中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (37): 5916-5922.doi: 10.3969/j.issn.2095-4344.2014.37.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

经冠状静脉逆行灌注碱性成纤维细胞生长因子对骨髓间充质干细胞体内分化的影响

王  晓1，甄  雷1，缪黄泰1，吴星欣1，任红梅1，师树田1，乔  岩2，刘新民2，阙  斌1，聂绍平1   

  1. 首都医科大学附属北京安贞医院，1急诊危重症中心，2心内科，北京市  100029
  • 修回日期:2014-08-07 出版日期:2014-09-03 发布日期:2014-09-03
  • 通讯作者: 聂绍平，博士，教授，主任医师，首都医科大学附属北京安贞医院急诊危重症中心，北京市 100029
  • 作者简介:王晓，男，1985年生，山东省淄博市人，汉族，2011年首都医科大学毕业，硕士，医师，主要从事冠心病的基础与临床研究。
  • 基金资助:

    国家自然科学基金面上项目(81070166，81270284)

Effects of basic fibroblast growth factor via coronary venous retroperfusion on bone marrow mesenchymal stem cell differentiation in vivo

Wang Xiao1, Zhen Lei1, Miao Huang-tai1, Wu Xing-xin1, Ren Hong-mei1, Shi Shu-tian1, Qiao Yan2,Liu Xin-min2, Que Bin1, Nie Shao-ping1   

  1. 1Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; 2Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Revised:2014-08-07 Online:2014-09-03 Published:2014-09-03
  • Contact: Nie Shao-ping, M.D., Professor, Chief physician, Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • About author:Wang Xiao, Master, Physician, Emergency & Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81070166, 81270284

PDF

422

被引次数

2

摘要：

背景：体外研究显示，碱性成纤维细胞生长因子能促进骨髓间充质干细胞向心肌样细胞分化。然而，在体内环境下经冠状静脉逆行灌注碱性成纤维细胞生长因子能否促进骨髓间充质干细胞分化尚不明确。
目的：探讨经冠状静脉逆行灌注碱性成纤维细胞生长因子对骨髓间充质干细胞在体内分化的影响。
方法：①杂种犬12只，采用密度梯度离心与贴壁培养法在体外分离、培养骨髓间充质干细胞，增强型绿色荧光蛋白慢病毒载体转染骨髓间充质干细胞并分析转染率。②开胸结扎法建立急性心肌梗死模型，1周后将存活犬(n=10)随机分为骨髓间充质干细胞组(n=5)和碱性成纤维细胞生长因子+骨髓间充质干细胞组(n=5)，采用OTW球囊经冠状静脉逆行灌注碱性成纤维细胞生长因子和增强型绿色荧光蛋白标记的骨髓间充质干细胞。移植后1周，免疫荧光法比较两组梗死心肌内增强型绿色荧光蛋白阳性细胞共表达Ⅷ因子和肌钙蛋白I的数量。
结果与结论：增强型绿色荧光蛋白慢病毒成功转染骨髓间充质干细胞，转染率达85%；灌注后免疫荧光显示，23.5%的切片可以看到增强型绿色荧光蛋白标记的骨髓间充质干细胞阳性细胞；碱性成纤维细胞生长因子+骨髓间充质干细胞组增强型绿色荧光蛋白共表达Ⅷ因子和肌钙蛋白I的细胞数量明显高于骨髓间充质干细胞组(P < 0.05)。因此，经冠状静脉逆行灌注碱性成纤维细胞生长因子能更有效地促进骨髓间充质干细胞分化成血管内皮细胞和心肌细胞；采用该途径联合灌注碱性成纤维细胞生长因子和骨髓间充质干细胞有望发挥协同作用，更好地促进心脏修复。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 骨髓干细胞, 冠状静脉逆行灌注, 骨髓间充质干细胞, 细胞移植, 碱性成纤维细胞生长因子, 分化, 急性心肌梗死, 国家自然科学基金

Abstract:

BACKGROUND: In vitro studies have demonstrated that basic fibroblast growth factor (bFGF) promote the differentiation of bone marrow mesenchymal stem cells (BMSCs) into cardiomyocyte-like cells. However, it is unclear whether coronary venous retroperfusion of bFGF stimulates BMSCs differentiation in vivo.
OBJECTIVE: To evaluate the effects of coronary venous retroperfusion of bFGF on BMSCs differentiation in vivo.
METHODS: BMSCs from 12 dogs were isolated by density gradient centrifugation and expanded in vitro. These cells were transfected by enhanced green fluorescence protein (EGFP) lentiviral vector and the transfection efficiency was analyzed. Acute myocardial infarction was induced by ligation of left anterior descending coronary artery. After 1 week, 10 survival animals were randomized to BMSCs group (n=5) and bFGF+BMSCs group (n=5).bFGF- and EGFP-positive BMSCs were reversely infused via coronary vein using over-the-wire balloon catheter. One week after infusion, the number of EGFP-positive cells co-staining factor VIII and troponin I was compared between the two groups by immunofluorescence method.
RESULTS AND CONCLUSION: BMSCs were successfully transfected by EGFP and the transfection efficiency was 85%. Immunofluorescence showed that EGFP-positive BMSCs were observed in 23.5% of slides. There were more EGFP-positive cells co-staining VIII and troponin I in the bFGF+BMSCs group than in the BMSCs group (P < 0.05). Thus, the coronary venous retroperfusion of bFGF enhances the differentiation of BMSCs into vascular endothelial cells and cardiomyocytes. Combined delivery of bFGF and BMSCs can exert a synergistic effect to promote cardiac repair.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, fibroblast growth factor 2, myocardial infarction

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