中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (12): 1858-1863.doi: 10.3969/j.issn.2095-4344.2015.12.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

加入壳聚糖改善丝素蛋白结晶性：力学稳定强度更好的三维支架材料

张霞只，司徒方民，彭  鹏，焦延鹏   

  1. 暨南大学材料科学与工程系，广东省广州市  510632
  • 修回日期:2015-02-16 出版日期:2015-03-19 发布日期:2015-03-19
  • 通讯作者: 焦延鹏，博士，硕士生导师，研究员，暨南大学材料科学与工程系，广东省广州市 510632
  • 作者简介:张霞只，女，1988年生，河南省安阳市人，汉族，暨南大学在读硕士，主要从事生物材料相关研究。
  • 基金资助:

    国家自然科学基金青年、面上项目(50903039、81101151)；广东省优秀博士论文自助项目(sybzzxm201034)

Chitosan improves the crystallization of silk fibroin: a three-dimensional scaffold material with better mechanical stability

Zhang Xia-zhi, Situ Fang-min, Peng Peng, Jiao Yan-peng   

  1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China
  • Revised:2015-02-16 Online:2015-03-19 Published:2015-03-19
  • Contact: Jiao Yan-peng, M.D., Master’s supervisor, Researcher, Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China
  • About author:Zhang Xia-zhi, Studying for master’s degree, Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 50903039, 81101151; the Research Fund for the Excellent Doctorial Dissertation of Guangdong Province, No. sybzzxm201034

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摘要：

背景：丝素蛋白作为天然生物高分子具有良好的生物相容性，但其结晶性能较高、脆性较大，较难得到均匀结构的三维支架材料。
目的：通过加入壳聚糖改善丝素蛋白的结晶性，得到具有稳定力学强度的三维组织工程支架材料。
方法：采用CaCl2/CH3CH2OH/H2O三元溶液溶解蚕茧，提取丝素蛋白并制成溶液；使壳聚糖溶液与丝素蛋白溶液的质量比分别为2∶1、1∶1、1∶2，采用冷冻干燥法制备多孔丝蛋白/壳聚糖支架材料，并对复合支架进行甲醇浸泡交联处理，以单纯的丝素蛋白和壳聚糖支架为对照。对各组支架进行扫描电镜观察，红外光谱和X射线衍射、孔隙率、吸水率及在水环境下的周期性循环压缩力学性能测试。
结果与结论：复合支架的孔隙结构比纯丝素蛋白支架更均匀有序，并且壳聚糖含量越高，复合支架的孔隙越均匀有序、孔隙率越低、支架结构越致密；当壳聚糖与丝素蛋白的混合比例为1∶2时，在复合支架中的吸水率最高，同时高于丝素蛋白支架，但低于壳聚糖支架；随丝素蛋白成分的增加，复合支架弹性更好，保持形状的能力更优。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 丝素蛋白, 壳聚糖, 复合支架, 性能研究, 国家自然科学基金

Abstract:

BACKGROUND: Silk fibroin as natural biological macromolecules has good biocompatibility, but it is difficult to make the three-dimensional scaffold with uniform structure because of its higher crystallization performance and bigger brittleness.
OBJECTIVE: To improve the crystallization of silk fibroin through the addition of chitosan, and to get three-dimensional tissue engineering scaffolds with better mechanical strength.
METHODS: CaCl2/CH3CH2OH/H2O ternary solution was used to dissolve silkworm cocoon to extract silk fibroin and form solution. Silk fibroin solution and chitosan solution were mixed according to different mixing ratios of 2:1, 1:1, 1:2, respectively, and then porous silk protein/chitosan scaffolds were prepared by freeze-drying method and treated by methanol. Scaffold morphology was observed by scanning electron microscopy, the chemical structure and crystalline state of the scaffolds were characterized through infrared spectrum and X-ray diffraction test, respectively. Also, the porosity and water uptake were tested and periodic cycle compression mechanical properties under the water environment were determined.
RESULTS AND CONCLUSION: The introduction of chitosan could improve the properties of scaffolds. The porosity of the composite scaffold with lower porosity was more uniform and orderly with higher content of chitosan. When the mixture rate of chitosan and silk fibroin was 1:2, the water uptake rate was the highest in the composite scaffolds, and also higher than that of the silk fibroin scaffold but lower than that of the chitosan scaffold. With the increase of silk fibroin, the composite scaffolds had better elasticity and stronger ability to maintain the shape.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: Materials Testing, Chitosan, Silk

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