中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (27): 4379-4383.doi: 10.3969/j.issn.2095-4344.2015.27.021

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

牛磺酸对肝纤维化模型小鼠肝组织超氧化物歧化酶和丙二醛的影响

白  剑1，2，肖  漓1，韩  永1，毕丽丽1，孔祥瑞2，王学良2，张丽萍2，刘喜成3   

  1. 1解放军第三〇九医院器官移植研究所，北京市  100091；
    2辽宁中医药大学，辽宁省沈阳市  110032；
    3深圳市人民医院麻醉科，广东省深圳市  518020
  • 出版日期:2015-06-30 发布日期:2015-06-30
  • 通讯作者: 刘喜成，硕士，深圳市人民医院麻醉科，广东省深圳市 518020
  • 作者简介:白剑，1979年生，辽宁省营口市人，汉族，博士，实验师，主要从事中药对肝脏免疫功能作用的相关研究。
  • 基金资助:

    辽宁省自然科学基金(201101142)，“从脾论治”肝纤维化对相关因子水平影响的实验研究；辽宁省社会发展攻关计划(2012225018)，中医治疗优势病种的关键技术研究及产品开发

The influence of taurine on superoxide dismutase and malondialdehyde in liver tissue of rat models of liver fibrosis

Bai Jian1, 2, Xiao Li1, Han Yong1, Bi Li-li1, Kong Xiang-rui2, Wang Xue-liang2, Zhang Li-ping2, Liu Xi-cheng3   

  1. 1Institute of Organ Transplantation, the 309th Hospital of Chinese People’s Liberation Army, Beijng 100091, China; 
    2Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China; 
    3Department of Anesthesiology, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Liu Xi-cheng, Master, Department of Anesthesiology, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong Province, China
  • About author:Bai Jian, M.D., Institute of Organ Transplantation, the 309th Hospital of Chinese People’s Liberation Army, Beijng 100091, China
  • Supported by:

    the Natural Science Foundation of Liaoning Province of China, No. 201101142; a grant from Key Plan Program of Society Development of Liaoning Province of China, No. 2012225018

PDF

1057

被引次数

10

摘要：

背景：有研究报道牛磺酸对神经系统、心血管系统、免疫系统和消化系统的多种动物疾病模型均有一定的治疗作用。肝脏是牛磺酸代谢的主要场所，也是其重要的靶器官，因此牛磺酸与肝病的关系近年来成为研究热点。
目的：观察牛磺酸对四氯化碳诱导的肝纤维化模型小鼠肝组织中超氧化物歧化酶和丙二醛的影响。
方法：将30只雄性SPF级C57B/L小鼠随机分为空白对照组，模型组和牛磺酸组，每组10只。空白对照组给予100%花生油1 mL/kg腹腔注射，每周2次，共10周；模型组给予含20%四氯化碳的花生油1 mL/kg腹腔注射，每周2次，共10周；牛磺酸组给予含20%四氯化碳的花生油1 mL/kg腹腔注射，每周2次，共10周，并从第3周起给予牛磺酸500 mg/(kg•d)灌胃至第10周。
结果与结论：模型组与空白对照组相比小鼠血清中透明质酸、层粘连蛋白、Ⅲ型前胶原、Ⅳ型胶原、丙氨酸转氨酶、天冬氨酸转氨酶水平显著升高(P < 0.05)，肝脏组织中超氧化物歧化酶水平降低(P < 0.05)，丙二醛水平显著升高(P < 0.05)，肝脏指数增加(P < 0.05)，病理检查显示肝细胞坏死并出现脂肪空泡、纤维组织增生和炎细胞浸润；牛磺酸组与模型组相比小鼠血清中透明质酸、层粘连蛋白、Ⅲ型前胶原、Ⅳ型胶原、丙氨酸转氨酶、天冬氨酸转氨酶水平显著降低(P < 0.05)，肝脏组织中超氧化物歧化酶水平显著升高(P < 0.05)，丙二醛水平显著降低(P < 0.05)，肝脏指数降低(P < 0.05)，病理检查显示肝脏组织中无炎性浸润、脂肪空泡和纤维组织沉积。结果表明牛磺酸可以降低肝纤维化小鼠血清中超氧化物歧化酶和丙二醛含量，通过发挥其抗氧化作用减轻四氯化碳诱导的肝纤维化程度。


中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

关键词: 实验动物, 消化系统损伤动物模型, 牛磺酸, 肝纤维化, 超氧化物歧化酶, 丙二醛, 辽宁省自然科学基金

Abstract:

BACKGROUND: Studies have reported that taurine has a certain therapeutic effect on the disease of various systems, such as nervous system, cardiovascular system, immune system and digestive system. The liver is the main place, also the important target organ, of taurine metabolism. Therefore, the relationship between taurine and hepatopathy has become a hot topic in recent years.
OBJECTIVE: To investigate the influence of taurine on superoxide dismutase and malondialdehyde expression in the liver tissue of rat models of liver fibrosis induced by carbon tetrachloride.
METHODS: Thirty male C57B/L rats of SPF grade were randomly and evenly divided into blank control, model and taurine groups. Rats in the blank control group were intraperitoneally injected with 100% peanut oil of 1 mL/kg, twice a week, in total 10 weeks. Rats in the model group were intraperitoneally injected with peanut oil of 1 mL/kg containing 20% carbon tetrachloride, twice a week, in total 10 weeks. Rats in the taurine group were intraperitoneally injected with peanut oil of 1mL/kg containing 20% carbon tetrachloride, twice a week, in total 10 weeks, and were intragastrically administered taurine of 500 mg/kg per day starting from the 3rd week till the 10th week.
RESULTS AND CONCLUSION: Compared with the blank control group, the serum levels of hyaluronic acid, laminin, type Ⅲ procollagen, type Ⅳ collagen, alanine aminotransferase, aspartate aminotransferase were significantly increased (P < 0.05), the level of superoxide dismutase in the liver tissue was lowered (P < 0.05), the level of malondialdehyde in liver tissue was significantly increased (P < 0.05), and liver index was increased (P < 0.05) in the model group. Pathological examination showed that there were necrosis of liver cells, fat vacuoles, fibrous tissue hyperplasia and inflammatory cell infiltration in the rats of the model group. Compared with the model group, the serum levels of hyaluronic acid, laminin, type Ⅲ procollagen, type Ⅳ collagen, alanine aminotransferase, aspartate aminotransferase were significantly lowered (P < 0.05), the level of superoxide dismutase in the liver tissue was significantly increased (P < 0.05), the level of malondialdehyde in the liver tissue was significantly lowered (P < 0.05), and liver index was significantly decreased (P < 0.05) in the taurine group. Pathological examination showed that there were no inflammatory cell infiltration, fat vacuoles, and fibrous tissue deposition in the liver tissue. The results indicate that taurine can decrease the contents of superoxide dismutase and malondialdehyde, and relieve the degree of liver fibrosis induced by carbon tetrachloride via exerting its antioxidative effects.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Taurine, Cytoprotection, Liver Fibrosis

中图分类号: