中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (19): 3443-3448.doi: 10.3969/j.issn.2095-4344.2013.19.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞干预再生障碍性贫血患者树突状细胞的成熟分化

张乐琴,肖 扬,蒋祖军,肖浩文,李 力,庞 妍   

  1. 解放军广州军区广州总医院,广东省广州市  510010
  • 收稿日期:2012-06-09 修回日期:2012-08-28 出版日期:2013-05-07 发布日期:2013-05-07
  • 通讯作者: 肖扬,主任医师,硕士生导师,解放军广州军区广州总医院,广东省广州市 510010
  • 作者简介:张乐琴★,女,1980年生,广西壮族自治区南宁市人,汉族,2010年广州医学院毕业,硕士,医师,主要从事血液病临床研究。 lele9910@sina.com

Bone marrow mesenchymal stem cells intervene maturation and differentiation of dendritic cells in aplastic anemia patients

Zhang Le-qin, Xiao Yang, Jiang Zu-jun, Xiao Hao-wen, Li Li, Pang Yan   

  1. General Hospital of Guangzhou Military Command of PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2012-06-09 Revised:2012-08-28 Online:2013-05-07 Published:2013-05-07
  • Contact: Xiao Yang, Chief physician, Master’s supervisor, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China
  • About author:Zhang Le-qin★, Master, Physician, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China lele9910@sina.com

摘要:

背景:骨髓间充质干细胞对再生障碍性贫血患者T细胞增殖的影响国内仅见少量报道,而骨髓间充质干细胞是否通过调节树突状细胞来影响再生障碍性贫血患者T细胞的增殖,国内未见报道,其机制值得深入研究。
目的:观察骨髓间充质干细胞对再生障碍性贫血患者树突状细胞的免疫调节作用。
方法:将培养第5天的再生障碍性贫血患者外周血单个核细胞来源的树突状细胞与第3代健康人骨髓间充质干细胞混合培养,加入脂多糖、肿瘤坏死因子促树突状细胞成熟,应用流式细胞仪检测骨髓间充质干细胞与未成熟、成熟树突状细胞共培养前后树突状细胞表面标志表达。
结果与结论:未成熟的树突状细胞在脂多糖的刺激诱导下与骨髓间充质干细胞共培养前后,树突状细胞表面标志CD14,CD1a,CD83,CD80表达无变化(P > 0.05);成熟树突状细胞与骨髓间充质干细胞共培养前后,树突状细胞表面标志CD14,CD1a,CD83,CD80表达降低(P < 0.05)。结果说明骨髓间充质干细胞可抑制再生障碍性贫血患者单核细胞来源的树突状细胞的发育和成熟,进而发挥调节再生障碍性贫血患者的免疫作用。

关键词: 干细胞, 骨髓干细胞, 贫血, 再生障碍性, 骨髓, 间充质干细胞, 共培养, 单个核细胞, 树突状细胞, 免疫表型, 抑制, 免疫调节

Abstract:

BACKGROUND: There are few reports about the influence of bone marrow mesenchymal stem cells on T cell proliferation in aplastic anemia patients in China. Besides, no study is reported in China on the topic that whether bone marrow mesenchymal stem cells affect the T cell proliferation in aplastic anemia patients by dendritic cell regulation. Therefore, its mechanism deserves further investigations.
OBJECTIVE: To explore effects of human bone marrow mesenchymal stem cells on dendritic cell immune regulation in patients with aplastic anemia.
METHODS: Human peripheral blood mononuclear cells-derived dendritic cells cultured for 5 days were co-cultured with human bone marrow mesenchymal stem cells at passage 3. Lipopolysaccharide and tumor necrosis factor were added to promote the maturation of dendritic cells. Flow cytometry was used to detect the expression of dendritic cell surface markers before and after coculture of bone marrow mesenchymal stem cells and immature and mature dendritic cells.
RESULTS AND CONCLUSION: Under the induction of lipopolysaccharide, prior to and following the coculture of immature dendritic cells and bone marrow mesenchymal stem cells, no changes of the expression of CD14, CD1a, CD83 and CD80 were observed (P > 0.05). Prior to and following the coculture of mature dendritic cells and bone marrow mesenchymal stem cells, the expression of CD14, CD1a, CD83 and CD80 was decreased (P < 0.05). Results suggested that bone marrow mesenchymal stem cells can inhibit the development and maturation of dendritic cells derived from monocytes of aplastic anemia patients, and exhibit immunoregulatory effects in patients with aplastic anemia.

Key words: stem cells, bone marrow-derived stem cells, anemia, aplastic, bone marrow, mesenchymal stem cells, coculture, mononuclear cells, dendritic cells, immunophenotype, inhibition, immunoregulation

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