中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (36): 5781-5787.doi: 10.3969/j.issn.2095-4344.2014.36.008

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

构建创伤性股骨头塌陷坏死大鼠模型及阿仑膦酸钠预防的机制

辛大森1，2，范  猛2，姜文学2   

  1. 1天津医科大学一中心临床学院骨科，天津市  300192
    2天津市第一中心医院骨科，天津市  300192
  • 修回日期:2014-07-11 出版日期:2014-08-30 发布日期:2014-08-30
  • 通讯作者: 姜文学，博士，主任医师，天津市第一中心医院骨科，天津市 300192
  • 作者简介:辛大森，男，1987年生，河北省沧州市人，汉族，天津医科大学在读硕士，主要从事股骨头坏死及人工关节的研究。
  • 基金资助:

    天津市卫生局科技基金(2012KY09，锶盐预防股骨头坏死塌陷的实验研究)；天津市卫生局科技基金重点攻关课题(KG109，骨软骨再生技术治疗股骨头坏死的研究)

Constructing a rat model of traumatic osteonecrosis of the femoral head with articular surface collapse and prevention mechanism of alendronate

Xin Da-sen1, 2, Fan Meng2, Jiang Wen-xue2   

  1. 1Department of Orthopedics, First Central Clinical College, Tianjin Medical University, Tianjin 300192, China
    2Department of Orthopedics, Tianjin First Central Hospital, Tianjin 300192, China
  • Revised:2014-07-11 Online:2014-08-30 Published:2014-08-30
  • Contact: Jiang Wen-xue, M.D., Chief physician, Department of Orthopedics, Tianjin First Central Hospital, Tianjin 300192, China
  • About author:Xin Da-sen, Studying for master’s degree, Department of Orthopedics, First Central Clinical College, Tianjin Medical University, Tianjin 300192, China; Department of Orthopedics, Tianjin First Central Hospital, Tianjin 300192, China
  • Supported by:

    the Science and Technology Project of Tianjin Public Health Bureau, No. 2012KY09; the Key Science and Technology Topic of Tianjin Public Health Bureau, No. KG109

PDF

600

被引次数

4

摘要：

背景：临床随访研究表明阿仑膦酸钠对于预防股骨头坏死塌陷有效，但尚缺乏其预防塌陷作用的机制研究。
目的：分析阿仑膦酸钠预防股骨头坏死塌陷的效果及其作用机制。
方法：将45只SD大鼠随机分成3组，每组15只。安慰剂组在建立股骨头坏死模型后给予生理盐水治疗；阿仑膦酸钠组建立股骨头坏死模型后给予药物阿仑膦酸钠治疗；假手术组给予同样剂量的生理盐水治疗。造模后5周处死大鼠，取造模侧股骨标本分别行大体标本观察，X射线、Micro-CT及组织学检测。
结果与结论：大体标本观察安慰剂组股骨头明显塌陷畸形，阿仑膦酸钠组股骨头轻度变形。股骨头高度与宽度的比值假手术组>阿仑膦酸钠组>安慰剂组，差异均有显著性意义。Micro-CT扫描结果显示阿仑膦酸钠组骨小梁平均数量多于安慰剂组，少于假手术组，差异均有显著性意义。阿仑膦酸钠组骨小梁平均厚度小于安慰剂组，但和假手术组比差异无显著性意义。阿仑膦酸钠组骨小梁平均间距小于安慰剂组，但大于假手术组，差异均有显著性意义。阿仑膦酸钠组股骨头骨组织体积、骨表面积、骨矿盐密度均大于安慰剂组，小于假手术组，差异均有显著性意义。组织学检测结果显示，阿仑膦酸钠组存在明显的死骨，破骨细胞明显受到抑制，破骨细胞数量较安慰剂组明显减少，成骨细胞和新生血管也受到了一定程度的抑制。结果表明阿仑膦酸钠可通过全面抑制破骨细胞、成骨细胞及血管新生而抑制骨坏死的修复反应，减慢坏死骨的吸收，保存骨量及股骨头形态，对大鼠创伤性股骨头坏死早期塌陷具有一定的预防作用。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 实验动物, 组织构建, 股骨头坏死, 塌陷, 阿仑膦酸钠, 成骨细胞, 破骨细胞

Abstract:

BACKGROUND: Clinical follow-up studies have shown that alendronate is effective to prevent femoral head collapse following traumatic osteonecrosis of the femoral head, but its mechanism remains poorly understood.
OBJECTIVE: To analyze the effects and mechanisms of alendronate on prevention of collapse in traumatic osteonecrosis of the femoral head.
METHODS: Totally 45 Sprague-Dawley rats were randomly divided into three groups, with 15 rats in each group. Placebo group received physiological saline after establishing models of avascular necrosis of the femoral head. Alendronate group received treatment of alendronate after model induction. Sham surgery group received an equal volume of physiological saline. At 5 weeks after model establishment, the rats were sacrificed. Femoral samples at the modeling side were collected for general observation, X-ray irradiation, Micro-CT and histological detection.
RESULTS AND CONCLUSION: General observation revealed that the femoral head was obviously deformed in the placebo group, but mild deformity was visible in the alendronate group. The ratio of height to width of the femoral head ranked as follows: sham surgery group > alendronate group > placebo group, showing significant differences. Micro-CT scanning results demonstrated that the mean number of bone trabecula was more in the alendronate group than in the placebo group, but less than sham surgery group, showing significant differences. The mean thickness of bone trabecula was less in the alendronate group than in the placebo group, but no significant difference was detectable as compared with the sham surgery group. The mean spacing of bone trabecula was less in the alendronate group than in the placebo group, but larger than in the sham surgery group, showing significant differences. Bone volume, bone surface area and bone mineral density were larger in the alendronate group than in the placebo group, but less than sham surgery group, showing significant differences. Histological detection results demonstrated that apparent sequestrum existed and osteoclasts were obviously inhibited in the alendronate group. The number of osteoclasts was noticeably less in the alendronate group than in the placebo group. Osteoblasts and new vessels were suppressed to some degrees. Results suggested that alendronate can inhibit curing reaction through inhibiting osteoclast and osteoblast activity and vessel formation, which can finally slow down the absorption of necrotic bone and preserve femoral head mass and shape. Thus, alendronate can be used as a preventive against femoral head collapse in rats with traumatic osteonecrosis of the femoral head.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: femur head necrosis, osteoblasts, osteoclasts

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