中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (37): 6029-6033.doi: 10.3969/j.issn.2095-4344.2014.37.023

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

鸡卵清提取液中大于3 ku蛋白促细胞升高表达多能基因Oct-3/4和Nanog

阮光萍，姚  翔，刘菊芬，舒  帆，王金祥，何  洁，杨建勇，赵  晶，庞荣清，潘兴华   

  1. 解放军昆明总医院干细胞工程实验室，云南省昆明市  650032
  • 修回日期:2014-08-02 出版日期:2014-09-03 发布日期:2014-09-03
  • 通讯作者: 潘兴华，博士，主任医师，解放军昆明总医院干细胞工程实验室，云南省昆明市 650032
  • 作者简介:阮光萍，女，1974年生，云南省昆明市人，汉族，2007年解放军第一军医大学毕业，博士，副主任医师，主要从事干细胞相关研究。
  • 基金资助:

    国家自然科学基金(31172170)；国家重点基础研究发展计划(973计划)(2012CB518106)；云南省高新技术产业发展专项(201204)资助的课题

More than 3 ku proteins in chicken egg extract up-regulate expression of pluripotent genes Oct-3/4 and Nanog

Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Shu Fan, Wang Jin-xiang, He Jie, Yang Jian-yong, Zhao Jing, Pang Rong-qing, Pan Xing-hua   

  1. Stem Cell Engineering Laboratory, Kunming General Hospital of PLA, Kunming 650032, Yunnan Province, China
  • Revised:2014-08-02 Online:2014-09-03 Published:2014-09-03
  • Contact: Pan Xing-hua, M.D., Chief physician, Stem Cell Engineering Laboratory, Kunming General Hospital of PLA, Kunming 650032, Yunnan Province, China
  • About author:Ruan Guang-ping, M.D., Associate chief physician, Stem Cell Engineering Laboratory, Kunming General Hospital of PLA, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31172170; the National Key Basic Research and Development Program of China (973 Program), No. 2012CB518106; Special Project of High-New Technology Industrial Development in Yunnan Province, No. 201204

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545

被引次数

1

摘要：

背景：将体细胞重编程为多能干细胞在生物医学研究领域有广泛的应用价值。
目的：分析鸡卵清提取液中不同分子质量的蛋白促进293T细胞升高表达多能基因Oct-3/4和Nanog的作用。
方法：分离鸡卵清提取液中大于3 ku和小于3 ku的成分，用于293T细胞共培养。实验分为4组：每孔加入1×105个293T细胞，总体积500 μL。对照组加入500 μL培养基；另外3个孔分别加500 μL鸡卵清提取液、鸡卵清大于3 ku的成分和小于3 ku的成分。采用定量PCR检测多能基因Nanog和Oct-3/4的相对表达量。
结果与结论：用共培养法大于3 ku的成分有促细胞升高表达多能基因Oct-3/4和Nanog的作用，但小于3 ku的成分没有促细胞升高表达多能基因的作用。提示在鸡卵清提取液中促细胞升高表达多能基因的成分是大于  3 ku的蛋白质。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 培养, 鸡卵清提取液, 多能基因, 大于3 ku的成分, 293T细胞, 细胞培养, 国家自然科学基金

Abstract:

BACKGROUND: Reprogramming somatic cells to generate pluripotent stem cells has a wide application in biomedical research.
OBJECTIVE: To analyze the effect of different molecular weight proteins in chicken egg-white extract to elevate expression of pluripotent genes Oct-3/4 and Nanog in 293T cells.
METHODS: The extracts of chicken egg-white were separated into more than 3 ku and less than 3 ku ingredients to be used for co-culture with 293T cells. There were four groups, 1×105 293T cells per well, total 500 μL. In the control group, 500 μL culture medium was added; in the other three groups, 500 μL chicken egg-white extract, more than 3 ku and less than 3 ku ingredients were respectively added. Quantitative PCR was used to determine the relative expression levels of pluripotent genes Nanog and Oct-3/4 in 293T cells.
RESULTS AND CONCLUSION: By using co-culture method, more than 3 ku ingredients have a role to increase the expression of pluripotent genes Oct-3/4 and Nanog, but less than 3 ku ingredients cannot elevate the expression of pluripotent genes. This indicates that the ingredient of chicken egg-white extract to elevate the expression of pluripotent genes is more than 3 ku proteins.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: chickens, genes, hek293 cells

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