中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (45): 7250-7254.doi: 10.3969/j.issn.2095-4344.2014.45.007

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐带间充质干细胞与乳鼠许旺细胞共培养向类神经元样细胞的分化

祝加学1，陶海荣1，沈尊理2   

  1. 1上海交通大学医学院附属第三人民医院骨科，上海市  201999；2上海交通大学附属第一人民医院创伤外科中心，上海市  200080
  • 出版日期:2014-11-05 发布日期:2014-11-05
  • 通讯作者: 陶海荣，上海交通大学医学院附属第三人民医院骨科，上海市 201999
  • 作者简介:祝加学，男，1982年生，山东省兰陵县人，汉族，2010年上海交通大学毕业，硕士，主治医师，主要从事周围神经损伤与修复方面的研究。
  • 基金资助:
    国家自然科学基金(30872630)

Rat’s Schwann cells induce neural differentiation of human umbilical cord mesenchymal stem cells

Zhu Jia-xue1, Tao Hai-rong1, Shen Zun-li2   

  1. 1Department of Orthopedics, Third People’s Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 201999, China; 2Department of Traumatology, First People’s Hospital of Shanghai Jiao Tong University, Shanghai 200080, China
  • Online:2014-11-05 Published:2014-11-05
  • Contact: Tao Hai-rong, Department of Orthopedics, Third People’s Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 201999, China
  • About author:Zhu Jia-xue, Master, Attending physician, Department of Orthopedics, Third People’s Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 201999, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30872630

PDF

745

被引次数

9

摘要：

背景：间充质干细胞可以通过化学诱导或者共培养的方法分化为类神经细胞，但是对于间充质干细胞是与许旺细胞共培养分化为类神经元还是类许旺细胞，还存在争议。

目的：探索大鼠许旺细胞对人脐带间充质干细胞诱导分化作用。

 

方法：将1×109 L-1人脐带间充质干细胞通过Transwell隔离小室与1×109 L-1SD乳鼠许旺细胞共培养2周，观察脐带间充质干细胞的形态变化，并用免疫荧光法检测其表型变化。

 

结果与结论：共培养2周后，部分间充质干细胞出现类似神经元的形态，且表达nestin，NF-200，β-Ⅲ-tubulin等神经元特异性标记物，不表达许旺细胞特异性表记物S100和胶质细胞特异性标记物MAB1580。提示人脐带间充质干细胞可以与大鼠许旺细胞非接触共培养分化为类神经元。

 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 脐带脐血干细胞, 脐带, 间充质干细胞, 许旺细胞, 神经元, 细胞培养, 共培养, 诱导, 分化, 国家自然科学基金

Abstract:

BACKGROUND: Mesenchymal stem cells can differentiate into nerve cells by chemical induction or co-culture method, but whether mesenchymal stem cells co-cultured with Schwann cells differentiate into neuronal-like cells or Schwann-like cells is still controversial.

OBJECTIVE: To explore the inductive role of Schwann cells derived from rats in the differentiation of human umbilical cord mesenchymal stem cells.
METHODS: Cocultures of human umbilical cord mesenchymal stem cells (1×109/L) and Schwann cells (1×109/L) from neonatal rats were performed using transwell culture dishes. After 2 weeks of cocultures, morphology of the cultured human umbilical cord mesenchymal stem cells was observed, and the phenotypic changes of cells were also detected with immumocytochemistry techniques.
RESULTS AND CONCLUSION: After 2 weeks of cocultures, some differentiated cells showed neuron-like morphology, and expressed nestin, NF-200 and β-III-tubulin, but did not express Schwann cell special marker S100 and oligodendrocytes special marker MAB1580. These findings indicate that human umbilical cord mesenchymal stem cells can transdifferentiate into neuronal-like cells by cocultures with rat’s Schwann cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: umbilical cord, mesenchymal stem cells, Schwann cells, cell culture techniques

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