中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (41): 6678-6683.doi: 10.3969/j.issn.2095-4344.2014.41.021

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脐血源神经干细胞移植治疗陈旧性脊髓损伤

吴月奎1，王尚武1，马建华2，易 波2，高兵兵2，秦家振2，杨志军2，戴宜武2   

  1. 1首都医科大学密云教学医院神经外科，北京市 101500；2解放军北京军区总医院附属八一脑科医院，北京市 100700
  • 修回日期:2014-08-25 出版日期:2014-10-01 发布日期:2014-10-01
  • 通讯作者: 戴宜武，教授，主任医师，博士生导师，解放军北京军区总医院附属八一脑科医院，北京市 100700
  • 作者简介:吴月奎，男，1986年生，安徽省枞阳县人，汉族，硕士，主治医师，主要从事神经创伤的基础及临床研究。
  • 基金资助:

    国家自然科学基金(81271391)

Umbilical cord blood neural stem cells for obsolete spinal cord injury    

Wu Yue-kui1, Wang Shang-wu1, Ma Jian-hua2, Yi Bo2, Gao Bing-bing2, Qin Jia-zhen2, Yang Zhi-jun2, Dai Yi-wu2   

  1. 1Department of Neurosurgery, Miyun Hospital Affiliated to Capital Medical University, Beijing 101500, China; 2Bayi Brain Hospital Affiliated to the General Hospital of Beijing Military Region, Beijing 100700, China
  • Revised:2014-08-25 Online:2014-10-01 Published:2014-10-01
  • Contact: Dai Yi-wu, Professor, Chief physician, Doctoral supervisor, Bayi Brain Hospital Affiliated to the General Hospital of Beijing Military Region, Beijing 100700, China
  • About author:Wu Yue-kui, Master, Attending physician, Department of Neurosurgery, Miyun Hospital Affiliated to Capital Medical University, Beijing 101500, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81271391

PDF

534

被引次数

2

摘要：

背景：随着医学的进步，脊髓损伤患者的预后未得到明显的改善，大部分患者常遗留严重的并发症。如今，许多实验室和临床研究成果已经提示细胞治疗具有巨大潜能，特别是脐血干细胞在神经系统疾病中的应用。

目的：观察脐血源神经干细胞治疗陈旧性脊髓损伤的可行性和临床疗效。
方法：无菌条件下采集新生儿脐血，在体外条件下分化成神经干细胞，制成细胞浓度为109 L-1的细胞悬液，经腰椎穿刺(L3-L4或L4-L5)注入蛛网膜下腔3 mL。移植前和移植后3个月对脊髓损伤患者行脊髓损伤神经学分类国际标准(ASIA)评分和膀胱残余尿量测定。
结果与结论：脐血源神经干细胞移植后患者生命体征平稳，移植后3个月ASIA各项评分较前提高、膀胱残余尿量减少，差异有显著性意义(P < 0.05)。可见脐血源神经干细胞移植作为一种新的治疗方法，可以改善陈旧性脊髓损伤患者肢体功能，提高患者生活质量。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 移植, 脐血源神经干细胞, 脊髓损伤, 膀胱残余尿量, ASIA评分, 国家自然科学基金

Abstract:

BACKGROUND: With the medical development, prognostic outcomes of spinal cord injuries have not been improved significantly, and most patients also suffer from severe complications. Nowadays, lots of laboratories and clinical researches have suggested that cell therapy has a great potential, especially the application of umbilical cord blood stem cells in nervous system diseases.

OBJECTIVE: To explore the feasibility and clinical effect of umbilical cord blood neural stem cells transplantation for patients with obsolete spinal cord injury.
METHODS: Umbilical cord blood was harvested from newborns under aseptic condition, and differentiated into neural stem cells in vitro that were prepared into cell suspension at a concentration of 109/L. The cell suspension (3 mL) was injected via the L3-4 or L4-5 into the subarachnoid space. American Spinal Injury Association (ASIA) scores and the residual urine were assessed before and 3 months after transplantation.
RESULTS AND CONCLUSION: After transplantation, all the patients showed a stable life indication. Three months later, ASIA scores were increased and the residual urine decreased, which significantly differed from those before transplantation (P < 0.05). These findings indicate that umbilical cord blood neural stem cells transplantation is a new treatment that can improve the limb function and life quality of patients with obsolete spinal cord injury.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: spinal cord injuries, neural stem cells, fetal blood, treatment outcome

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