the effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells.
METHODS: The bone marrow mesenchymal stem cells from SD rats were separated and expanded by whole adherence culture, then subcultured and confirmed by morphological observation and flow cytometry. Passage 4 cells were cultured in complete media containing 5 g/L, 1 g/L, 250 mg/L, 50 mg/L, 10 mg/L Longbie Capsule, respectively, for 24 hours. Then, MTT assay was used to detect cell viability.
RESULTS AND CONCLUSION: The primary cells were adherent cells characterized by irregular shape, passage 2 cells were typically fibrous-shaped, passage 3 cells grew in long fibrous and swirl-type shape. Passage 4 cells were strongly positive for CD29 and CD90, positive for CD44, and negative for CD34 and CD45. 5 g/L and 1 g/L Longbie Capsule promoted the proliferation of bone marrow mesenchymal stem cells. These findings indicate that Longbie Capsule may promote the proliferation and differentiation of bone marrow mesenchymal stem cells, thereby playing a therapeutic effect on osteoarthritis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Osteoarthritis, Bone Marrow, Mesenchymal Stem Cells

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