中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (19): 3030-3035.doi: 10.3969/j.issn.2095-4344.2014.19.013

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

不同代次大鼠脂肪间充质干细胞增殖及成神经球的能力比较

张恩丰，曹  锐，徐  韬，王晓燕，买尔旦•买买提，王国旗，盛伟斌   

  1. 新疆医科大学第一附属医院脊柱外科，新疆维吾尔自治区乌鲁木齐市  830054
  • 修回日期:2013-12-15 出版日期:2014-05-07 发布日期:2014-05-07
  • 通讯作者: 盛伟斌，主任医师，新疆医科大学第一附属医院脊柱外科，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:张恩丰，男，1986年生，新疆维吾尔自治区阜康市人，汉族，新疆医科大学在读硕士，主要从事脊髓损伤的基础与临床研究。
  • 基金资助:

    国家自然科学基金(81060106)；新疆研究生科研创新项目(XJGRI2013087)

Comparative study of proliferative and neurosphere differentiation capacities of adipose-derived mesenchymal stem cells at different passages

Zhang En-feng, Cao Rui, Xu Tao, Wang Xiao-yan, Maierdan Maimaiti, Wang Guo-qi, Sheng Wei-bin   

  1. Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Revised:2013-12-15 Online:2014-05-07 Published:2014-05-07
  • Contact: Sheng Wei-bin, Chief physician, Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Zhang En-feng, Studying for master’s degree, Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81060106; Xinjiang Graduate Research Innovation Project, No. XJGRI2013087

PDF

545

被引次数

2

摘要：

背景：脂肪间充质干细胞是一组具有多向分化潜能的干细胞，体外在一定条件下可分化为神经干细胞。
目的：观察不同代次大鼠脂肪间充质干细胞体外培养增殖能力及诱导成神经球的潜力。
方法：取SD大鼠脂肪体外分离培养出脂肪间充质干细胞并传代扩增，分别在P3、P6、P10、P20时观察其形态学变化、测定增殖速度。流式细胞仪检测定不同代次细胞表型及细胞周期。将脂肪间充质干细胞诱导为神经球，计数成神经球率。
结果与结论：脂肪间充质干细胞主要呈长梭形，不同代次的脂肪间充质干细胞均具有很强的体外增殖能力；除P3细胞其他代次细胞均高表达CD29、CD44、CD73，低表达CD45、CD34。细胞周期中G0/G1期细胞所占比例P3为93.4%、P6为92.7%、P10为92.4%、P20为86.0%。P6、P10诱导成神经球率均显著高于P20(P < 0.05)，P3细胞较难诱导成神经球。提示以脂肪间充质干细胞作为种子细胞时，应注意选择适宜的扩增代数，以便在获得足够纯度细胞的同时保留细胞最佳的分化潜力。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

关键词: 干细胞, 脂肪干细胞, 脂肪间充质干细胞, 不同代次, 细胞培养, 细胞分化, 神经球, 国家自然科学基金 

Abstract:

BACKGROUND: Adipose-derived mesenchymal stem cells are a group of pluripotent stem cells, and under certain conditions can differentiate into neural stem cells in vitro.
OBJECTIVE: To investigate the proliferative and differentiation ability of different passage mesenchymal stem cell from adipose tissue into neurospheres.
METHODS: The adipose-derived mesenchymal stem cells from Sprague-Dawley rats were separated and cultured in vitro, and morphology and proliferation rate of cells were observed and compared respectively at passages 3, 6, 10 and 20. The cell surface antigens and cells cycle were identified by flow cytometry. Furthermore, adipose-derived mesenchymal stem cells were induced into neurospheres, and the neurosphere rate was counted.
RESULTS AND CONCLUSION: Adipose-derived mesenchymal stem cells were mainly in long spindle shape, and cells at different passages had highly proliferative capacity in vitro. Except passage 3, adipose-derived mesenchymal stem cells strongly expressed CD29, CD44, CD73 and lowly expressed CD45 and CD34. The proportion of G0/G1 phase in cell cycle was 93.4% at passage 3, 92.7% at passage 6, 92.4% at passage 10, 86.0% at passage 20. Adipose-derived mesenchymal stem cells at passages 6 and 10 were easier to differentiate into neurospheres than those at passage 20 (P < 0.05), but cells at passage 3 were difficult to differentiate into neurospheres. Therefore, when using adipose-derived mesenchymal stem cells as seed cells, we should pay attention to choose the appropriate amplification passage in order to obtain the cells with best differentiation potential and cell purity.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: stem cells, mesenchymal stem cells, neural stem cells, neurons

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