中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (1): 7-11.doi: 10.3969/j.issn.2095-4344.2015.01.002

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

甲状腺素诱导人脐血间充质干细胞向软骨细胞分化中的作用

杨  涛，江  波，徐  鹏，林  刚   

  1. 南京医科大学附属南京儿童医院，江苏省南京市  210008
  • 修回日期:2014-11-05 出版日期:2015-01-01 发布日期:2015-01-01
  • 通讯作者: 林刚，副主任医师，南京医科大学附属南京儿童医院，江苏省南京市 210008
  • 作者简介:杨涛，男，1989年生，江苏省泰州市人，汉族，南京医科大学在读硕士，主要从事儿童骨科研究。
  • 基金资助:

    南京市卫生局医学科技发展课题(YKK12112)

Thyroxine effects on induced differentiation of human umbilical cord blood-derived mesenchymal stem cells into chondrocytes

Yang Tao, Jiang Bo, Xu Peng, Lin Gang   

  1. Nanjing Children’s Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
  • Revised:2014-11-05 Online:2015-01-01 Published:2015-01-01
  • Contact: Lin Gang, Associate chief physician, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
  • About author:Yang Tao, Studying for master’s degree, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
  • Supported by:

    the Medical Science and Technology Development Project of Nanjing Municipal Health Department, No. YKK12112

PDF

653

被引次数

5

摘要：

背景：研究发现共培养可诱导人源干细胞向特定细胞分化，但如何获得更多的组织工程所需的种子细胞是研究中的难题。
目的：探讨不同浓度甲状腺素在人脐血间充质干细胞向兔软骨细胞分化中的作用。
方法：将人脐血间充质干细胞与兔软骨细胞按照2∶1比例共培养，并用含有不同浓度甲状腺素(0，0.01，0.1，1，10 μmol/L)的培养基分组刺激，共培养14 d后分别观察各组细胞形态，并提取细胞RNA和蛋白质，Real-time PCR检测聚集蛋白多糖mRNA及Ⅱ型胶原mRNA表达量，Western blotting技术检测Ⅱ型胶原及聚集蛋白多糖蛋白表达水平。
结果与结论：加入0.01，0.1，1，10 μmol/L的甲状腺素干预后，聚集蛋白多糖、Ⅱ型胶原mRNA和蛋白的表达水平均随甲状腺素浓度增加而增强，与单纯共培养组比较差异有显著性意义(P < 0.05)。实验证明高浓度甲状腺素可增强人脐血间充质干细胞与兔软骨细胞共培养后向软骨细胞分化的能力。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 脐带脐血干细胞, 共培养, 软骨细胞, 细胞分化, 甲状腺素, Ⅱ型胶原, 聚集蛋白多糖, 软骨组织工程, 种子细胞

Abstract:

BACKGROUND: Human umbilical cord blood-derived mesenchymal stem cells can be induced through the co-culture to differentiate into other cells, but how to get more seed cells for tissue engineering is one of the most difficult problems.
OBJECTIVE: To investigate the role of the different concentrations of thyroxine in chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells by co-culture with rabbit chondrocytes.
METHODS: Human umbilical cord blood-derived mesenchymal stem cells were co-cultured with rabbit chondrocytes at 2:1, and stimulated by medium containing different concentrations of thyroxine (0, 0.01, 0.1 and 1, 10 μmol/L). Co-cultured cells with no thyroxine served as control group. After 14 days of co-culture, the cell RNA and protein were extracted, mRNA expressions of aggrecan and collagen type II were detected by real-time PCR, and protein expression of aggrecan and collagen type II were detected by western blot assay.
RESULTS AND CONCLUSION: After intervention with 0.01, 0.1, 1, 10 μmol/L thyroxine, the mRNA and protein expressions of aggrecan and collagen type II were enhanced with the increase of thyroxine concentration, which were significantly different from those in the control group (P < 0.05). Experimental findings indicate that high levels of thyroxine can enhance the chondrogenic ability of human umbilical cord blood-derived mesenchymal stem cells co-cultured with rabbit chondrocytes.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: Fetal Blood, Mesenchymal Stem Cells, Chondrocytes, Coculture Techniques, Thyroxine

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